• L. Krishnasamy Department of Biotechnology, Research scholar, Sathyabama University, Sholinganallur 600119, Chennai
  • P. Ponmurugan Department of Biotechnology, K. S. R. College of Technology,Tiruchengode
  • K. Jayanthi Department of Biotechnology, Hindustan college of arts and science, Kelambakkam, Chennai
  • V. Magesh School of life & environmantal science, Deakin University, Australia.


I aspalathoids, Hep3B cell line, AgNPs, Cell viability, Cancer


Objective: To study the cytotoxic & apoptotic efficacy of synthesized silver nanoparticles from I. aspalathoids against Hep3B cell line.

Methods: Hep3B cell line was purchased from American type culture collection (NCCS, Pune). The cells were treated with synthesized silver nanoparticles from I. aspalathoids for 24 & 48 hrs. Cell Viability was evaluated by MTT assay and DNA fragmentation assay.

Results: The IC50 (half maximal inhibitory concentration) value of the AgNPs on Hep3B cells was calculated to be194.65ng/ml. The treated cells were compared to the control group with respect to morphology. The mechanism of cell death was studied through ethidium bromide/acridine orange. The live untreated cells had well organized chromatin structures, whereas the treated cells had fragmented or condensed chromatin consisting of apoptotic nuclei. Therefore, the nuclear staining experiment shows that apoptosis started in 48h after addition of AgNPs to the culture medium.

Conclusion: The study demonstrated the possible of the use of biologically synthesized silver nanoparticles from I. aspalathoids as an effective drug against cancer.


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Leela A, Vivekandan M. Tapping the unexploited plant resources for the synthesis of silver nanoparticles. African J of Biotechnology 2008;7(17):3162-5.

Jain D, Kumar D,Kachhwaha S K, Kothari S L. Synthesis of plant-mediated silver nanoparticles using papaya fruit extract and evaluation of their anti microbial activities. J Nanomaterials and Biostructures 2009;4 (3):557-56.

Vdayasoorian C, Kumar V, Jayabalahrishnan RM. Phytofabrication, of silver nanoparticles using pomegranate. J of Nanomaterials and Biostructures 2011;2 (2):17-21.

Gurunathan S, Kalishwaralal K, Vaidyanathan R, Venkataraman D, Pandian SRK, Muniyandi J, et al. Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli Colloids and Surfaces B. J Biointerfaces 2009;1(1):328-35.

Kowshik M, Ashtaputre Kharazzi S, Vogel W Urban J, Kulkarni S, K Panikar. Extracellular synthesis of silver tolrent strain MKY3 K. M. J Nanotechnology 2003;14(1):95-100.

Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M, Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. J Biotechnology Progress 2006;22(2):577–83.

Gardea-Torresdey JL, Gomez E, Peralta-Videa JR, Parsons JG, Troiani H, Jose-Yacaman M. Alfalfa sprouts a natural source for the synthesis of silver nanoparticles Langmuir 2003;19 (4);1357-61.

Kanchana A, Balakrishna M, Anticancer effect of saponins isolated from solanum trilobatum leaf extract and induction of apoptosis in human larynx cancer cell line J of Pharmacy & Pharm Sci 2001;3(4):360-4.

Unno Y, Shino Y, Kondo F, Igarashi N, Wang G, Shimura R, et al. Oncolytic viral therapy for cervical and ovarian cancer cells by Sindbis virus AR339 strain. Clinical cancer research 2005;11(12):4553-60.

Abraham SA, Mc Kenzie C, Masin D, Ng R Harasym TO, Mayer LD, Bally MB. In vitro and in vivo characterization of doxorubicin and vincristine coencapsulated within liposomes through use of transition metal ion complexation and pH gradient loading. J Clinical Cancer Res 2004;10(2):728-38.

Byrd JC, Lucas DM, Mone AP, Kitner JB, Drabick JJ, Grever MR, A novel therapeutic agent with in vitro activity against human B-cell chronic lymphocytic leukemia cells mediates cytotoxicity via the intrinsic pathway of apoptosis. J Blood 2003;101(11):4547-50.

Safadi FF Xu J, Smock SL, Kanaan RA, Selim AH, Odgren PR Marks SC Jr, Owen TA. J Expression of connective tissue growth factor in bone:its role in osteoblast proliferation and differentiation in vitro and bone formation in-vivo. J Cell Physiol 2003;196(1):51-62.

RibbleD, Goldstein B, Norris DA, ShellmanYG, Apoptotic activity of caged xanthones from. J Garcinia BMC Biotechnology 2005;5(18):8-12.

Mohan S, Abdul AB, Abdelwahab SI, Al-Zubairi AS, Sukari MA, Abdullah R, et al. Typhonium flagelliforme induces apoptosis in CEMss cells via activation of caspase-9, PARP cleavage and cytochrome c release:its activation coupled with G0/G1 phase cell cycle arrest. J of Ethnopharmacology 2010;131(3):590-600.

Gurunathan S, Lee KJ, Kalimuthu K, Sheikpranbabu S, Vaidyanathan R, Eom SH, Antiangiogenic properties of silver nanoparticles. J Biomaterials 2009;30(31):6341-50.

Moaddab S, Hammed Ahari, Delavar Shahbazzadeh, Abbas Ali Motallebi, Amir Ali Anvar, Jaffar Rahman-Nya, et al. Toxicity Study of Nanosilver on Osteoblast Cancer Cell Line. J Int Nano Lett 2011;1(1):11-6.

Maribel G, Guzman Jean Dille, Stephan Godet. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity World Academy of Science. J Engineering and Technology 2008;43(2):357-64.

Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ. In vitro toxicity of nanoparticles in BRL 3A rat liver cells. J Toxicol In Vitr 2005;7(7):975-83.

Brown DM, Donaldson K, Borm PJ, Schins RP, Dehnhardt M, Gilmour P, et al. Calcium and ROS-mediated activation of transcription factors and TNF-alpha cytokine gene expression in macrophages exposed to ultrafine particles. Physiol Lung Cell Mol Physiol 2004;2(2):344-53.

Kim S, Cho JE, Choi J, Chung K, Park K Yi J, Ryu. Oxidative stress dependent toxicity of silver naaoparticles in human hepatoma cell. J Toxicol In Vitro 2009;23 (6):1076-84.

Cooke MC, Evans MD, Dizdaroglu M, Lunec J. Oxidative DNA damage:mechanisms, mutation, and disease. FASEB J 2003;17(10):1195-214.



How to Cite

Krishnasamy, L., P. Ponmurugan, K. Jayanthi, and V. Magesh. “CYTOTOXIC, APOPTOTIC EFFICACY OF SILVER NANOPARTICLES SYNTHESIZED FROM INDIGOFERA ASPALATHOIDS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, no. 8, Aug. 2014, pp. 245-8,



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