PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL EFFICACY STUDIES OF ANNONA SQUAMOSA FRUIT


Kowsalya V, Pruthivi Raj Behera, Elis Josna Mary, Anusha N, Vajrai R, Brindha P

Abstract


Objective: The present study was designed to evaluate the antimicrobial activity of lyophilized powder of Annona squamosa fruit. Phytochemical screening was carried out to detect the presence of major phytoconstituents.

Methods: Annona squamosa fruit pulps were lyophilized and made into powder, which was diluted with 0.1% DMF. This extract was tested against gram positive and gram negative bacteria employing Agar well diffusion method.

Results: The results showed that fruit extract possess antimicrobial activity against microbial isolates. The antibacterial activity increased with increase in mass of fruit extract, which was evidenced through zone of inhibition. The fruit extract showed significant activity when compared with standard positive antibiotic viz. Chloramphenicol (10 µg /disc). The preliminary phytochemical screening tests confirmed the presence of compounds such as alkaloids, terpenoids, flavonoids, steroids, saponins and glycosides as major constituents.

Conclusion: Based on the results it is concluded that Annona squamosa has good antimicrobial activity against human pathogens and is also rich in phytochemical constituents.


Keywords


Annona squamosa, Antibacterial, Phytochemical screening, Lyophilized fruit powder

| PDF | HTML |

References


Pomer KW, Lowe J, Crabtree S, Keller. Identification of Annonaceous acetogenins in the ripe fruit of the North American pawpaw (Asimina triloba). J Agric Food Chem 2009;57:8339-43.

Chenyoung, XU Shasha, Chen Jianwei. Antitumor activity of Annona squamosa seed extracts containing annonaceous acetogenins compounds. J Ethanopharmacol 2012;142(2):462-66.

BV Parthasaradhi, Madhurima Reddy, Mubarak Ali, Leela kumari. Antitumor activity of Annona squamosa seed extract is through the generation of free radicals and induction of apoptosis. Indian J Biochem Biophys 2004;41:167-72.

Vinut S Nandagaon, AR Kulkarni. In vitro Antioxidant and Cytotoxicity Activity of Aqueous and Alcoholic Extracts of Annona Squamosa. Res J Pharm Biol Chem 2012;3(4):479.

Gupta RK, Kesari AN, Murthy PS, Chandra R, Tandon V, Watal G. Hypoglycemic and antidiabetic effect of ethanol extract of leaves of Annona squamosa L. in experimental animals. J Ethanopharmacol 2005;99:75-81.

Ragavendra, Saiket Sen, Siva Rami Reddy. Synergistic activity of Tribulus terrestris and Annona squamosa extracts against alloxan induced diabetes & Hyperlipidemia in rats. J Pharma Science Monitor 2011;2:0976-7908.

Ragasa consolacion, Soriano geneveve, Tosses Oscar, Don-Ming-Jaw. Acetogenin from Annona muricata. J of Phar 2012;4(32).

Javed Aamir, Annu Kumari, Nadeem Khan, Siva Kumar Medam. Evaluation of the Combinational Antimicrobial Effect of Annona Squamosa and Phoenix Dactylifera Seeds Methanolic Extract on Standard Microbial Strains. Int Res J Biological Sci 2013;2(5):68-73.

Solomon-Wisdom G. O, Mohammed. Phytochemical Screening and Antimicrobial activities of Annona muricata (L) leaf extract. Am J Biol Chem Pharm Sci 2014;2(7).

Kamaruz zaman, Kalyani pathak. Pharmacognostical and Phytochemical Studies on the Leaf and Stem Bark of Annona reticulata. J Pharmacogn Phytochem 2012;1(5):2278-4136.

Anup Baranwal, Shikha Arora, Rakesh Kumar G, VR Prasad, Jeevena. Evaluation of the combinational antimicrobial effect of Prunus persia and Annona squamosa seeds methanolic extract on standard microbial strains. Global J Biosci Biotechnol 2013;2 (4):571-75.

Bansonde DS, Chavan MD. Studies of antimicrobial activity and photochemical analysis of citrus fruit juices against Selected Enteric Pathogens. Int Res J Pharm 2012;3 (11).

Udeozo IP, Akpaba Enem, Ugwu Okechukwu, Okoye NH, Umedum NL. Qualitative alkaloidal analyses of some selected Nigerian medicinal plants used in herbal treatment of diseases. Int J LifeSc Bt and Pharm Res, 2013;2:2250-3137.

Solomon Charles Ugochukwu1, Onuoha Ifeanyi. Preliminary phytochemical screening of different solvent extracts of stems bark and roots of Dennetia tripetala. Asian J Plant Sci Res.2013;3(3):10-13.




About this article

Title

PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL EFFICACY STUDIES OF ANNONA SQUAMOSA FRUIT

Keywords

Annona squamosa, Antibacterial, Phytochemical screening, Lyophilized fruit powder

Date

01-09-2014

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 6, Issue 8, 2014 Page: 286-288

Online ISSN

0975-1491

Statistics

871 Views | 343 Downloads

Authors & Affiliations

Kowsalya V
School of Chemical & Biotechnology, SASTRA University,
India

Pruthivi Raj Behera
School of Chemical & Biotechnology, SASTRA University,
India

Elis Josna Mary
School of Chemical & Biotechnology, SASTRA University,
India

Anusha N
School of Chemical & Biotechnology, SASTRA University,
India

Vajrai R
Centre for Advanced Research in Indian Systems of Medicine (CARISM), SASTRA
India

Brindha P
Centre for Advanced Research in Indian Systems of Medicine (CARISM), SASTRA
India


Article Tools



Refbacks

  • There are currently no refbacks.