ESTIMATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF CRUDE EXTRACTS OF THEVETIA PERUVIANA (PERS.) K. SCHUM.
Objective: The aim of this investigation was to estimate antioxidant and antibacterial activity of different crude extracts of Thevetia peruviana (Pers.) K. Schum.
Methods: Aqueous and alcoholic crude extracts of different plant parts were obtained and assessed for their antioxidant as well as antibacterial activity. These activities were determined by using standard protocols with some modifications. Aluminium chloride colorimetric method was used to estimate total flavonoid content and total phenolic content was measured by Folin-ciocalteu method.
Results: Free radical scavenging activity was recorded highest in fruits and lowest in root's extracts. The maximum phenolic and flavonoids content was found in fruits (TPC33.59Â±0.385mg GAE/ g DW, TFC6.98Â±0.22 mg QE/g DW). Minimum phenol content was found in root's extracts (21.47Â±0.160mg GAE/ g DW) while leaves possess lowest flavonoid content (4.69Â±0.17 mg QE/g DW). In case of antibacterial activity, aqueous and ethanol extracts of Thevetia peruviana flowers showed maximum antibacterial activity against Proteus vulgaris with maximum zone of inhibition with a value of 18.5Â±0.5 mm and 15.5Â±1.322 mm respectively. Ethanol extract of Thevetia peruviana fruits exhibited maximum antibacterial activity against Proteus vulgaris with a value of 13.5Â±0.5 mm for the zone of inhibition.
Conclusion: This investigation finds that methanol extracts of Thevetia peruviana has significant antioxidant activity. These crude extracts can further purify and can be used for development of anti-oxidative pharmaceutical compounds. Aqueous and ethanol extracts of Thevetia peruviana fruits and flowers have good antibacterial potential. But care should be taken as the plant has toxic properties also.
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