ASSESSING THE THERAPEUTIC ROLE OF JOSHANDA: PHYTOCHEMICAL, ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIMICROBIAL ACTIVITIES
Objective: Joshanda, a polyherbal Unani formulation is extensively used as a common home remedy for the treatment of a cough and cold accompanied by pharyngeal inflammation and fever. This study aimed to analyze phytochemicals, antioxidant, anti-inflammatory, and antibacterial activity.
Methods: The study investigated the presence of phyto-compounds in joshanda and antioxidant, antibacterial, anti-fungal, and anti-inflammatory activities by various in vitro standard methods using ascorbic acid, ampicillin, and aspirin respectively as standard drugs.
Results: Joshanda aqueous extract revealed the presence of tannins, phenols, flavonoids glycosides, terpenoids, and alkaloids and absence of sterols, saponins, xanthoprotiens, and carboxylic acid. Joshanda showed the highest inhibition against B. subtilis (% MGI 99.000Â±0.577) and least inhibition against P. aeruginosa (%MGI of 84.102Â±0.491). Joshanda extract, ascorbic acid demonstrated highest % DPPH radical scavenging of 98.379Â±0.313%, 98.843Â±0.443% and a minimum of 36.210Â±1.174%, 83.192Â±0.422%. Results showed H2O2 scavenging activity of 0.047Â±0.001 Î¼g/ml per minute degradation of H2O2. FRAP value was observed in joshanda and ascorbic acid with a maximum of 0.945Â±0.024, 0.687Â±0.047 mmol and minimum of 0.171Â±0.036, 0.059Â±0.005 mmol respectively. Joshanda extract showed the highest albumin denaturation inhibition of 14.069Â±0.350% and the lowest of 1.880Â±0.194% at extract volume of 1000 Âµl and 100 Âµl respectively. The extract demonstrated the highest proteinase inhibition of 24.003Â±0.291 % and the lowest of 4.959Â±0.254% comparable to aspirin. Joshanda had no potent anticandidal activity up to 1 mg/ml.
Conclusion: Results clearly suggested that joshanda is a potent phytodrug and can also be used as a strong reactive oxygen species scavenger, might be used as anti-arthritic and strong natural antibiotic agent for effective treatment of various oxidative stressed disorders.
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