ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF ALKALOID EXTRACT OF CUCUMIS TRIGONUS ROXB
Objective: To evaluate the antioxidant and antibacterial efficiency of alkaloids of root, leaf, and fruit of Cucumis trigonus.
Methods: The antioxidant and antibacterial properties of alkaloid extracts were assessed by ferric thiocynate (FTC), 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, hydrogen peroxide radical scavenging assay, superoxide radical scavenging activity and ferric reducing power were analyzed separately for its inhibition percentage at different concentration (20µg, 30µg, 40µg and 50µg/ml) and antibacterial activity by agar disc diffusion and minimum inhibitory concentration (MIC) against Staphylococcus aureus (MTCC Code-9886), Pseudomonas aeruginosa (MTCC Code-6458).
Results: The highest percentage of extraction yield was observed in fruit followed by leaf and root. The maximum content of alkaloid was found in fruit and root compared to leaf. The scavenging efficiencies of the extracts increased with the increasing concentrations. Appreciable levels of total antioxidant activity by FTC (2.112±0.011%), DPPH radical scavenging activity (1.912±0.001%), superoxide radical scavenging activity (0.955±0.021%) and hydrogen peroxide radical scavenging activity (0.914±0.087 %) were observed at 50µg/ml of fruit extract. However, ferric reducing power was more in the root (0.893±0.0870%) compared to fruit (0.791±0.023%) and leaf (0.520±0.00 %) at 50µg/ml. The alkaloid extracts of root and fruit exhibited more antibacterial activity against Staphylococcus aureus (16.51±0.05 and 19.68±0.03 mm) and Pseudomonas aeruginosa (12.2±0.03 and14.2±0.06) at 100µg/ml than leaf. The minimum inhibitory concentration (MIC) of the root, leaf, and fruit was in the range of 3.125µg/ml to 35µg/ml for the pathogenic bacteria.
Conclusion: Data from the present results revealed that the alkaloid extracts of fruit and root of C. trigonus show good antioxidant and antibacterial potential than leaf. Hence, may be explored for the formation of new antibacterial with antioxidant drugs.
2. Kiritikar KR, Basu BD. Indian medicinal plants. Vol. 1. International Publishers 2Edn. Dehradun; 1999.
3. Naveena BM, Mendiratta SK, Anjaneyulu AS. Tenderization of buffalo meat using plant proteases from Cucumis trigonus Roxb (Kachri) and Zingiber officinale roscoe (Ginger rhizome). Meat Sci 2004;68:363-9.
4. Naik VR, Agshikar NV, Abraham GJ. Diuretic activity of Cucumis trigonus ROXB. J Ethanopharmacol 1981;3:15-9.
5. Naik VR, Agshikar NV, Abraham GJ. Analgesic and anti-inflammatory activity in alcoholic extracts of Cucumis trigonus ROXB. A preliminary Communication. Pharmacology 1980;20:52-6.
6. Salahuddin MD, Jalalpure SS. Antidiabetic activity of aqueous fruits extract of Cucumis trigonus Roxb. In streptozotocin-induced diabetic rats. J Ethanopharmacol 2010;127:565-7.
7. Thippeswamy BS, Thakker SP, Tubachi S, Kalyani GA, Netra MK, Patil Ulubelen A. Identification of steroidal and triterpenic compounds of Cucumis trigonus. Planta Med 1976;30:144-5.
8. Patil K, Mohammed Imtiaz S, Singh A, Bagewadi V, Gazi S. Hepatoprotective activity of Cucumis trigonus roxb. fruit against CCl4 induced hepatic damage in rats. Iranian J Pharma Res 2011;10:295-9.
9. Mangan F, Moreira M, Barros Z, Fernandes C, Mateus R, Finger F, et al. Research and extension activities implemented by the Umass ethnic crop program in 2009. Veg Note Veg Farmers 2010;21:1-16.
