OXYCAROTENOID LUTEIN REVERSES THE TOXICITY INDUCED BY CARBOFURAN IN WISTAR RATS

  • Binitha P Purushothaman Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur 680 555, Kerala, India
  • Gayathri Valsan Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur 680 555, Kerala, India
  • Ramadasan Kuttan Department of Biochemistry, Amala Cancer Research Centre, Amala Nagar, Thrissur 680 555, Kerala, India

Abstract

Objective: Elucidation of the protective effect of lutein against carbofuran induced toxicity in Wistar rats.

Methods: Male Wistar rats were assigned into 5 groups of five animals. Group I normal received sunflower oil, Group 2 received carbofuran (5 mg/kg b. w.) alone. Group 3-5 received carbofuran plus lutein (50, 100 and 200 mg/kg body weight) respectively. Carbofuran and lutein administration were continued for 14 d. Neurobehavioural markers such as rotarod, grip strength test and pain threshold tests were carried out. After sacrifice, tissues were analysed for marker enzymes, antioxidant enzymes as well as oxidative stress markers.

Results: Low dose of carbofuran was found to produce neurobehavioral problems as seen from the decreased retention time during rotarod test, endurance capacity in grip strength test and increased endurance capacity in pain threshold test. They were found to be significantly reversed by oral lutein administration. Administration of lutein restored the decreased acetylcholinesterase produced by carbofuran. Serum and tissue marker enzymes such as lactate dehydrogenase, creatine kinase and gamma-glutamyltransferase, which were increased by carbofuran were decreased by lutein administration. Lutein administration also reduced oxidative stress parameters which were increased by carbofuran.

Conclusion: The results showed that carbofuran induced toxicity in male Wistar rats was reversed by carotenoid lutein.

Keywords: Carbofuran, Lutein, Acetylcholinesterase, Antioxidant enzymes, Oxidative stress

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Purushothaman, B. P., G. Valsan, and R. Kuttan. “OXYCAROTENOID LUTEIN REVERSES THE TOXICITY INDUCED BY CARBOFURAN IN WISTAR RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 7, July 2018, pp. 10-15, doi:10.22159/ijpps.2018v10i7.24446.
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