PROTECTIVE EFFECT OF ACACIA NILOTICA (BARK) AGAINST ANTI TUBERCULAR DRUG INDUCED HEPATIC DAMAGE AN EXPERIMENTAL STUDY
Objective: Protective Effect of Acacia nilotica (Bark) against anti tubercular drugs induced hepatic damage an experimental study.
Methods: Rats were divided into five different groups (n=6), the group I served as a control, Group II received Isoniazid-INH and rifampicin-RIF(50mg/kg) in sterile water, group III and IV served as treatment and received 200,400 mg/kg of 50% ethonolic extract of A. nilotica, and group V served as standard group and received silymarin (100mg/kg). All the treatments were given for 10-28 days and after rats were euthenised, blood and liver was collected for biochemical and histopathological studies, respectively.
Results: The 50% ethanolic bark extract of A. nilotica (200, 400 mg/kg p. o.) showed the remarkable hepatoprotective effect against Isoniazid-INH and rifampicin-RIF induced hepatic damage, and observed that it shows no any significant change in a normal posture, behavior and body weight in Wistar rats. The degree of protection was measured by biochemical and antioxidant parameters such as serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), total bilirubin, and the histopathological profile of liver also indicated the hepatoprotective nature of this drug.
Conclusion: The bark extracts of A. nilotica has showed dose dependent activity, among which at the dose level of 200 & 400 mg/kg. The further investigations, the bark extract of Acacia nilotica identify the active constituents responsible for hepatoprotection.
2. Wolf PL. Biochemical diagnosis of liver diseases. Indian J Clin Biochem 1999;14:59â€“90.
3. Pawinee P, Chai-ngam N, Chuncharunee A, Komaratat P, Suksamrarn A. Choleretic activity of phloracetophenone in rats, structureâ€“function studies using acetophenone analogues. Eur J Pharmacology 2000;387:221â€“7.
4. Ward FM, Daly MJ. Hepatic Disease In clinical pharmacy and therapeutics Churchill Livingstone: New York; 1999. p. 195-212.
5. Raghupati G, Chandra I, kailasama ASL, venkatesam P. Rifampicin induced release of hydrazine from INH. Am Rev Respir Dis 1986;133-1072.
6. Mohamed Saleem TS, Christina AJM, Chidambaranathan N, Ravi V, Gauthaman K. Hepatoprotective activity of Annona squamosa Linn. On experimental animal model. Int J Appl Res Nat Prod 2008;1(3):1-7.
7. Orhan DD, Orhan NU, Ergun E, Ergun F. Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats. J Ethnopharmacology 2007;112:145â€“51.
8. Khatri A, Arun G, Agrawal Shyam S. Evaluation of Hepatoprotective activity of aerial parts of Tephrosia purpurea L. and stem bark of Tecomella undulate. J Ethnopharmacology 2009;122:1-5.
9. Chaudhari NB. Hepatoprotective activity of cassia fistula seeds against paracetamol-induced hepatic injury in rats. Arch Pharm Sci Res 2009;218-21.
10. Lalit R, Bhatt J, Patel J. Hepatoprotective activity of ethanolic extracts of bark of Zanthoxylum armatum DC in CCl4 induced hepatic damage in rats. J Ethnopharmacology 2010;127:777â€“80.
11. Malviya S, Rawat S,Verma M, Kharaia A. Preliminary phytochemical investigations of Acacia nilotica linn plant. Current Pharm Res 2011;1(2):91-100.
12. Bansal VK, Goel RK. Gastroprotective effect of Acacia nilotica young seedless pod extract. Role of polyphenolic constituents. Asian Pacific J Tropical Med 2012;523-8.
13. Banso A. Phytochemical and antibacterial investigation of bark extracts of Acacia nilotica. J Medicinal Plants Res 2009;3(2):082-085.
14. Meena PD, Kaushik P, Shukla S, Soni AK, Kumar M, Kumar A. Anticancer and antimutagenic properties of Acacia nilotica (Linn.) on 7, 12-dimethylbenz (a) anthracene-induced skin papillomagenesis in Swiss albino mice. Asian Pac J Can Prev 2006;7:627-32.
15. Dhabhai K Batra A. Hormonal regulation impact on regeneration of acacia nilotica l. a nitrogen fixing tree. World Appl Sci J 2010;11(9):1148-53.
16. Selestin SD, Juliette K, Faustin D, Sylviane S, BarnabÃ© LNYN, Germain TS, et al. Acute and chronic anti-inflammatory effects of the aqueous extract of Acacia nilotica (L.) Del. (Fabaceae) pods. Acad J Med Plants 2013;1(1):001-5.
17. Sidra R, Usman AA, Sana R, Tariq J, Sheikh R. Antiviral activity of Acacia nilotica against Hepatitis C Virus in liver infected cells. Virology J 2011;8:220.
18. Kaur K, Michael H, Arora S, Harkonen P, Kumar S. In vitro bioactivity-guided fractionation and characterization of polyphenolic inhibitory fractions from Acacia nilotica (L.) Willd. ex Del. J Ethnopharmacol 2005;99:353-630.
