• Mumtaz Khan Mohammad Guest Lecturer, Department of Biochemistry, Bharathi Women's College, Broadway, Chennai
  • Monika Padmanabhan Department of Biochemistry, Bharathi Women's College, Broadway, Chennai



BRBG, HFD, BMI, Adiponectin, HMG CoA reductase, Blood lipids, Hyperlipidemia


Objective: Diet-inducedhyperlipidemia and obesity are the major risk factors for type II diabetes mellitus, hypertension, musculoskeletal and cardiovascular disorders (CVD). The objective of the present study is to furnish scientific proof for the lipid-lowering effect of β-glucan, a lead compound present in barley with a defined mechanism of action.

Methods: Obesity was induced in male albino Wistar rats by feeding ahigh-fat diet (HFD) for 14 w and were randomly divided into four groups of equal number (n=6). Group 1 and 2 served as control fed with normal diet (5% fat). Group 3 and 4 were fed HFD (23%fat) for 14 w. In addition, group 2 and 4 rats were administered orally with 200 mg/kg body weight of barley β glucan (BRBG) from the third week. After 14 w, the rats were sacrificed, and serum/plasma levels of total cholesterol (TC), phospholipids, triglycerides (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL) and adiponectin were determined.

Results: Biochemical changes were observed in weight gain, body mass index (BMI),adiposity index (ADI), total fat pad mass (TFP), blood lipids, LDL, lipid peroxides (LPO) and antioxidant enzyme activity of HFD fed rats when compared with BRBG co-administered rats. In addition, serum adiponectin levels and 3-hydroxy-3methyl-glutaryl-coenzyme Areductase(HMG CoA reductase)activity were elevated in rats administered BRBG along with HFD. Histological examination in HFD induced rats revealed a profound change in cell size with increased hypertrophy in visceral adipose tissue.

Conclusions: The results indicate that barley consumption could reverse most of these biochemical and histological changes in HFD fed rats owing to its hypolipidemic and antioxidant effect.


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Author Biographies

Mumtaz Khan Mohammad, Guest Lecturer, Department of Biochemistry, Bharathi Women's College, Broadway, Chennai

Department of biochemistry

Bharathi Women's College 


Monika Padmanabhan, Department of Biochemistry, Bharathi Women's College, Broadway, Chennai


Department of biochemistry

Bharathi Women's College 



Anitha M, Monisha DM, Chellam KJ. Prevalence of obesity and overweight among medicos in both male and female students. Asian J Pharm Clin Res 2016;9:289-91.

Chakkarwar VA, Kawtikwar P. Effect of peroxisome proliferator-activated receptor-alpha-agonists on diabetes induced acute kidney injury: role of oxidative stress and hyperlipidemia. Asian J Pharm Clin Res 2019;12:143-9.

Felber JP, Golay A. Pathways from obesity to diabetes. Int J Obes 2002;26 Suppl 2:39-45.

Kale M, Bindu S, Khadkikar P. Role of antioxidants and nutrition in oxidative stress: a review. Int J Appl Pharm 2015;7 Suppl 1:1-4.

Teresa AH, Noriyuki O, Rei S, Kenneth W. Adiponectin actions in the cardiovascular system. J Cardiores 2007;74:11-8.

Cooke D, Bloom S. The obesity pipeline: current stratergies in the development of anti-obesity drugs. Nat Rev Drug Discovery 2006;5 Suppl 11:919-31.

Lucia M, Sara M, Gabriella D, Antonio N, Eleonora P, Gabriella DR, et al. Oxidative stress in obesity: a critical compound in human diseases. Int J Mol Sci 2015;16:378-400.

Smith KM, Queenan KM, Thomas W, Fulcher RG, Slavin JL. Physiological effects of concentrated barley β-glucan in mildly hypercholesterolemic adults. J Am Coll Nutr 2008;27 Suppl 3:434-40.

Talati R, Baker WL, Pabilonia MS, White CM, Coleman CI. The effects of barley-derived soluble fiber on serum lipids. Ann Fam Med 2009;7 Suppl 2:157-63.

