IN VITRO ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF METHANOLIC EXTRACTS OF UVARIA CHAMAE LEAVES AND ROOTS

  • Nwokolo Lorreta Nwakaego Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu Campus, Enugu State Nigeria
  • Onyekwelu Kenechukwu Chibuike Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu Campus, Enugu State Nigeria
  • Ene Martin Chukwugekwu Department of Chemical Pathology, University of Nigeria Enugu Campus Medical Centre, Enugu State Nigeria
  • Adilieje Chioma Marylyn Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu Campus, Enugu State Nigeria
  • Ezechukwu Ifunanya Ngozi Department of Medical Biochemistry, College of Medicine, University of Nigeria, Enugu Campus, Enugu State Nigeria
  • Ezeh Richard Chukwunonye Department of Medical Biochemistry, ESUT College of Medicine, Parklane, Enugu Nigeria

Abstract

Objective: This study investigated the in vitro antioxidant and free radical potentials of methanol extracts of Uvaria chamae leaves and roots.


Methods: Fresh Uvaria chamae leaves and roots were air dried, pulverized and extracted using methanol. Phytochemical, total phenolic, flavonoids, antioxidant and tannin contents, DPPH, hydroxyl, and superoxide radical scavenging properties of the extracts were determined using standard methods.


Results: In vitro antioxidant potentials revealed that methanol extract of Uvaria chamae leaves contains vitamin A (4871±79.21 I. U) and vitamin C (1.72±0.02%) while the root extract contains vitamin A (673.28±0.00I. U) and vitamin C (1.66±0.01%). Both extracts had equal contents of vitamin E (8.83±0.04 mg/100g). The leaf extract scavenged 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) in a concentration dependent manner with the correlation coefficient (R2) of 0.839 and effective concentration (EC50) of 31.19 µg/ml, while the root extract scavenged DPPH with R2 of0.778 and EC50 of 14.00 µg/ml. The leaf and root extracts scavenged superoxide radical and hydroxyl radical with EC50 of 5.93 µg/ml and 719.45 µg/ml; 107.89 µg/ml and 912.01 µg/ml respectively compared to the EC50 of ascorbic standard (30.27 µg/ml) and EC50 of vitamin E standard (106.66µg/ml) respectively. The leaf extract showed significantly higher (p<0.05) anti radical power (ARP) of superoxide (0.17) compared to the root extract (0.0014) and the root extract showed significantly higher (p<0.05) ARP of DPPH (0.071) compared to the leaf extract (0.032).


Conclusion: The leaves and roots of Uvaria chamae are rich in natural antioxidants that can be exploited in the treatment of diseases related to oxidative stress.

Keywords: Uvaria chamae, DPPH, Superoxide radical, Hydroxyl radical

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Nwakaego, N. L., O. K. Chibuike, E. M. Chukwugekwu, A. C. Marylyn, E. I. Ngozi, and E. R. Chukwunonye. “IN VITRO ANTIOXIDANT AND FREE RADICAL SCAVENGING POTENTIAL OF METHANOLIC EXTRACTS OF UVARIA CHAMAE LEAVES AND ROOTS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 1, Jan. 2019, pp. 67-71, doi:10.22159/ijpps.2019v11i1.29330.
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