PRE-CLINICAL STUDIES ON DIABETES MELLITUS USING PARTIAL PANCREATECTOMY IN SWISS ALBINO MICE


Shibu Narayan Jana, Papiya Mitra Mazumder

Abstract


Objective: The present study was aimed at the development of partial pancreatectomy in a murine model for diabetes.

Methods: Diabetes model was successfully developed by partial pancreatectomy method. In this study, cyclosporine was used for influenced the glycaemic status. Diabetes status was evaluated by fasting blood glucose level (FBG), lipid profile (by estimation of total cholesterol level and HDL-level), serum amylase and biochemical assay like glutathione estimation.

Results: We report here the restoration of euglycaemic status in cyclosporine-induced diabetic in swiss albino mice after 30% pancreatectomy. In this study, Pancreatectomised (partial) group of animals showed a rapid elevation of glycaemic status, starting from 15th post observational d, but the level of significance decreased gradually from 15th (P<0.01) to 60th (P<0.05) d. This was probably due to nesidioblastotic activity which shifted the fasting blood glucose level towards normoglycaemic status with β-cells regeneration. Cyclosporine treated a group of mice showed normoglycaemic status throughout the whole experimental period, but the cholesterol level remained significant (P<0.001) till the end of the experimental d. Gradually decrements in glycaemia of the diabetic pancreatectomised animals demonstrate islets neogenesis occurring after the operative activity, leading to normoglycaemic condition, probably attributed to β-cells proliferation.

Conclusion: The biochemical and histopathological evaluations suggest that there is the development of the diabetic model in the pancreatectomized group and diabetes status induced by pancreatectomy is curable to a certain extent due to the regeneration of β-cells.


Keywords


Diabetes, Partial pancreatectomy, Euglycaemic status, Nesidioblastotic activity, Glycaemia, Normoglycaemic status, β-cells proliferation

| HTML | PDF |

References


Chatzigeorgiou A, Halapas A, Kalafatakis K, Kamper E. The use of animal models in the study of diabetes mellitus. Int J Exp Clin Pathophysiol Drug Res 2009;23:245-8.

Jain R, Jain P, Jain P. A review on treatment and prevention of diabetes mellitus. Int J Curr Pharm Res 2016;8:16-8.

Kabi S, Dhar Kumar S, Panda Nath B, Das C, Jena Kant K, Sahu Chandra M. Surveillance of mortality: from a tertiary care teaching hospital of eastern India in persons with or without diabetes mellitus. Asian J Pharm Clin Res 2017;10:87-90.

Homsi AI MF, Lukic ML. An update on the pathogenesis of diabetes mellitus, department of pathology and medical microbiology (Immunology Unit) faculty of medicine and health sciences, UAE University. Al Ain United Arab Emirates 1992;3:460-78.

Poscoe WS, Jenkins AB, Kusunoki M, Storlein LH. Insulin action and determinants of glycaemia in a rat model of type2 diabetes mellitus. Diabetologia 1992;35:208-15.

Mandare PA, Deokar N, Patil VS, Gaikwad PB. Study of total cholesterol, triacylglycerols, high-density lipoprotein cholesterol in type 2 diabetes mellitus. Asian J Pharm Clin Res 2016;10:116-8.

Gupta A, Sharma M, Sharma J. A role of insulin in different types of diabetes: a review. Int J Curr Microbiol Appl Sci 2015;4:58-77.

Shaw JE, Sicreer RA, Zimmet PZ. Diabetes research and clinical practice. ELSEVIER 2010;87:4-14.

Jayatunge SP, Mahendra G, Siyabalapitiya SS, Siriwardana RC, Liyanage C. Total pancreatectomy for cholangiocarcinoma of the distal common bile duct associated with lipomatous pseudohypertrophy of the pancreas. Int J Hepatobilliary Pancreatic Diseases 2015;5:30-4.

Hardikar AA, Karandikar MS, Bhonde RR. Effect of partial pancreatectomy on diabetes status in BALB/C mice. J Endocrinol 1999;162:189-95.

Rajgopal G, Toora BD. Practical biochemistry. 2nd ed. Ahuja Publishing House; 2007.

Moron MS, Depierre JW, Mannervile B. Levels of glutathione, glutathione reductase and glutathione transferase activities in rat lung and liver. Biochem Biophysics Acta 1979;582:67-78.

Qadori Yasamin Thamer. Histological studies on pancreatic tissue in diabetic rats by using wild cherry. IRAQI Postgraduate Med J 2011;10:421-5.

Vaziri DN, Liang K, Azad H. Effect of cyclosporine on HMG-CoA reductase, cholesterol 7α-hydroxylase, LDL receptor, HDL receptor, VLDL receptor, and lipoprotein lipase expressions. J Pharmacol Exp Ther 2000;294:778-83.

Alenjandro Rodolfo, Cutfield Richard, Frances L, Latif Zuhair, Mint Z. Successful long-term survival of pancreatic islet al. log rafts in spontaneous or pancreatectomy induced diabetes in dogs: cyclosporine-induced unresponsiveness. John Wiley Sons Press; 1985;34:825-8.

Wolff SP, Dean RT. Glucose antioxidation and protein modification. The potential role of ‘autoxidative glycosylation’ in diabetes. Biochem J 1987;245:243-50.

Babizhayev MA, Lankin VZ, Savel Yeva EL, Deyev AL, Yegorov YE. Diabetes mellitus: novel insights, analysis and interpretation of pathophysiology and complications management with imidazole containing peptidomimetic antioxidants. Recent Patents Drug Delivery Formulations 2013;7:216-56.

Kasznicki J, Kosmalski M, Sliwinska A, Mrowicka M, Stancyzk M, Majsterek I, et al. Evaluation of oxidative stress markers in the pathogenesis of diabetic nephropathy. Mol Biol Reports 2012;39:8669-78.

Tiwari Kumar Brahm, Pandey Bhooshan Kanti, Abidi AB, Rizvi Ibrahim Syed. Markers of oxidative stress during diabetes mellitus: a review article. J Biomarkers 2013;378790:8. http://dx.doi.org/10.1155/2013/378790

Dias Vera, Junn Eunsung, Mouradian Maral M. The role of oxidative stress in Parkinson’s disease. J Parkinsons Dis 2013;3:461-91.




About this article

Title

PRE-CLINICAL STUDIES ON DIABETES MELLITUS USING PARTIAL PANCREATECTOMY IN SWISS ALBINO MICE

Keywords

Diabetes, Partial pancreatectomy, Euglycaemic status, Nesidioblastotic activity, Glycaemia, Normoglycaemic status, β-cells proliferation

DOI

10.22159/ijpps.2018v10i1.21250

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 1, 2018 Page: 71-76

Online ISSN

0975-1491

Authors & Affiliations

Shibu Narayan Jana
Department of Pharmaceutical Sciences and Technology, BIT, Mesra, Ranchi
India

Papiya Mitra Mazumder
Department of Pharmaceutical Sciences and Technology, BIT, Mesra, Ranchi
India


Article Tools



Refbacks

  • There are currently no refbacks.