IMPACT OF SOLVENT TYPES ON ANTIMICROBIAL ACTIVITIES OF ROOT EXTRACT OF CAESARIA TOMENTOSA

  • GAURI PAI ANGLE Department of Pharmacognosy P. E. S’s Rajaram and Tarabai Bandekar College of Pharmacy, Ponda-Goa,
  • YOGITA SARDESSAI Goa College of Pharmacy, Panjim–Goa

Abstract

Objective: The study deals with the antimicrobial activity of five solvent extracts of roots of Caesaria tomentosa against seven microorganisms: two gram-positive (Staphylococcus aureus and Bacillus subtilis), three gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimrium) and two fungal strains (Candida albicans and Aspergillus niger).


Methods: Roots of C. tomentosa were extracted with different solvents(n-hexane, ethanol, chloroform, acetone and water) and were subjected to antibacterial as well as antifungal screening by Well Diffusion Method. The Minimum Inhibitory Concentration(MIC) was also performed by two-fold dilution.


Results: The maximum inhibition zone at 50μg/ml concentration of n-hexane was 32 mm. These indicate that some active substances in C. tomentosa dissolved in varying degrees in the five solvents. The MIC for n-hexane was 1.6 μg/ml for S. aureus leading to a conclusion that the n-hexane extract was found to be the most potent.


Conclusion: All extracts were very effective against S. aureus. As for the solvents, the n-hexane extract had the best inhibitory effect among five solvents tested.

Keywords: Antimicrobial activity, Disc diffusion, Minimum Inhibitory Concentration

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Author Biography

GAURI PAI ANGLE, Department of Pharmacognosy P. E. S’s Rajaram and Tarabai Bandekar College of Pharmacy, Ponda-Goa,

Assistant Professor Department of Pharmacognosy P.E.S’s Rajaram and Tarabai Bandekar College of Pharmacy, Ponda-Goa

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How to Cite
ANGLE, G. P., and Y. SARDESSAI. “IMPACT OF SOLVENT TYPES ON ANTIMICROBIAL ACTIVITIES OF ROOT EXTRACT OF CAESARIA TOMENTOSA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 4, Feb. 2019, pp. 33-38, doi:10.22159/ijpps.2019v11i4.31398.
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