HPTLC METHOD FOR THE DETERMINATION OF LUPEOL FROM ANDROGRAPHIS ECHIOIDES LEAVES

  • S. Gurupriya Department of Chemistry, Holy Cross College, Tiruchirappalli 620002, TamilNadu, India
  • L. Cathrine Department of Chemistry, Holy Cross College, Tiruchirappalli 620002, TamilNadu, India
  • P. Pratheema Department of Chemistry, Holy Cross College, Tiruchirappalli 620002, TamilNadu, India

Abstract

Objective: A new simple, precise, rapid and selective highâ€performance thinâ€layer chromatographic (HPTLC) method has been developed for the determination of lupeol in methanolic leaves extract of Andrographis echioides.

Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of lupeol as standard on HPTLC plates for the quantification of lupeol from the Andrographis echioides leaves. The concentration of standard lupeol was 1 mg/ml.

Results: The retention factor of lupeol was found to be 0.55. Linearity was obtained in the range of 5000 ngâ€10000 ng for lupeol. The developed and validated HPTLC method was employed for lupeol in methanolic leaves extract of Andrographis echioides for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99917 for lupeol. Satisfactory recoveries of 99.80 % were obtained for lupeol.

Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of lupeol in methanolic leaves extract of Andrographis echioides.

Keywords: Andrographis echioides, Lupeol, HPTLC (high‐performance thin‐layer chromatographic) technique

Downloads

Download data is not yet available.

