DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR QUANTITATIVE ESTIMATION OF NIMBOLIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM

  • Nasiruddin Ahmad Farooqui Department of Pharmacy, IIMT College of Medical Sciences, Meerut, U. P
  • Akalanka Dey Annamalai University, Department of Pharmacy, Annamalai Nagar, Tamil Nadu, India
  • G. N. Singh Drugs Controller General (I)& Secretary-Cum-Scientific Director, Central Indian Pharmacopoeial Commission, Ghaziabad.
  • T. S. Easwari Department of Pharmacy, IIMT College of Medical Sciences, Meerut, U. P.

Abstract

Objective: The present study was aimed to a simple, new, rapid and highly sensitive Reverse Phase - High Performance Liquid Chromatographic (RP-HPLC) method has been developed and an assay was validated for the quantitative estimation of nimbolide in solid dosage form.

Methods: The chromatographic separation was achieved on an Agilent 1200 series HPLC system C18 (250 mm x 4.6 mm x 2.5 μ) column packing by using isocratic mobile phase consisting of acetonitrile: water (90:10 v/v), flow rate was adjusted to 1.0 ml/min at a fixed wave length of 207 nm.

Results: The nimbolide was eluted at 2.880 ± 0.05 min and established a dynamic range of linearity over the concentration range of 3.125-200 ppm/ml (r2 = 0.9997 ± 0.005). The lower limit of detection and quantification was 0.007 ppm/ml and 0.021 ppm/ml respectively. The method was validated as per ICH guidelines. The accuracy of the method was determined by the recovery studies and the mean recovery was obtained 98.27%. Moreover the method was shown good reproducibility and recovery with percent relative standard deviation less than 2%.

Conclusion: Rapid, efficient and sensitive RP-HPLC method was developed for the estimation of nimbolide from the perspective of reducing the cost of analysis and time and thus by saving laboratory resources.

 

Keywords: Nimbolide, RP- HPLC, ICH guidelines.

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How to Cite
Farooqui, N. A., A. Dey, G. N. Singh, and T. S. Easwari. “DEVELOPMENT AND VALIDATION OF HPLC METHOD FOR QUANTITATIVE ESTIMATION OF NIMBOLIDE IN BULK AND PHARMACEUTICAL DOSAGE FORM”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, 1, pp. 432-7, https://innovareacademics.in/journals/index.php/ijpps/article/view/2539.
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