• Khaled Abo-EL-Sooud Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt
  • Mohamed Hashem Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt,
  • Yasmina ABD ELHAKIM Department of Forensic medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
  • Gehan kamel Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt,
  • Mona ELEIWA Department of Botany, Faculty of Science, Cairo University, Giza, Egypt,
  • Ali Gab-Alllah The Holding Company for Biological Products & Vaccines VACSERA


Objective: Daily exposure to food preservatives constitutes a major crisis to children especially during vaccination so; the aim of the study is to assess the effect of sodium nitrite on the immune responses against Rift Valley fever vaccine (RVFV) in Swiss mice.

Methods: Mice were divided into four equal groups: group 1 (control) was orally administrated with distilled water (2 ml/kg b. wt.); group 2 was orally administrated with the acceptable daily intake (ADI) of sodium nitrite 0.07 mg/kg b. wt. daily for 21 d, group 3 was vaccinated with inactivated RVFV two times and group 4 was orally given sodium nitrite and vaccinated as group 3. Blood samples were collected from all groups two weeks after booster vaccination. The leucocytic indices and the neutrophil-lymphocyte ratio (NLR) were determined to assess the cell-mediated immunity. The humoral immunity was evaluated using direct enzyme-linked immune-sorbent assay (ELISA) test and serum neutralization test (SNT).

Results: Sodium nitrite significantly increased the neutrophil index and the NLR whereas; it decreased the total leucocytic count and lymphocyte index in both non-vaccinated and vaccinated mice. Moreover, sodium nitrite significantly decreased both the IgG titer and the efficiency of vaccination through increasing the ED50 value.

Conclusion: Sodium nitrite existed an immune-suppressive effect on both cellular and humoral immune responses in mice.

Keywords: Sodium nitrite, Rift Valley fever, Immunity, Antibodies, Challenge


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How to Cite
Abo-EL-Sooud, K., M. Hashem, Y. ABD ELHAKIM, G. kamel, M. ELEIWA, and A. Gab-Alllah. “EFFECT OF SODIUM NITRITE EXPOSURE ON THE IMMUNE RESPONSES AGAINST OF RIFT VALLEY FEVER VACCINE IN MICE”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 11, no. 7, July 2019, pp. 28-31, doi:10.22159/ijpps.2019v11i7.33443.
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