• Selma Houchi Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Setif 1, Setif 19000, Algeria.
  • Rachid Mahdadi Laboratory of Applied Biochemistry, Department of Biology, Faculty of Sciences, University of Ferhat Abbas setif-1, 19000, Algeria
  • Zakia Messasma Laboratory of Electrochemistry, Molecular Engineering and Redox Catalysis, Department of Process Engineering, Faculty of technology, University of Ferhat Abbas setif, 19000, Algeria.
  • Farida Sahli Laboratory of Microbiology, University Hospital of Setif, 19000, Algeria.


Objective: toevaluate Metallo-betalactamase (MBL) inhibitory effect and antimicrobial activity against Acinetobacter baumannii of methanolic and aqueous extracts of Terminaliachebuladried fruits.

Methods: effect of EDTA (Ethylenediaminetetraacetic acid), methanolic and aqueous extracts of dried fruits of T. chebula on Metallo-betalactamase activity was studied in vitroby using cephaloridin asasubstrate. The degree of inhibition was determined byIC50and the antimicrobial activities of both extracts against Acinetobacter baumannii were qualitatively and quantitatively assessed by the presence or absence of inhibition zones, MIC and MBC values.

Results: EDTA, methanolic and aqueous extracts inhibit metallo-betalactamases activity withIC50(3.98 mM, 18.45 and 19.68µg/ml), respectively. Polyphenols in aqueousand methanolic extracts are 513.094±3.061 and 496±2.856 µg GAE/mg, respectively.

Conclusion: These results indicate that both extracts of T. chebula are rich in polyphenols and have a strong inhibitory effect on metallo-betalactamases in a competitive manner compared to EDTA. In addition, both extracts of T. chebula were also screened for their remarkable antibacterial and bactericidal activity against Acinetobacter baumannii confirmed by low minimum inhibitory and bactericidal concentration (MIC and MBC).


Keywords: Metallo-betalactamases, Acinetobacter baumannii, Terminaliachebula, Cephaloridin, EDTA.


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Author Biography

Selma Houchi, Laboratory of Applied Biochemistry, Department of Biochemistry, Faculty of Nature and Life Sciences, University Setif 1, Setif 19000, Algeria.
Department of Biochemistry, Faculty of Nature and Life Sciences


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How to Cite
Houchi, S., R. Mahdadi, Z. Messasma, and F. Sahli. “THE INHIBITORY EFFECT OF METHANOLIC AND AQUEOUS EXTRACTS OF TERMINALIA CHEBULA DRIED FRUITS ON METALLOBETALACTAMASES OF ACINETOBACTER BAUMANNII”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 579-82,
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