• Vijay Kant Pandey Department of Bio-Engineering, Birla Institute of Technology, Ranchi, India.
  • Rashmi Mishra Department of Bio-Engineering, Birla Institute of Technology, Ranchi, India.
  • Ramesh Chandra Department of Bio-Engineering, Birla Institute of Technology, Ranchi, India.


Objective: The purpose of the present investigation was to establish in vitro conditions for moss Bryum coronatum Schwaegr. And to carry out preliminary phytochemical screening of B. coronatum leaf extracts in different solvents.

Methods: Fresh unopened, mature capsules were used as explant and surface sterilization of spores bearing capsule with different concentration of sodium hypochlorite (0.5, 1, 2, 4 and 8%) with different time duration. The Mu­rashige and Skoog (MS) medium that contains different concentration(full,1/2, 1/4th, 1/8th strength) with different concentration of sucrose were used for culture this moss. Phytochemical screening were carried out using ethanol, methanol and ethyl acetate leaf extract of B. coronatum to identify various constitutes using the standard procedures.

Results: Surface sterilization of spores of this moss was most effective in 4% commercial bleach for 1 min sterilization. The optimum condition for germination of spores and for proper growth of gametophytes B. coronatum on MS/4 medium strength with sucrose (1.5%), at pH 5.8 and temperature 22±2ºC with 16/8h: light/dark condition. Phytochemical analyses revealed the presence of alkaloids, terpenoids and saponins in all extracts.

Conclusion: Four percentage NaOCl aqueous solutions are better for surface sterilization of moss sporophytes. MS/4 medium with 1.5% sucrose found the best medium for spore germination. Solvents extracts showed presence of alkaloids, terpenoids and saponins in all extracts.

Keywords: In vitro, Bryum coronatum, Spores, Alkaloids, Terpenoids, Saponins.


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Author Biography

Ramesh Chandra, Department of Bio-Engineering, Birla Institute of Technology, Ranchi, India.
Department of Bio-Engineering, Associate Professor


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How to Cite
Pandey, V. K., R. Mishra, and R. Chandra. “IN VITRO CULTURE OF MOSS BRYUM CORONATUM SCHWAEGR.(BRYACEAE) AND IT’S PHYTOCHEMICAL ANALYSIS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, Sept. 2014, pp. 307-11,
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