Ostman E, Rossi E, Larsson H, Brighenti F, Bjorck I. Glucose and insulin responses in healthy men to barley bread with different levels of (1→3;1→4)-β-glucans: predictions using fluidity measurements of in vitro enzyme digests. J Cereal Sci 2006;43 Suppl 2:230-5.

Giriwono PE. Extract of fermented barley attenuates chronic alcohol induced liver damage by increasing antioxidative activities. Int Food Res 2010;43 Suppl 1:118-24.

Rabey HA, Seeni MN, Amer HM. Efficiency of barley bran and oat bran in ameliorating blood lipid profile and the adverse histological changes in hypercholesterolemic male rats. BioMed Res Int 2013;4:1-10.

Nascimento AF, Sugizaki MM, Leopoldo AS, Lima-Leopoldo AP, Luvizotto RA, Nogueria CR. A hypercaloric pellet-diet cycle induces obesity and co-morbidities in wistar rats. Araq Bras Endocrinol Metab 2008;52:968-74.

Kyoko Kofuji, Ayumi Aoki, Kazufumi Tsubaki, Masanori Konishi, Takashi Isobe, Yoshifumi Murata. Antioxidant activity of β-glucan. ISRN Pharm 2011;2012:1-5.

Phillip B, Shapiro DJ. Improved methods for the assay and activation of 3-hydroxy-3-methyl glutaryl coenzyme a reductase. J Lipid Res 1970;20:588.

Zak B, Dickenman RC, White EG, Burnett U, Cherney PJ. Rapid estimation of free and total cholesterol. Am J Clin Pathol 1954;24:1307-15.

Van Handel E, Zilversmit DB. Micromethod for the direct determination of serum triglyceride. J Lab Clin Med 1957;50:152-7.

Bairaktari ET, Seferiadis KI, Elisaf MS. Evaluation of methods for the measurement of low-density lipoprotein cholesterol. J Cardiovasc Pharmacol Ther 2005;10:45-54.

Sinha AK. Calorimetric assay of catalase. Anal Biochem 1972;47 Suppl 2:389-94.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130-2.

Flohe L, Gunzler WA. Assay of glutathione peroxides. Methods Enzymol 1984;105:114-21.

Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxide. Methods Enzymol 1990;46:421-31.

Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochemic Biophys Acta 1979;582:67-78.

Bancroft JD, Gaamble M. Theory and practice of histological techniques 5th edition. Ch6: tissue processing and microtome including frozen, Ch8: the hematoxylins and eosin churchill livingston elsevier, Philadelphia, USA; 2018. p. 85-97, 125.

Monika P, Geetha A. Effect of hydroalcoholic fruit extract of Persea Americana Mill. On a high fat diet induced obesity: a dose response study in rats. Indian J Exp Biol 2016;54:370-8.

Aly NH. Effect of dietary oat and wheat bran on biochemical changes in rats fed high fat-high cholesterol diets. J Appl Sci Res 2012;8:598-604.

Ebrahim Mamaeghani M, Mohammadi S, Avefhosseini SR, Fallah P, Bazi Z. Adiponectin as a potential biomarker of vascular disease. Vasc Health Risk Manag 2015;11:55-70.

Amirkhizi F, Siassi F, Minaie S, Djalali M, Rahimi A, Chamari M. Is obesity associated with increased plasma lipid peroxidation and oxidative stress in women? ARYA Atheroscler 2007;2 Suppl 4:189-92.

Khalaf G, Abdel GM. Effect of barley (Hordeum vulgare) on the liver of diabetic rats: histological and biochemical study. Egypt J Histol 2008;31:245-55.



How to Cite

Mohammad, M. K., and M. Padmanabhan. “THE MODULATING EFFECT OF BARLEY β-GLUCAN EXTRACT ON HIGH FAT DIET INDUCED OBESITY-A BIOCHEMICAL STUDY IN ALBINO WISTAR RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 11, no. 4, Apr. 2019, pp. 49-54, doi:10.22159/ijpps.2019v11i4.31174.



Original Article(s)