19. Singh BN, Singh BR, Singh RL, Prakash D, Sarma BK, Singh HB. Antioxidant and anti-quorumsensing activities of green pod of A. nilotica L. Food Chem Toxicol 2009;47:778â€“86.
20. Tahir AE, Satti GMH, Khalid SA. Antiplasmodial activity of selected sudanese medicinal plants with emphasis on Acacia nilotica. Phytother Res 1999;13:474â€“8.
21. Solomon-Wisdom GO, Shittu GA. In vitro antimicrobial and phytochemical activities of Acacia nilotica leaf extract. J Med Plants Res 2010;4:1232-4.
22. Maity CR, Mandai B. Chemical and nutritional studies on the seed oil of Acacia arabica. J Am Oil Chem Soc1990;67:433-4.
23. Prakash L, Garg G. Chemical constituents of the roots of Millingtonia hortensis Linn. And Acacia nilotica (Linn.) Del. J Indian Chem Soc1981;58:96-7.
24. Rasool N, Tehseen H, Riaz M, Komal R, Muhammad Z, Mahmood Y, et al. Cytotoxicity studies and antioxidant potential of Acacia nilotica roots. Int J Chem Biochem Sci 2013;3:34-41.
25. Prasad V, Gupta SC. Inhibitory effect of bark and leaf decoctions on the activity of pectic enzymes of Alternaria tenuis. Indian J Exp Biol 1967;5:192-3.
26. Verma V, Singh BK. A review on Acacia nilotica linn. and its ethnobotony. phytochemical and pharmacological profile. IJPRD 2012:251-5.
27. Asad M, Aslam M, Munir TA, Nadeem A. Effect of acacia nilotica leaves extract on hyperglycaemia, lipid profile and platelet aggregation in streptozotocin induced diabetic rats. J Ayub Med Coll Abbottabad 2011;2:23.
28. Badar, Nadeem I, Khan Z, Nisar A, Shoaib M. In vitro and in vivo anthelmintic activity of acacia nilotica (l.) willd. ex delile bark and leaves. Pak Vet J 2011;31(3):185-91.
29. Ahmad M, Zaman F, Sharif T, Zabta M. Antidiabetic and hypolipidemic effects of aqueous methanolic extract of acacia nilotica pods in alloxan-induced diabetic rabbits scand. J Lab Anim Sci 2008;1:35.
30. Zimmerman M. Ethical guidelines for investigation of experimental pain in conscious. Animals Pain 1983;16:109-10.
31. Trease GE, Evans WC. Pharmacognosy. 13th ed. Bailliere Tindall Ltd, London; 1989. p. 176-80.
32. Harborne JB. Phytochemical method. 3rd ed. Chapman and Hall, London; 1993. p. 135-203.
33. Pal R, Vaiphei K, Sikander Arbab, Singh K, Rana Satya V. Effect of garlic on isoniazid and rifampicin-induced hepatic injury in rats. World J Gastroenterol 2006;12(4):636-9.
34. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamate oxaloacetate transaminase. Am J Clin Pathol 1957;28:53-6.
35. King J. The hydrolases-acid and alkaline phosphatases. In Practical clinical enzymology. London Nostrand Company Limited; 1965. p.191-208.
36. Malloy HT, Evelyn KA. The determination of bilirubin with the photometric colorimeter. J Biol Chem 1937;119:481-49.
37. Lillie RD. Histopathological techniques and practical histochemistry. Mc Grow Hill Book Company: New York; 1965.
38. Garner P, Holmes A, Ziganshina L. Tuberculosis. Clin Evid 2004;11:1081-93.
39. Sodhi CP, Rana SV, Mehta SK, Valphei K, Attari S, Mehta S. Study of oxidative stress in isoniazidrifampicin induced hepatic injury in rats. Drug Chem Toxicol 1997;20:255-69.
40. Thomas BS, Wong LT, Zeitz W, Solomonraj G. Isoniazid metabolism in the rabbit and the effect of rifampicin pretreatment. Res Commun Chem pathol Pharmacol 1981;33:235-47.
41. Santhosh S, Sini TK, Anandan R, Mathew PT. Effect of chitosan supplementation on antitubercular drugs-induced hepatotoxicity in rats. Toxicology 2006;219:53-9.
42. Man-Fung Y, Takanobu K, Masashi M, Annie On-On, John C, He-Jun CHY, et al. Clinical outcome and virologic profiles of severe hepatitis B exacerbation due to YMDD mutations. J Hepatol 2003;39:850-5.
43. Shishoo CJ, Shar SA, Rathod IS, Savale SS, Vora MJ. Impaired bioavailability of rifampicin in presence of isoniazid from fixed dose combination (FDC) formulation. Int J Pharm 2001;228:53-6.
44. Anon X. The resurgence of tuberculosis: a call for commitment. General policy topics. WHO Drug Info 1997;5:39-40.
45. Nkosi CZ, Opoku AR, Terblanche SE. Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCl4-induced liver injury in low protein fed rats. Phy Res 2005;19:341â€“5.
46. Shah M, et al. Evaluation of the effect of aqueous extract from powders of root, stem, leaves and whole plant of phyllanthus debilis against CCL4 induced rat liver dysfunction. Indian Drugs 2002;39:333-7.