• Bo-ram Yeon Department of Biological Environment, Kangwon National University, Chuncheon 200-701, Republic of Korea.
  • Songmun Kim Department of Biological Environment, Kangwon National University, Chuncheon 200701, Republic of Korea
  • Kandhasamy Sowndhararajan Department of Biological Environment, Kangwon National University, Chuncheon 200701, Republic of Korea
  • Ji-wook Jung 2Department of Herbal Medicinal Pharmacology, Daegu Haany University, Kyungsan, Republic of Korea,
  • Jin-woo Jhoo Department of Animal Products and Food Science, Kangwon National University, Chuncheon 200701, Republic of Korea.


Objective: The main purpose of this study was to evaluate the volatile composition of supercritical fluid extract (SFE) obtained from Cnidium officinale Makino rhizomes.

Methods: GC/MS analyses were carried out with the direct- and solid phase microextraction (SPME) of SFE obtained from rhizomes. In addition, GC/MS analysis was performed for the rhizomes of C. officinale using SPME.

Results: SPME-GC/MS analysis of the rhizomes revealed the separation of 23 components. Among these, β-phellandrene (20.38%), dictyotene (12.98%), β-pinene (10.59%), β-selinene (9.45%), eugenol (7.71%) and β-farnesene (7.09%) were found to be major components. In the SFE analyzed by direct-GC/MS, linoleic acid (19.26%), 2-methoxy-4-vinylphenol (18.98%), hexadecanoic acid (12.15%), and β-selinene (9.44%) were identified as major components. Whereas, 3,4-dihydrocoumarin (16.94%), shyobunone (14.07%), dictyotene (10.65%), p-cresol (10.17%), zierone (6.36%) and umbellulone (5.71%) were major components in the SFE analyzed by SPME-GC/MS.

Conclusion: The present study clearly suggested that the SPME-GC/MS analysis of SFE provided the separation of more number with diverse groups of compounds than the direct-GC/MS.


Keywords: Cnidium officinale, Gas chromatography, Rhizome, Solid phase microextraction, Supercritical carbon dioxide extract


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Yeon, B.- ram, S. Kim, K. Sowndhararajan, J.- wook Jung, and J.- woo Jhoo. “COMPARISON OF VOLATILE COMPOSITION OF SUPERCRITICAL CARBON DIOXIDE EXTRACT FROM RHIZOMES OF KOREAN MEDICINAL PLANT ‘CHUN-KUNG’ (CNIDIUM OFFICINALE MAKINO) BY DIRECT-AND SPME-GC/MS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 355-8,
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