CYTOTOXICITY OF METABOLITES PRODUCED BY ENDOPHYTIC FUNGUS CLADOSPORIUM SP. ISOLATED FROM MARINE MACROALGAE ON IN­VITRO MCF­7, HELA, AND DU-145 CELL LINES

  • Asri Peni Wulandari Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University
  • R. R. Indry Noviarin Examinati Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University
  • Madihah . Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University
  • Desi Harneti Putri Huspa Department of Chemistry, Faculty of Mathematics and Natural Sciences Padjadjaran University
  • Poniah Andayaningsih Department of Biology, Faculty of Mathematics and Natural Sciences, Padjadjaran University
  • Poniah Andayaningsih

Abstract

Objective: To investigate the in vitro cytotoxicity effect of the crude ethyl acetate extract of Cladosporium sp. on MCF-7, HeLa, and DU-145 cell lines.

Methods: In vitro cytotoxicity was evaluated by tetrazolium reduction assay. The percentage of cell inhibition was analyzed using probit analysis to obtain 50% inhibitory concentration (IC50). Morphological alteration of the cell lines after exposure with extract was observed under an inverted microscope.

Results: The ethyl acetate extract of the metabolite performed an anticancer activity for cancer cell line MCF-7, HeLa, and DU-145 with IC50 respectively 8.46 μg/ml; 9.87 μg/ml; and 98.03 μg/ml. The extract shows greater the anticancer activity and has strong antiproliferative on MCF-7 and HeLa cell line than DU-145. Confirmation morphological were observed under the inverted microscope showed a morphological change in cancer cells when incubated with the extract.

Conclusion: From the performed assay, the crude extract of Cladosporium sp. exhibit cytotoxic activity against MCF-7, HeLA, and DU-145.

Keywords: Cytotoxicity, Cladosporium sp., Endophytic, Fungus, MTT assay

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Wulandari, A. P., R. R. I. N. Examinati, M. ., D. H. P. Huspa, P. Andayaningsih, and P. Andayaningsih. “CYTOTOXICITY OF METABOLITES PRODUCED BY ENDOPHYTIC FUNGUS CLADOSPORIUM SP. ISOLATED FROM MARINE MACROALGAE ON IN­VITRO MCF­7, HELA, AND DU-145 CELL LINES”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 8, Aug. 2018, pp. 72-76, doi:10.22159/ijpps.2018v10i8.25181.
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