10. Cooke T. Lamiaceae. In: The flora of the presidency of Bombay. Vol. 1. Taylor and Francis, London; 1906.
11. Ramawat KG, Merillon JM. Biotechnology regulator of gene expression in the vasculature. Circ Res 2000;85:753-66.
12. Raghunathan K. Pharmacopoeial standards for ayurvedic formulations. Central council for Research in Indian Medicine and Homeopathy, E-25, Defense colony, New Delhi; 1976.
13. Usha Shome, Joshi P, Sharma HP. Pharmacognostic studies on Atemisia Scoparia Waldst and kit. Proc Indian Acad Sci (Plant Sci) 1984;93:151-64.
14. Harborne JB. The terpenoids. In: Phytochemical methods. Vol. 3. Chapman and Hall, London; 1973.
15. Mistuda H, Yuasumoto K, Iwami. Antioxidation action of Indole compounds during the antioxidation of linoliec acid. Nihon Eiyo Shokuryo Gakkai-Shi 1996;19:210-4.
16. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;26:1199-200.
17. Matinez AC, Marcelo EL, Marco AO, Moacyr M. Different responses of superoxide dismutase in freezing resistant Solanum curtibolum and freezing sensitive Solanum tuberosum subjected to oxidative and water stress. Plant Sci 2001;160:505.
18. Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intercellula communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003?8.
19. Oyaizu M. Studies on product of browning reaction prepared from glucose amine. Japan J Nutr 1986;44:307-15.
20. Indian Pharmacopoeia. Government of India, Ministry of Health and Family Welfare, Published by the Controller of Publications, Delhi; 1996.
21. Baron JE, Finegold SM. Methods of testing antimicrobial effectiveness. In: Bailey scotts Diagonostia. Microbiology. Mosby C Missouri; 1990. p. 171-94.
22. Bousselessela H, Yahia M, Mahboubi A, Benbia S, Yahia Massinissa. Antioxydant and antibacterial activity of alkaloids and terpenes extracts from Euphorbia granulate. Int J Bioengineering Life Sci 2013;7:166-9.
23. Gopalkrishnan S, Kalaiarasi T. Comparative phytochemical screening of the fruits of Cucumis trigonus ROXB and Cucumis sativus Linn. World J Pharm Pharm Sci 2014;3:1455-6.
24. Maiza Benabdesselam F, Khentache S, Bougoffa K, Chibane M, Adach S, Chapeleur Y, et al. Antioxidant activities of alkaloid extracts of two algerian species of Fumaria: Fumaria capreolata and Fumaria bastardii. Rec Nat Prod 2007;1:28-35.
25. Gill NS, Gaurav Sharma, Rashmi Arora. Isolation and characterization of Cucumis trigonus ROXB. seeds for their therapeutic potential. Int J Univers Pharm Bio Sci 2014;3:234-46.
26. Rosidah, Poppy Anjelisa Zaitun Hasibuan, Ginda Haro, Puteri Masri, Denny Satria. Antioxidant activity of alkaloid fractions of Zanthoxylum acanthopodium DC. fruits with 1,1-Diphenyl-2-picrylhydrazyl assay. Asian J Pharma Clin Res 2018;11:33-4.
27. Yamagishi SI, Edelstein D, Du XL, Brownlee M. Hyperglycaemic potentials collagen-induced platlet activation through mitochondrial superoxide overproduction. Diabetes 2001;50:1491.
28. Halliwell B, Gutteridge JMC. Free radicals in biology. Vol. 87. University Press; 1985.
29. Meir S, Kanner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compound in oxidative defense systems of various senescing leaves. J Agric Food Chem 1995;43:1813-7.
30. Gulcin I, Oktay M, Kufrevioglu OI, Aslan A. Determination of antioxidant activity of lichen Cetraria islandica L. Ach J Ethnopharmacol 2002;79:325-9.
31. Perry JJ, Staley JT. Microbiology. Sounders college publishing, New York: U. S. A; 1997.