PHYTOCHEMICAL SCREENING, FUNCTIONAL GROUPS AND ELEMENTAL ANALYSIS OF LEAF EXTRACT OF IPOMOEA OBSCURA (L) KER-GAWL
Objective: The aim of the study was to find out the phytochemical constituents, and to evaluate the functional group and elemental analysis of ethanol leaf extract of Ipomoea obscura (L.).
Methods: The secondary metabolites was analyzed for various extracts by using standard techniques. The metabolic activites can be measured by using FTIR and SEM-EDX.
Results: Phytochemical screening revealed the presence of alkaloids, glycosides, steroids, terpenoids, flavonoids, saponins and fatty acid and lipids in the extract. FTIR was used to identify various functional groups. Scanning Electron Microscope (SEM) with an energy dispersive X- ray spectrometer (EDX) showed the presence of calcium, magnesium, silicon, chloride, oxygen and potassium in ethanol leaf extract.
Conclusion: The results suggested that the ethanol leaf extracts of Ipomoea obscura (L.) showed the presence of most of the secondary metabolites, functional groups and trace elements.
2. Alston RE, Turner BL. Biochemical systematic: Prentice Hall New Jersey;1963.
3. Eckart E. Solanaceae and Convolvulaceae â€“ seconday metabolite 2008:1:637.
4. Christophe W, Pharm D. Ethnopharmacology of medicinal plants: Asia and the Pactic: Humana press;2002:1:69.
5. Shahina A. Handbook of Arabian medicinal plants: edd.-1 CRC publication; 1994. p. 90.
6. Srinivasan R, Chandrasekar MJN, Nanjan MJ, Suresh B. Free radical scavenging activity of Ipomoea obscura (L.) Ker-Gawl. J Nat Rem 2008:7(2):184-8.
7. Paech D, Tracey MV. Modern methods of plant analysis. 4thEd. Wieland: Springer Verlag;1955. p. 373-1.
8. Wolkers WF, Hoekstra AF. Aging of dry desiccation-tolerant pollen does not affect protein secondary structure. Plant Physiol 1995;109:907-15.
9. Wei ZL, Dong L, Tian ZH. Fourier transform infrared spectrometry study on early stage of cadmium stress in clover leaves. Pak J Bot 2009;41:1743-50.
10. Muruganatham S, Anbalagan G, Ramamurthy N. FT-IR and sem-eds comparative analysis of medicinal plants, Eclipta alba HASSK and Eclipta prostrata linn. Rom J Biophys 2009;19;285-94.
11. Shirwaikar A, Malini S, Kumari SC. Protective effect of Pongamia pinnata flowers against cisplatin and gentamicin iduced nephrotoxicity in rats. Indian J Exp Biol 2003;1:58-62.
12. Ambasta SP, Ramachandran K, Kashyapa K, Chand R. Useful plants of India. Publication and information directorate. Council of Scientific and Industrial Research: New Delhi;1986. p. 443-7.
13. Kokate CK, Purohit AP, Gokhale SB. Practical pharmacogonosy. 1st Ed. Vallabh prakashan: Delhi;1998.
14. Narayana KR, Reddy MS, Chaluvadi MR, Krishna DR. Bioflavonoids classification, pharmacology, biochemical effects and therapeutic potential. Ind J Pharmacol 2001;33:2-16.
15. Rao UP, Brahman M, Saxena HO. Phytochemical surrey of Marurbhanj, Ganjam and Puri Dist. (Orissa) for tannins, Saponins, Flavonoids. Ind Drug 1984;22:503-7.
16. Sharma SD, Chishti AM, Koul MK. Phytochemical survey of plants from Kashmir-II. Indian Drugs 1984;22:187-95.
17. Thenmozhi M, Bhavya PK, Rajeshwari S. Compound Identification Using HPLC and FTIR In Eclipt alba and Emilia sonchifolia. Int J Eng Sci Tech 2011;3:292-8.
18. Kalaiselvi M, Gomathi D, Vidya B, Uma C. Evaluation of Antioxidant potential and fourier transform infrared spectroscopy analysis of Ananus comosus (L.) Merr peel. Int Res J Pharm 2012;3:237-42.
19. Griffiths PR, De Haseth JA. Fourier transform infrared spectroscopy. John Wiley and Sons: New York;1986. p. 656.
20. Lin HX, Sun SQ, Lv GH, Chan KK. Study on Angelica and its different extracts by Fourier transform infrared spectroscopy and two-dimensional correlation IR spectroscopy. Mol Biomol Spec 2006;64:321-6.
21. Udayakumar R, Begum VH. Elemental analysis of medicinal plants used in controlling infectious diseases. Hamdard Med 2004;67:35-6.
22. Bibi S, Dastagir G, Hussain F, Sanaullah P. Elemental composition of Viola odorata Linn. Pak J Pl Sci 2006;12:141-3.
23. Bahadur A, Chaudhry Z, Jan G, Danish M, Rehman A, Ahmad R, et al. Nutritional and elemental analyses of some selected fodder species used in traditional medicine. Afr J Pharm Pharmacol 2011;5:1157-61.