QUANTIFICATION OF NIGELLA SATIVA OIL (NSO) FROM BIODEGRADABLE PLGA NANOPARTICLES USING FTIR SPECTROSCOPY

  • Abd Almonem Doolaanea International Islamic University Malaysia
  • Ahmad Fahmi Harun International Islamic University Malaysia
  • Farahidah Mohamed Kulliyyah of Pharmacy, International Islamic University Malaysia, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

Abstract

Objective: Quantification of medicinal plant, N. sativa oil, in biodegradable nanoparticles fabricated from PLGA and chitosan was impossible due to the difficulty in the oil extraction-partition method of which the nanoparticle did not dissolve in any solvent.

Methods: FTIR method was developed to quantify the loading efficiency of NSO from the intact nanoparticles without the need to solubilise the nanoparticles and extract the NSO thereafter. Beer-Lambert law was applied in the quantification following selection of a few wave number combinations.

Results: The method exhibited linearity in the range NSO/PLGA=5-150% with R2=0.9911, RSD=1.68%, LOD=0.89% and LOQ=2.68%. NSO-PLGA nanoparticles revealed complete encapsulation of NSO (loading efficiency=101.5±2.2%) while chitosan-NSO-PLGA nanoparticle showed lower loading efficiency (84.5±1.7%) due to presence of the hydrophilic polymer, i. e. chitosan. This method is fast and easy to apply and does not require sample processing. The method will help to accelerate and improve routine characterisation of NSO nanoparticles during development and optimisation stage.

 

Keywords: Nigella sativa, FTIR, Loading efficiency, PLGA, Chitosan, Nanoparticle.

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References

1. Paarakh PM, Nigella sativa Linn. A comprehensive review. Indian J Nat Prod Resour 2010;1(4):409-29.
2. Ramadan MF. Nutritional value, functional properties and nutraceutical applications of black cumin (Nigella sativa L.): an overview. Int J Food Sci Technol 2007;42(10):1208-18.
3. Kanter M. Protective effects of nigella sativa on the neuronal injury in frontal cortex and brain stem after chronic toluene exposure. Neurochemical Res 2008;33(11): 2241-9.
4. Azzubaidi MS, et al. Protective effect of treatment with black cumin oil on spatial cognitive functions of rats that suffered global cerebrovascular hypoperfusion. Acta Neurobiol Exp (Wars) 2012;72(2):154-65.
5. Mohamadin AM, et al. Protective effects of Nigella sativa oil on propoxur-induced toxicity and oxidative stress in rat brain regions. Pestic Biochem Physiol 2010;98(1):128-34.
6. Sahni JK, et al. Neurotherapeutic applications of nanoparticles in Alzheimer's disease. J Controlled Rel 2011;152(2):208-31.
7. Ferri CP, et al. Global prevalence of dementia: a Delphi consensus study. Lancet 2006;366(9503):2112-7.
8. ALHaj NA, et al. Characterization of Nigella sativa L. essential oil-loaded solid lipid nanoparticles. Am J Pharmacol Toxicol 2010;5(1):52.
9. Bala I, S Hariharan, M Kumar. PLGA nanoparticles in drug delivery: the state of the art. Crit Rev Ther Drug Carrier Syst 2004;21(5):387-422.
10. Rao SB, CP Sharma. Use of chitosan as a biomaterial: studies on its safety and hemostatic potential. J Biol Materials Res 1997;34(1):21-8.
11. Baldrick P. The safety of chitosan as a pharmaceutical excipient. Regul Toxicol Pharmacol 2010;56(3):290-9.
12. Nickavar B, et al. Chemical composition of the fixed and volatile oils of Nigella sativa L. from Iran Zeitschrift Fur Naturforschung C 2003;58(9/10):629-31.
13. Cheikh-Rouhou S, et al. Nigella sativa L Chemical composition and physicochemical characteristics of lipid fraction. Food Chem 2007;101(2):673-81.
14. Ghosheh OA, AA Houdi, PA Crooks. High performance liquid chromatographic analysis of the pharmacologically active quinones and related compounds in the oil of the black seed (Nigella sativa L.). J Pharm Biol Anal 1999;19(5):757-62.
15. Kalinkova GN. Infrared spectroscopy in pharmacy. Vibrational Spectroscopy 1999;19(2):307-20.
16. Bassbasi M, et al. FTIR-ATR determination of solid non fat (SNF) in raw milk using PLS and SVM chemometric methods. Food Chem 2014;146(0):250-4.
17. Che Man YB, W Ammawath, MES Mirghani. Determining α-tocopherol in refined bleached and deodorized palm olein by Fourier transform infrared spectroscopy. Food Chem 2005;90(1–2):323-7.
18. Vongsvivut J, et al. Quantitative determination of fatty acid compositions in micro-encapsulated fish-oil supplements using Fourier transform infrared (FTIR) spectroscopy. Food Chem 2012;135(2):603-9.
19. Bureau S, et al. Application of ATR-FTIR for a rapid and simultaneous determination of sugars and organic acids in apricot fruit. Food Chem 2009;115(3):1133-40.
20. Reig FB, JVG Adelantado, MCM Moya Moreno. FTIR quantitative analysis of calcium carbonate (calcite) and silica (quartz) mixtures using the constant ratio method. Application to geological samples. Talanta 2002;58(4):811-21.
21. Liu W, et al. An investigation on the physicochemical properties of chitosan/DNA polyelectrolyte complexes. Biomaterials 2005;26(15):2705-11.
22. Banerjee T, et al. Preparation, characterization and biodistribution of ultrafine chitosan nanoparticles. Int J Pharm 2002;243(1–2):93-105.
23. Da Silva-Junior AA. et al. Thermal behavior and stability of biodegradable spray-dried microparticles containing triamcinolone. Int J Pharm 2009;368(1–2):45-55.
24. Guillén MD, N Cabo. Characterization of edible oils and lard by fourier transform infrared spectroscopy. Relationships between composition and frequency of concrete bands in the fingerprint region. J Am Oil Chem Soc 1997;74(10):1281-6.
25. Kökdil G, H Yılmaz. Analysis of the fixed oils of the genus Nigella L. (Ranunculaceae) in Turkey. Biochem Systematics Ecology 2005;33(12):1203-9.
26. Mohamed BA. Some characteristics of nigella (Nigella sativa L.) seed cultivated in Egypt and its lipid profile. Food Chem 2003;83(1):63-8.
27. Man C, et al. Poly (lactic acid)/titanium dioxide composites: Preparation and performance under ultraviolet irradiation. Polymer Degradation Stability 2012;97(6):856-62.
28. De Villiers MM, DE Wurster, K Narsai. Stability of lactic acid and glycolic acid in aqueous systems subjected to acid hydrolysis and thermal decomposition. J Soc Cosmetic Chemists 1997;48(4):165-74.
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How to Cite
Doolaanea, A. A., A. F. Harun, and F. Mohamed. “QUANTIFICATION OF NIGELLA SATIVA OIL (NSO) FROM BIODEGRADABLE PLGA NANOPARTICLES USING FTIR SPECTROSCOPY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 10, 1, pp. 228-32, https://innovareacademics.in/journals/index.php/ijpps/article/view/2529.
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