• Abdelmohsen M. M. Phytochemistry Department, National Research Centre, 12311 Dokki, Cairo Egypt
  • Nazif N. M. Phytochemistry Department, National Research Centre, 12311 Dokki, Cairo Egypt
  • Seif El-Nasr M. M. Phytochemistry Department, National Research Centre, 12311 Dokki, Cairo Egypt


Objective: Echinacea purpurea is used widely for its unspecific enhancement of the immune system. It contains multiple bioactive substances, including, phenolics (caffeic acid derivatives), flavonoids, anthocyanins and alkamides. In this currently investigation, efforts are focused on the evaluation of extraction techniques viz., conventional extraction (CE) and microwave-assisted extraction (MAE) for the regeneration of the active constituents of Echinacea purpurea.

Methods: The aerial parts have been separately extracted with 80% methanol by two methods; CE and MAE. Identification of the isolated compounds was carried out by spectroscopic analysis viz., UV, TLC, 1H- NMR and MS. TLC scanner system and RP-HPLC with UV detection have been employed for the quantitative determination of caffeic acid derivatives and that of alkamides content in both extracts.

Results: Chicoric and caftaric acids have been isolated and identified. The results of the TLC scanner system have revealed that the concentration is about 1.19 ± 0.02 and 1.35 ± 0.03 % (w/w) for caftaric and chicoric acid, respectively of the dry plant material extracted with CE, while the concentration is 1.81 ± 0.04 and 6.19 ± 0.26 % (w/w) for the two acids, respectively of the dry plant material extracted by MAE. The results of the HPLC have revealed that the concentration of alkamides is 0.25 and 0.33 % (w/w) of the dry plant material obtained with CE and MAE, respectively.

Conclusion: Our study proved that MAE is a more effective technique compared to the CE. Extraction time was reduced, with the use of less solvent and the yield amount of the extracted active compounds was increased.

Keywords: Echinacea purpurea, Chicoric acid, Alkamides, HPLC, Microwave-assisted extraction.


