IN VITRO CULTURE OF MOSS BRYUM CORONATUM SCHWAEGR.(BRYACEAE) AND IT'S PHYTOCHEMICAL ANALYSIS
Keywords:
In vitro, Bryum coronatum, Spores, Alkaloids, Terpenoids, SaponinsAbstract
Objective: The purpose of the present investigation was to establish in vitro conditions for moss Bryum coronatum Schwaegr. And to carry out preliminary phytochemical screening of B. coronatum leaf extracts in different solvents.
Methods: Fresh unopened, mature capsules were used as explant and surface sterilization of spores bearing capsule with different concentration of sodium hypochlorite (0.5, 1, 2, 4 and 8%) with different time duration. The MuÂrashige and Skoog (MS) medium that contains different concentration(full,1/2, 1/4th, 1/8th strength) with different concentration of sucrose were used for culture this moss. Phytochemical screening were carried out using ethanol, methanol and ethyl acetate leaf extract of B. coronatum to identify various constitutes using the standard procedures.
Results: Surface sterilization of spores of this moss was most effective in 4% commercial bleach for 1 min sterilization. The optimum condition for germination of spores and for proper growth of gametophytes B. coronatum on MS/4 medium strength with sucrose (1.5%), at pH 5.8 and temperature 22±2ºC with 16/8h: light/dark condition. Phytochemical analyses revealed the presence of alkaloids, terpenoids and saponins in all extracts.
Conclusion: Four percentage NaOCl aqueous solutions are better for surface sterilization of moss sporophytes. MS/4 medium with 1.5% sucrose found the best medium for spore germination. Solvents extracts showed presence of alkaloids, terpenoids and saponins in all extracts.
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References
Hallingback T, Hodgetts N. Status survey and conservation Action plan for Bryophytes: mosses, liverworts and hornworts. IUCN/SSC Bryophyte Specialist Group. IUCN, Gland, Switzerland and Cambridge, UK; 2000. p. X+106.
Kenrick P, Crane PR. The origin and early evolution of plants on land. Nature 1997;389:33â€9.
Graham LE, Cook ME, Busse JS. The origin of plants: body plan changes contributing to a major evolutionary radiation. Proc Natl Acad Sci USA 2000;97:4535â€40.
Taylor TN, Kerp H, Hass H. Life history biology of early land plants: deciphering the gametophyte phase. Proc Natl Acad Sci USA 2005;102:5892â€97.
Glime JM. Methods in Bryology. Mainz: Proc Bryol Meth. Workshop 1988;1-16:99-105.
Vanderpoorten A, Goffinet B. Introduction to bryophytes. Cambridge: University Press; 2009. p. 26-42, 185-213.
Reski R. Development, genetics and molecular biology of mosses. Bot Acta 1998;111:1â€15.
Reski R. Physcomitrella patens as a novel tool for plant functional genomics. In: Vasil IK. (ed) Plant biotechnology 2002 and beyond. Kluwer Acad Publ; 2003. p. 205â€9.
Cove DJ, Bezanilla M, Harries P, Quatrano RS. Mosses as model systems for the study of metabolism and development. Annu Rev of Plant Biol 2006;57:497â€520.
Beike AK, Decker EL, Frank W, Lang D, Vervliet-Scheebaum M, Zimmer AD, Reski R. Applied Bryology Botechnology. Trop Bryol 2010;31:22â€32.
Prigge MJ, Bezanilla M. Evolutionary crossroads in developmental biology: physcomitrella patens. Development 2010;137:3535â€43.
Reski R, Abel WO. Induction of budding on chloronemata and caulonemata of the moss, Physcomitrella patens, using isoâ€pentenyladenine. Planta 1985;165:354â€58.
Decker EL, Reski R. The moss bioreactor. Curr Opin Plant Biol 2004;7:166â€70.
Hohe A, Reski R. From axenic spore germination to molecular farming: one century of bryophyte in vitro culture. Plant Cell Rep 2005;23:513â€21.
Sabovljevic A, Sabovljevic M. Bryophytes, a source of bioactive and new compounds, in Recent Progress in Medicinal Plants Vol. 22:Phytopharmacology and Therapeutic values IV, Studium Press LLC, TX; 2008. p. 9-25.
Sabovljevic A, Sokovic M, Sabovljevic M, Grubisic D. Antimicrobial activity of Bryum argenteum. Fitoterapia 2006;77:144-45.