References

1. Dhalwal K, Shinde VM, Mahadik KR, Kakasaheb RM. Optimization and validation of reverse phase HPLC and HPTLC method for simultaneous quantification of vasicine and vasicinone in Sida species. Med Plants Res 2010;4 Suppl 13:1289-96.
2. Di X, Chan KKC, Leung HW, Huie CW. Fingerprint profiling of acid hydrolyzates of polysaccharides extracted from the fruiting bodies and spores of Lingzhi by high--performance thin-layer chromatography. J Chromatogr A 2003;1018:85-9.
3. Larsen T, Axelsen J, Ravn HW. Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin layer chromatography. J Chromatogr A 2004;1026:301-4.
4. Suthar AC, Banavaliker MM, Biyani MK, Priyadarsini Indira K, Sudarsan V, Mohan HA. High-performance thin layer chromatography method for quantitative estimation of lupeol in Crataeva nurvala. Indian Drugs 2001;38 Suppl 9:474-8.
5. Hanson JR. The biosynthesis of secondary metabolites. In Natural Products, the secondary Metabolites; 2003. p. 112-21.
6. Gershenzon J, Kreis W. Biosynthesis of monoterpenes, sesquiterpenes, diterpenes, sterols, cardiac glycosides and steroid saponins. In Biochemistry of Plant Secondary Metabolites. Annual Plant Review; 1999. p. 222-99.
7. Ruzicka L. The isoprene rule and the biogenesis of terpenic compounds. Experimentia 1953;9:357-67.
8. Gershenzon J, Dudareva N. The function of terpene natural products in the natural world. Nat Chem Biol 2007;3:408-14.
9. Patocka J. Biologically active pentacyclic triterpenes and their current medicine signification. J Appl Biomed 2003;1:7-12.
10. Subha dhirasakul S, Takayama H, Kitajima FM, Aimi NF. Triterpenoids from thai medicinal plant. Willughbeia firma. Natural Med 2000;54:155-7.
11. Ankita K, Handique A. Brief overview on Andrographis Paniculata (Burm. f) Nees, A High valued medicinal plant: boon over synthetic drugs. Asian J Sci Technol 2010;6:113-8.
12. Shanker AS, Lalit Kumar Tyagi, Mahendra S, Ch V Ra. Herbal medicine for market potential in india: an overview. Academic J Plant Sci 2008 1 Suppl 2:26-36.
13. Kirtikar. In: Indian Medicinal Plants. 3. New Delhi: Periodical Experts; 2007.
14. Zulfkar LQ, Beena J, Anandan R, Mohammed RU. Antibacterial activity of ethanol extracts of Indoneesiella echioides evaluated by the filter paper disc method. Pak J Pharm Sci 2009;22:123-5.
15. Kanchana N, Rubalakshmi. Phytochemical screening and antimicrobial activity of Andrographis echioides(L.) Nees–an indigenous medicinal plant. World J Pharm Pharm Sci 2014;3 Suppl 5:702-10.
16. Jayaprakasam D, Gunasekara B, Bodo. Dihydroechioidinin, aavanone from Andrographis echioides. Phytochemistry 1999;1 Suppl 3:92-7.
17. Nirubama K, Rubalakshmi. Bioactive compounds in Andrographis echioides (L.) Nees. leaves by GC-MS analysis. Int J Curr Res Biosci Plant Biol 2014;1 Suppl 3:92-7.
18. Deepti R, Sushila R, Permender R, Aakash D, Sheetal A, Dharmender R. HPTLC densitometric quantification of stigmasterol and lupeol from Ficus religiosa. Arab J Chem 2015; 8:366–71.
19. Suthar AC, Banavaliker MM, Biyani MK, Priyadarsini Indira K, Sudarsan V, Mohan HA. High-performance thin layer chromatography method for quantitative estimation of lupeol in Crataeva nurvala. Indian Drugs 2001;38 Suppl 9:474–8.
20. Badami S, Gupta MK, Ramaswamy S, Rai SR, Nanjaian M, Bendell DJ, Subban R, et al. Determination of betulin in Grewia titiaefolia by HPTLC. J Separ Sci 2004;27:129–31.
21. Purnima D, Hamrapurkar PK. HPTLC determination of stigmasterol and tocopherol acetate in Leptadenia reticulata and its formulation. J Plan Chromatogr 2007;20 Suppl 3:183–7.
22. Geneva. ICH, Q2A(R1), Validation of Analytical Procedure: Text and Methodology, International Conference on Harmonization Specification, November; 2005. p. 5–10.
23. Ravindra CS, Sanjay BK, Kalaichelvan VK. Phytochemical profile studies on the steroids of methanolic leaf extract of medicinally important plant Holoptelea integrifolia (Roxb.) planch using high-performance thin layer chromatography. Asian J Pharm Clin Res 2014;7:4.
24. Lavanya MS, Gnanamani A, Ilavarasan R. Physico-chemical, phytochemical and high-performance thin layer chromatography analysis of the whole plant of Orthosiphon thymiflorus (Roth.) sleesen. Asian J Pharm Clin Res 2015;8:1.
25. Geneva. ICH, Q2B, Guideline on Validation of Analytical Procedure: Methodology, International Conference on Harmonization; 1996. p. 1–8.
26. Geneva. ICH, Q2A, Text on Validation of Analytical Procedure, International Conference on Harmonization; 1994. p. 1–5.
27. Sarfaraj H, Sheeba F, Mohammad Ali, Sarfaraz Alam, Akhlakquer R, Srivastava AK. Phytochemical investigation and simultaneous estimation of bioactive lupeol and stigmasterol in Abutilon indicum by validated HPTLC method. J Coastal Life Med 2014;2 Suppl 5:394-401.
28. Rozylo JK, Janicka M. Different planar techniques for prediction of solute retention in column liquid chromatography. J Planar Chromatogr 1996;9 Suppl 6:418-24.
29. Weins C, Hauck HE. Advances and developments in thin layer chromatography. LC-GC Int 1996;14 Suppl 6:455-71.
30. Poole CF, Poole SK. Instrumental thin-layer chromatography. Anal Chem 1994;66 Suppl 1:27A-37A.
31. Meenu S, Sharma RG. Identifiaction, purification and quantification of andrographolide from Andrographis paniculata (burm. F.) nees by HPTLC at different stages of life cycle of the crop. J Curr Chem Pharm Sci 1994;3 Suppl 1:23-32.
32. Padma Sarojini D, Manjunatha P, Venkata R. Preliminary phytochemical screening and anthelmintic activity of Andrographis echioides nees. J Pharma Res 2015;5 Suppl 9:4801-3.
33. Ramasubramania R. Pharmacognostical phytochemical and anti-ulcer activity of Andrographis echioides (Acanthaceae). J Pharmacogn Phytochem 2014;3 Suppl 3:39-49.
34. Suthar AC, Banavaliker MM, Biyani MK, Priyadarsini Indira K, Sudarsan V, Mohan H. A high-performance thin layer chromatography method for quantitative estimation of lupeol in Crataeva nurvala. Indian Drugs 2001;38 Suppl 9:474-8.
35. Badami S, Gupta MK, Ramaswamy S, Rai SR, Nanjaian M, Bendell DJ, et al. Determination of betulin in Grewia titiaefolia by HPTLC. J Separation Sci 2004;27:129-31.
36. Maria Carmen, S Tan, Glenn G, Oyong, Chien-Chang Shen, Consolacion Y Ragasa. Secondary metabolites from Andrographis paniculata (Burm. f.) nees. Pharm Lett 2016;8 Suppl 13:157-60.
Statistics
276 Views | 857 Downloads
Citatons
How to Cite
Gurupriya, S., L. Cathrine, and P. Pratheema. “HPTLC METHOD FOR THE DETERMINATION OF LUPEOL FROM ANDROGRAPHIS ECHIOIDES LEAVES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 5, May 2018, pp. 102-7, doi:10.22159/ijpps.2018v10i5.24748.
Section
Original Article(s)