PHYTOCHEMICAL, ANTIOXIDANT, AND TRACE ELEMENT ANALYSIS OF ANTHELMINTIC PLANTS OF NORTH-EAST INDIA
Objective: Cynodon dactylon, Hibiscus rosa-sinensis, Elaeocarpus floribundus and Acorus calamus are anthelmintic medicinal plants of north-east India. Due to its pharmacological properties, the present study was aimed to investigate the phytochemical, antioxidant and heavy metal content of the plants.
Methods: Preliminary phytochemical screening was carried out for the presence of phenolics, flavonoids, proteins, and carbohydrates following standard protocols. The antioxidant potential of plants was studied by phosphomolybdate, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. The heavy metal content was estimated using atomic absorption spectrophotometer (Perkin Elmer 3110).
Results: The result showed that the alcoholic extracts of the plant sample contain high quantity of proteins, carbohydrates, phenolics, and flavonoids. Antioxidant assays revealed appreciable free radical scavenging potential. The total antioxidant activity was highest in E. floribundus (62.48Â±1.53 Âµg/mg extract) while C. dactylon showed lowest (34.00Â±0.32 Âµg/mg extract). Similarly, DPPH and FRAP study also showed high in E. floribundus and lowest in H. rosa-sinensis and A. calamus. Elemental analysis of the plant extracts showed a high content of Mn in all the plants while Co was found to be lowest. The tested plants showed low levels of toxic elements.
Conclusion: The high quantity of nutritional content and antioxidant property suggest that plants may be a good source of phytomedicine. Less quantity of toxic elements offers an opportunity to explore the medicinal application of the plants. However, further study needs to be carried out to know its mode of action.
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