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1. Rochfort S, Parker AJ, Dunshea FR, Plant bioactives for ruminant health and Productivity. Phytochemistry 2008;69:299–322.
2. Blumenthal M, Goldberg A, Brinkmann J, Herbal Medicine: Expanded Commission E Monographs. Boston (MA):Integrative Medicine Communications; 2000.
3. Baum BR, Binns SE, Arnason JT. Integrating recent knowledge about the genus Echinacea: morphology, molecular systematics, phytochemistry. Herbal Gram 2006;72:32–46.
4. Perry B, Burges E, Glennie V. Echinacea standardization: analytical methods for phenolic compounds and typical levels in medicinal species. J Agric Food Chem 2001;49:1702–06.
5. Goel V, Chang C, Slama JV, Barton R, R Bauer, Gahler R, Echinacea stimuales macrophage function in the lung and spleen of normal rats. J of Nutritional Biochemistry 2002;13:487-92.
6. Barret B. Medicinal properties of Echinacea: a critical review. Phytomedicine 2003;10:66-86.
7. Thygesen L, Thulin J, Mortensen A, Skibsted LH, Molaard P. Antioxidant activity of chicoric acid and alkamides from Echinacea purpurea, alone and in combination. Food Chemistry 2007;101:74-81.
8. Bergeron CS, Gafner LL, Batcha KL, Angerhofer L. Stabilization of caffeic acid derivatives in Echinacea purpurea L. glycerin extract. J Agricultural Food Chemistry 2002;5:3967-70.
9. Harborne JB, Williams CA. Phytochemistry of the genus Echinacea. In: Miller SC (Ed. ) Echinacea. The genus Echinacea. CRC. Boca Raton 2004;55–71.
10. Schulthess BH, Giger E, Baumann TW. Echinacea: anatomy, phytochemical pattern, and germination of the achene. Planta Medica 1991;57:384–8.
11. He XG, Lin LZ, Bernart M W, Lian L Z. Analysis of alkamides in roots and achences of Echinacea purpurea by liquid chromatography-electrospray mass spectrometry. Chromatography A 1998;815:205-11.
12. Bryngelsson S, Dimberg LH, Kamal-Eldin A. Effects of commercial processing on levels of antioxidants in oats (Avena sativa L. ). J of Agricultural and Food Chemistry 2002;50:1890-6.
13. Stevenson DG, Inglett GE, Chen D, Biswas A, Eller FJ, Evangelista RL. Phenolic content and antioxidant capacity of supercritical carbon dioxide-treated and air-classified oat bran concentrate microwave-irradiated in water or ethanol at varying temperatures. Food Chemistry 2008;108:23-30.
14. Liazid A, Guerrero RF, Cantos E, Palma M, Barroso CG. Microwave assisted extraction of anthocyanins from grape skins. Food Chemistry 2011;124:1238-43.
15. Fang X, Wang J, Zhou H, Jiang X, Zhu L, Gao X. Microwave-assisted extraction with water for fast extraction and simultaneous RP-HPLC determination of phenolic acids in Radix Salviae miltiorrhizae. J Sep Sci 2009;32:2455-61.
16. The European Pharmacopoeia Forum, Pharmeuropa 2004;16:545.
17. United States Pharmacopoeia (USP) 2005;28-NF 23.
18. Hassanein HD, Said-AL Ahl Hah, AbdelMohsen MM. Antioxidant polyphenolic constituents of Satureja montana L. growing in Egypt. Int J of Pharmacy and Pharm Sci 2014;6:578-81.
19. Bauer R, Remiger P. TLC and HPLC analysis of alkamides in Echinacea drugs. Planta Medica 1989;55:367-71.
20. Clifford LJ, Nair MG, Rana J, Dewitt DL. Bioactivity of alkamides isolated from Echinacea purpurea (L. ) Moench. Phytomedicine 2002;9:249-53.
21. Sun J, Liang F, Bin Y, Li P, Duan C. Screening non-colored phenolics in red wines using liquid chromatography/ultraviolet and mass spectrometry/ mass spectrometry libraries. Molecules 2007;12:679-93.
22. Cheminat A, Zawatzky R, Becker H, Brouillard R. Caffeolyl conjugates from Echinacea species: structures and biological activity. Phytochemistry 1988;27:2787-94.
23. Zolgharnein J, Niazi A, Afiuni-Zadeh S, Zamani K. Determination of Cichoric acid as a biomarker in Echinacea purpurea cultivated in Iran using high performance liquid chromatography. Chinese Medicine 2010;1:23-7.
24. Olah NK, Radu L, Mogasan C, Hangaru D, Gocan S. Phytochemical and pharmacological studies on Orthosiphon stamineus Benth. (Lamiaceae) hydroalcoholic extracts. J of Pharm and Biomedical Analysis 2003;33:117-23.
25. Proestos C, Komaitis M. Application of microwave-assisted extraction to the fast extraction of plant phenolic compounds. LWT-Food Sci Technology 2008;41:652-9.
26. Sutivisedsak N, Cheng HN, Willett JL, Lesch WC, Tangsrud RR, Biswas A. Microwave-assisted extraction of phenolics from bean (Phaseolus vulgaris L. ). Food Res Int 2010;43:516-9.
27. Routray W, Orsat V. MAE of phenolic compounds from blueberry leaves and comparison with extraction methods. Industrial Crops and Products 2014;58:36-45.
28. Schulz V, Hansel R, Tyler VE. Rational Phytotherapy; A Physcians Guide to Herbal Medicine, third ed. , Springer-Verlag, Berlin, Heidelberg: New York: 1998. p. 273-8.
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How to Cite
M., A. M., N. N. M., and S. E.-N. M. M. “MICROWAVE-ASSISTED EXTRACTION OF BIO-ACTIVE COMPOUNDS (PHENOLICS AND ALKAMIDES) FROM ECHINACEA PURPUREA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 265-8, https://innovareacademics.in/journals/index.php/ijpps/article/view/2220.
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