28-HOMOCASTASTERONE: A NOVEL DIETARY PHYTO KETO OXYSTEROL MODULATING TESTICULAR STEROID METABOLISM AND LxR mRNA EXPRESSION IN DIABETIC RAT
Objective: Present study aims to investigate 28-homocastasterone (28-HC) influences on testicular tissue in the normal and diabetic rat.
Methods: Induction of diabetes was achieved by single peritoneal injection of streptozotocin (60 mg/kg b. wt) followed by 28-HC (100 Âµg/150 gm body weight) administration by oral gavage for 15 consecutive days to experimental rats. 3Î²-hydroxysteroid dehydrogenase, 17Î²-hydroxysteroid dehydrogenase activities, testosterone level, Liver X Receptor (LxR) mRNA expression, malondialdehyde (MDA), reduced glutathione (GSH) and testis histology was analysed.
Results: Increased cholesterol level, 3Î²-hydroxysteroid dehydrogenase (3Î²HSD), 17Î²-hydroxysteroid dehydrogenase (17Î²HSD) activities, testosterone level with significant elevation of LxR-Î± and Î² mRNA expression in treated rat testis. A significant reduction found inMDA and increased reducedGSH along with improved testicular architecture was observed.
Conclusion: In the present study demonstrated that 28-HC induced 3Î²HSD, 17Î²HSD enzyme activity and testosterone level, thus indicative of steroidogenic potential and capable of transactivating LxR-Î± and Î² molecular operative in rat testicular tissue.
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