FORMULATION AND EVALUATION OF PECTIN-CALCIUM CHLORIDE BEADS OF AZATHIOPRINE FOR COLON TARGETED DRUG DELIVERY SYSTEM


Samir A Atara, Moinuddin M Soniwala

Abstract


Objective: Calcium pectinate is an insoluble hydrophilic material used for sustained release delivery. The objective of the study was formulation and evaluation pectin-calcium chloride beads of azathioprine.

Methods: Calibration curves of azathioprine were prepared in 0.1N HCl solution (pH-1.2) and phosphate buffer (pH-6.8 and pH-7.4). Fourier transform infrared spectroscopy and differential scanning calorimetry was used to determine compatibility between azathioprine and excipients. Formulation and optimization of calcium-pectinate beads were performed. The coating of the optimized batch was performed with eudragit S100. Micrometric properties, scanning electron microscopy, in vitro azathioprine release, and stability study was performed. Dissolution kinetic study was assessed for various kinetics models of the optimized batch. The Wilcoxon test followed by the Dunnett’s multiple comparison tests were performed between uncoated and coated beads in vitro dissolution profile of optimized batch in 0.1N HCl after 2 h.

Results: Fourier transform infrared spectroscopy and differential scanning calorimetry had identical peaks with that of pure azathioprine in compatibility study. Scanning electron microscopy of an uncoated optimized batch of beads indicated the smooth and uniform surface of prepared beads. Eudragit S100 coating was decreased release of azathioprine release in 0.1N HCl after 2 h (p = 0.049, q = 3.533). Korsmeyer/Peppa's model was applied with release exponent higher than 1. In vitro percentage cumulative azathioprine release was identical before and after stability study.

Conclusion: Calcium pectinate beads of azathioprine, a multi-particulate dosage form using pH-dependent approaches were prepared.


Keywords


Azathioprine, Bead, Calcium carbonate, Eudragit S100, Pectin

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About this article

Title

FORMULATION AND EVALUATION OF PECTIN-CALCIUM CHLORIDE BEADS OF AZATHIOPRINE FOR COLON TARGETED DRUG DELIVERY SYSTEM

Keywords

Azathioprine, Bead, Calcium carbonate, Eudragit S100, Pectin

DOI

10.22159/ijpps.2018v10i1.23175

Date

01-01-2018

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 10, Issue 1, 2018 Page: 172-177

Online ISSN

0975-1491

Authors & Affiliations

Samir A Atara
School of Pharmacy, RK University
India

Moinuddin M Soniwala
BK Mody Government Pharmacy college
India


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