KINETICS AND MODELING OF TANNASE PRODUCTION USING ASPERGILLUS FOETIDUS IN BATCH FERMENTATION
Objective: To produce tannase enzyme using Aspergillus foetidus with red gram husk as substrate in batch fermentor and investigate the suitable un structured kinetic model for the system.
Methods: The present study was done via two steps. At first to study the maximum production of tannase enzyme by Aspergillus foetidus (MTCC 3557) using red gram husk as a substrate in a modular ferment or followed by to develop the kinetic model of tannase production.
Results: The maximum tannase activity and biomass concentration were found to be 157.26 U/ml and 7.12 g/l respectively at the end of 96 hours of fermentation. The biomass yield coefficient (YX/S) and the product yield coefficient (YP/S) were found to be 0.23 g of biomass/g of substrate and 21.2 U/g of substrate respectively. Logistic model, Luedeking-Piret model and substrate utilization kinetic model were found to represent closely the experimental data of growth kinetics, product formation kinetics and substrate utilization kinetics respectively.
Conclusion: Tannase enzyme production was studied using A. foetidus with redgram husk as substrate by modular fermenter and suitable models were predicted. The kinetic parameters were also estimated by fitting the data to the model using the Lineweaver-Burk method.
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