• Selma Houchi Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Setif 1, Setif 19000, Algeria.
  • Rachid Mahdadi Laboratory of Applied Biochemistry, Department of Biology, Faculty of Sciences, University of Ferhat Abbas setif-1, 19000, Algeria
  • Zakia Messasma Laboratory of Electrochemistry, Molecular Engineering and Redox Catalysis, Department of Process Engineering, Faculty of technology, University of Ferhat Abbas setif, 19000, Algeria.
  • Farida Sahli Laboratory of Microbiology, University Hospital of Setif, 19000, Algeria.


Objective: toevaluate Metallo-betalactamase (MBL) inhibitory effect and antimicrobial activity against Acinetobacter baumannii of methanolic and aqueous extracts of Terminaliachebuladried fruits.

Methods: effect of EDTA (Ethylenediaminetetraacetic acid), methanolic and aqueous extracts of dried fruits of T. chebula on Metallo-betalactamase activity was studied in vitroby using cephaloridin asasubstrate. The degree of inhibition was determined byIC50and the antimicrobial activities of both extracts against Acinetobacter baumannii were qualitatively and quantitatively assessed by the presence or absence of inhibition zones, MIC and MBC values.

Results: EDTA, methanolic and aqueous extracts inhibit metallo-betalactamases activity withIC50(3.98 mM, 18.45 and 19.68µg/ml), respectively. Polyphenols in aqueousand methanolic extracts are 513.094±3.061 and 496±2.856 µg GAE/mg, respectively.

Conclusion: These results indicate that both extracts of T. chebula are rich in polyphenols and have a strong inhibitory effect on metallo-betalactamases in a competitive manner compared to EDTA. In addition, both extracts of T. chebula were also screened for their remarkable antibacterial and bactericidal activity against Acinetobacter baumannii confirmed by low minimum inhibitory and bactericidal concentration (MIC and MBC).


Keywords: Metallo-betalactamases, Acinetobacter baumannii, Terminaliachebula, Cephaloridin, EDTA.


Download data is not yet available.

Author Biography

Selma Houchi, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Setif 1, Setif 19000, Algeria.
Department of Biochemistry, Faculty of Nature and Life Sciences


1. Goel V, Hogade SA, Karadesai SG. Prevalence of extended-spectrum beta-lactamases, AmpC beta-lactamase, and metallo-beta-lactamase producing Pseudomonas aeruginosa and Acinetobacterbaumannii in an intensive care unit in a tertiary care hospital. J Sci Soc 2013;40:28-31.
2. Rudgers GW, Huang W, Palzkill. Binding properties of a peptide derived from b-lactamase inhibitory protein. Antimicrob Agents Chemother 2001;45:3279-86.
3. Zhu K, Lu J, Ye F, Jin L, Kong X, Liang Z, et al. Structure-based computational study of the hydrolysis of New Delhi metallo-b-lactmase-1. Biochem Biophys Res Commun 2013;431:2-7.
4. Drawz SM, Bonomo RA. Three Decades of β-Lactamase Inhibitors. Clin Microbiol Rev 2010;23:160-201.
5. Shuchita D, Kamat SD, Kamat DV. Effect of aqueous extract of terminalia chebulaon metallobetalactamase. Int J Pharm Pharm Sci 2010;2 Suppl 4:172-5.
6. Bush K, Jacoby GA. Updated functional Classification of β-Lactamases. Antimicrob Agents Chemother 2010;54:969-76.
7. Yang KW, Feng L, Yang SK, Aitha M, LaCuran AE, Oelschlaeger P, et al. New b-phospholactam as a carbapenem transition state analog: synthesis of a broad-spectrum inhibitor of metallo-b-lactamases. Bioorg Med Chem Lett 2013;23:5855-9.
8. N’guessan JD, Dinzedi MR, Guessennd N, Coulibaly A, Dosso M, Djaman AJ, et al. Antibacterial activity of the aqueous extract of Thonningiasanguinea against Extended-Spectrum-ß-Lactamases (ESBL) producing Escherichia coli and Klebsiellapneumoniaestrains. Trop J Pharm Res 2007;6:779-83.
9. Gao H, Huang YN, Xu PY, Kawabata J. Inhibitory effect on a-glucosidase by thefruits of Terminaliachebula Retz. Food Chem 2007;105:628-34.
10. Lee HS, Koo YC, Suh HJ, Kim KY, Lee KW. Preventive effects of chebulic acid isolated from Terminaliachebula on advanced glycationendproduct-induced endothelial cell dysfunction. J Ethnopharmacol 2010;131:567-74.
11. Li HB, Cheng KW, Wong CC, Fan KW, Chen F, Jiang Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem 2007;102:771-6.
12. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem1976;72:248-54.
13. Genç Y, Özkanca R, Bekdemir Y. Antimicrobial activity of some sulfonamide derivatives on clinical isolates of staphylococusaureus. Ann Clin Microbiol Antimicrob 2008;7:17-22.
14. Verporte R, Ijin A, Tsoui A, Vandourne H, Baerheim M. Medicinal plants of Suriname. I. Antimicrobial activity of some medicinal plants. J Ethanopharmacol 1982;5:221-6.
15. Ambler RP. The structure of betalactamases. Philos Trans R Soc Lond B 1980;289:321-31.
16. Chen J, Liu Y, Fang M, Chen H, Lao X, Gao X, et al. Combined Support-Vector-Machine-Based virtual screening and docking method for the discovery of IMP-1 Metallo-ß-Lactamase Inhibitors. AM J Biomed Res 2013;1:120-31.
17. Nordmann P, Poirel L. Emerging carbapenemases in Gram-negative aerobes. Clin Microbiol Infect 2002;8:321-33.
18. Guo Y, Wang J, Niu G, Shui W, Sun Y, Zhou H, et al. A structural view of the antibiotic degradation enzyme NDM-1 from a superbug. Protein Cell 2011;2:384-94.
19. Li T, Wang Q, Chen F, Li X, Luo S, Fang H, et al. Biochemical Characteristics of New Delhi Metallo-b-Lactamase-1 Show unexpected difference to other MBLs. PLoS ONE 2013;8:e61914.
20. Saleem A, Husheem M, Härkönen P, Pihlaja K. Inhibition of cancer cell growth by crude extract and the phenolics of Terminaliachebularetz fruit. J Ethnopharmacol 2002;81:327-36.
21. Malekzadeh F, Ehsanifar H, Shahamat M, Levin M, Colwell RR. Antibacterial activity of black myrobalan (Terminaliachebula Retz) against Helicobacter pylori. Int J Antimicrob Agents 2001;18:85–8.
392 Views | 601 Downloads
How to Cite
Houchi, S., R. Mahdadi, Z. Messasma, and F. Sahli. “THE INHIBITORY EFFECT OF METHANOLIC AND AQUEOUS EXTRACTS OF TERMINALIA CHEBULA DRIED FRUITS ON METALLOBETALACTAMASES OF ACINETOBACTER BAUMANNII”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 579-82,
Original Article(s)