PRE-CLINICAL STUDIES ON DIABETES MELLITUS USING PARTIAL PANCREATECTOMY IN SWISS ALBINO MICE

  • Shibu Narayan Jana Department of Pharmaceutical Sciences and Technology, BIT, Mesra, Ranchi http://orcid.org/0000-0001-8929-3492
  • Papiya Mitra Mazumder Department of Pharmaceutical Sciences and Technology, BIT, Mesra, Ranchi

Abstract

Objective: The present study was aimed at the development of partial pancreatectomy in a murine model for diabetes.

Methods: Diabetes model was successfully developed by partial pancreatectomy method. In this study, cyclosporine was used for influenced the glycaemic status. Diabetes status was evaluated by fasting blood glucose level (FBG), lipid profile (by estimation of total cholesterol level and HDL-level), serum amylase and biochemical assay like glutathione estimation.

Results: We report here the restoration of euglycaemic status in cyclosporine-induced diabetic in swiss albino mice after 30% pancreatectomy. In this study, Pancreatectomised (partial) group of animals showed a rapid elevation of glycaemic status, starting from 15th post observational d, but the level of significance decreased gradually from 15th (P<0.01) to 60th (P<0.05) d. This was probably due to nesidioblastotic activity which shifted the fasting blood glucose level towards normoglycaemic status with β-cells regeneration. Cyclosporine treated a group of mice showed normoglycaemic status throughout the whole experimental period, but the cholesterol level remained significant (P<0.001) till the end of the experimental d. Gradually decrements in glycaemia of the diabetic pancreatectomised animals demonstrate islets neogenesis occurring after the operative activity, leading to normoglycaemic condition, probably attributed to β-cells proliferation.

Conclusion: The biochemical and histopathological evaluations suggest that there is the development of the diabetic model in the pancreatectomized group and diabetes status induced by pancreatectomy is curable to a certain extent due to the regeneration of β-cells.

Keywords: Diabetes, Partial pancreatectomy, Euglycaemic status, Nesidioblastotic activity, Glycaemia, Normoglycaemic status, β-cells proliferation

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Author Biographies

Shibu Narayan Jana, Department of Pharmaceutical Sciences and Technology, BIT, Mesra, Ranchi
SHIBU NARAYAN JANA, BIRLA INSTITUTE OF TECHNOLOGY, MESRA, RANCHI, DEPARTMENT PHARMACEUTICAL SCIENCES AND TECHNOLOGY 835215.
Papiya Mitra Mazumder, Department of Pharmaceutical Sciences and Technology, BIT, Mesra, Ranchi
PAPIYA MITRA MAZUMDER, BIRLA INSTITUTE OF TECHNOLOGY, MESRA, RANCHI, DEPARTMENT PHARMACEUTICAL SCIENCES AND TECHNOLOGY 835215.

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How to Cite
Jana, S. N., and P. M. Mazumder. “PRE-CLINICAL STUDIES ON DIABETES MELLITUS USING PARTIAL PANCREATECTOMY IN SWISS ALBINO MICE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 1, Jan. 2018, pp. 71-76, doi:10.22159/ijpps.2018v10i1.21250.
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