Krishnaiah D, Sarbatly R, Bono A. Phytochemical antioxidants for health and medicine: A move towards nature. Biotechnol Mol Biol Rev 2007;1:97-104.
Mahato SB, Sen S. Advances in triterpenoid research, 1990-1994. Phytochemistry 1997;44:1185-236.
Kappers IF, Aharoni A, Van Herpen TW, Luckerhoff LL, Dicke M, et al. Genetic engineering of terpenoid metabolism attracts bodyguards to Arabidopsis. Sci 2005;309:2070-72.
Herouart D, Sangwan RS, Fliniaux MA, Sangwan-Norreel BS. Variations in the Leaf Alkaloid Content of Androgenic Diploid Plants of Datura innoxia. Planta Med 1988;54:14-7.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 1962;15:473.
Sabovljevic M, Bijelovic A, Dragicevic I. In vitro culture of mosses: Aloina aloides (K. F. Schultz) Kindb, Brachythecium velutinum (Hedw. ) B. S. &G. , Ceratodon purpureus (Hedw. ) Brid. , Eurhynchium praelongum (Hedw. ) B. S. &G. and Grimmia pulvinata (Hedw. ) Sm. Turk J Bot 2003;27:441-46.
Vujicic M, Sabovljevic A, Sabovljevic M. Axenically culturing the bryophytes: a case study of the moss Dicranum scoparium Hedw. (Dicranaceae, Bryophyta), Botanica Serbica 2009;33(2):137-40.
Vujicic M, Cvetic T, Sabovljevic, Sabovljevic M. Axenically culturing the bryophytes: a case study of the liverwort Marchantia polymorpha L. ssp. Ruderalis Bischl. & Boisselier (Marchantiophyta, Marchantiaceae). Kragujevac J Sci 2010;32:73-81.
Sabovljevic A, Sabovljevic M, Grubisic D. Giberellin influence on the morphogenesis of the moss Bryum argenteum Hedw. in vitro conditions. Arch Biol Sci 2010;62(2):373-80.
Chen YY, Lou YX, Guo SL, Cao T. Successful tissue culture of the medicinal moss Rhodobryum giganteum and factor influencing proliferation of its protonemata. Ann Bot Fennici 2009;46:516-24.
Schween G, Hohe A, Koprivova A, Reski R. Effects of nutrients, cell Density, culture techniques on protoplast regeneration and early protonema development in moss, Physcomitrella patens. J Plant Physiol 2003;160:209-12.
Welander T. Effects of nitrogen, sucrose, IAA and kinetin on Beta vulgaris grown in vitro. Physiol Plant 1976;36:7-10.
Custodio L, Martins-Loucao MA, Romano A. Influence of sugars on in vitrorooting and acclimatization of carob tree. Biol Plant 2004;48:469-72.
Vasudevan A, Selvaraj N, Ganapathi A, Kasthurirengan S, Ramesh Anbazhagan V, Manickavasagam M. Glutamine: a suitable nitrogen source for enhanced shoot multiplication in Cucumis sativus L. Biol Plant 2004;48:125-28.
Mehta P, Chopra RN. The effect of some chemical factors on growth and fertility in male clones of the moss Microdus brasiliensis. Cryptog Bryol Lichen 1991;12:55-62.
Sarla. In vitro studies on male clone of Riccia discolor: effect of sugars. J Hattori Bot Lab 1992;72:141-50.
Kumar P, Chopra RN. Effect of some physical factors on growth and gametangial induction in male clones of the three mosss grown in vitro. Bot Gaz 1983;144(4):533-39.
Pandey M, Debnath M, et al. Phytomedicine: an ancient approach turning into future potential source of therapeutics. J Pharmacognosy Phytother 2011;3(1):113-17.
Victor Njoku O, Chidi O. Phytochemical constituents of some selected medicinal plants, Afr J Pure and Appl Chem 2009;3(11):228-33.
Sofowora AA. Medicinal Plants and Traditional Medicine in Africa, 2nd Ed. Spectrum Books Ltd: Iba-dan; 1993. p. 81-5.
Cowman MM. Plant Products as Antimicrobial Agents. Clinic Microbiol Rev 1999;12(4):564-82.
Edeoga HO, Eriata DO. Alkaloids, Tannin and Saponin Contents of Some Nigerian Medicinal Plants. J Med Aro Plant Sci 2001;23:344-49.