ANTIOXIDANT, PHYTOCHEMICAL AND ANTIBACTERIAL ACTION OF HIMALAYAN MEDICINAL HERBS PERISTROPHE BICALYCULATA LEAVES EXTRACT AGAINST RESPIRATORY TRACT PATHOGENS
Keywords:Antibacterial activity, Agar well diffusion method, Twofold serial dilution method, Antioxidant, Phytomedicine, Respiratory tract pathogens
Objective: Evaluate the antioxidant, phytochemicals and antibacterial screening of leaves extracts ofÂ Peristrophe bicalyculata.
Methods: The antibacterial activity against theÂ respiratory tract pathogens i.e., Staphylococcus aureusÂ (MTCC 1144),Â Streptococcus pneumoniaeÂ (MTCC 655),Â Streptococcus pyogenesÂ (MTCC 442), Pseudomonas aeruginosaÂ (MTCC 2474) and Klebsiella pneumoniae (MTCC 4030) was examined by the method agar well diffusion and the minimum inhibitory concentration (MICs) was determined by the method of twofold serial dilution. Broad spectrum antibiotic erythromycin was used as positive control and dimethyl sulphoxide (DMSO) used as negative control. The qualitative method was adapted for phytoconstituents screening and antioxidant activity of plant extract was examined by DPPH free radical scavenging method.
Results: The results showed that the chloroform (CHF) extract has a higher degree of antibacterial potency then the other extract. The zone of inhibition showed by chloroform extract against tested bacteria ranged between 9.3Â±0.59 mm to 26.6Â±0.66 mm, respectively. MICs values were recorded between 6.25 mg/ml to 25Â mg/ml against all the test organisms. Phytoconstituents analysis of P. bicalyculata extract exposed the presence of alkaloids, flavonoids, glycosides, steroids, saponins and tannins. The methanolic extract of P. bicalyculata % inhibition of DPPH radical is up to 86.33%. The P. bicalyculata (Methanolic extract) gives best antioxidant activity than another extract.
Conclusion: This investigation ropes a good answer to the use ofÂ P. bicalyculataÂ as a natural antioxidant and in herbal medicine as a support for the development of new drugs and phytomedicine in the foundation for its use in remedial of respiratory infectious diseases.
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