A WEB-BASED RESOURCE FOR STRUCTURAL INFORMATION ON eIF5A AND ITS RELATED PROTEINS: NEW POTENTIAL THERAPEUTIC TARGETS IN MANY HUMAN DISORDERS
Keywords:
eIF5A, Hypusine, Deoxyhypusine synthase, Elongation factor P, Structural database, Potential therapeutic targetsAbstract
Objective: There is a considerable therapeutic interest in eIF5A as a potential target for drug development through inhibition of hypusination. In this regards, protein structural information is fundamental. Herein, we reported the developing of a web-based system, called eIF5ADB, which compiles all protein structural data on eIF5A and its related proteins.
Methods: The eIF5ADB database was implemented as a MySQL relational database, using PHP scripting language. Web interfaces were developed using HTML, CSS and JavaScript. The data were collected from PDB, UniProt and Entrez databases. These data were filtered appropriately using specialized literature.
Results: The database provides three modules that allow to search, acquisition of contents and access to statistical data, besides direct links to matching to external databases.
Conclusion: The platform developed here is very useful for researchers interested in this content and can be accessed at http: //www. gurupi. uft. edu. br/btoxdb/eif5adb.
Â
Downloads
References
Park MH, Nishimura K, Zanelli CF, Valentini SR. Functional significance of eIF5A and its hypusine modification in eukaryotes. Amino Acids 2010;38(2):491-500.
Rossi D, Kuroshu R, Zanelli CF, Valentini SR. eIF5A and EF-P: two unique translation factors are now traveling the same road. Wiley Interdiscip Rev RNA 2014;5(2):209-22.
Dever TE, Gutierrez E, Shin BS. The hypusine-containing translation factor eIF5A. Crit Rev Biochem Mol Biol 2014;17:1-13.
Glick BR, Ganoza MC. Identification of a soluble protein that stimulates peptide bond synthesis. Proc Natl Acad Sci USA 1975;72:4257–60.
Ude S, Lassak J, Starosta AL, Kraxenberger T, Wilson DN, Jung K. Translation elongation factor EF-P alleviates ribosome stalling at polyproline stretches. Sci 2013;339(6115):82-5.
Zou SB, Roy H, Ibba M, Navarre WW. Elongation factor P mediates a novel post-transcriptional regulatory pathway critical for bacterial virulence. Virulence 2011;2(2):147-51.
Wang F, Guan X, Xie D. Roles of Eukaryotic Initiation Factor 5A2 in Human Cancer. Int J Biol Sci 2013;9(10):1013–20.
Kaiser A. Translational control of eIF5A in various diseases. Amino Acids 2012;42(2-3):679-84.
Sun Z, Cheng Z, Taylor CA, McConkey BJ, Thompson JE. Apoptosis induction by eIF5A1 involves activation of the intrinsic mitochondrial pathway. J Cell Physiol 2010;223:798-809.
Taylor CA, Sun Z, Cliche DO, Ming H, Eshaque B, Jin S, et al. Eukaryotic translation initiation factor 5A induces apoptosis in colon cancer cells and associates with the nucleus in response to tumour necrosis factor alpha signalling. Exp Cell Res 2007;313:437-49.
Hoque M, Hanauske-Abel HM, Palumbo P, Saxena D, D'Alliessi Gandolfi D, Park MH, et al. Inhibition of HIV-1 gene expression by Ciclopirox and Deferiprone, drugs that prevent hypusination of eukaryotic initiation factor 5A. Retrovirology 2009;6:90.
De Almeida OPJr, Toledo TR, Rossi D, Rossetto DdeB, Watanabe TF, Galvão FC, et al. Hypusine modification of the ribosome-binding protein eIF5A, a target for new anti-inflammatory drugs: understanding the action of the inhibitor GC7 on a murine macrophage cell line. Curr Pharm Des 2014;20(2):284-92.
Maier B, Tersey SA, Mirmira RG. Hypusine: a new target for therapeutic intervention in diabetic inflammation. Discov Med 2010;10(50):18-23.