Comparative evaluation of Total Phenolic Content, Total Flavonoid Content and DPPH free radical scavenging activity of Different Plant Parts of Vitex negundo L.COMPARATIVE EVALUATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT AND DPPH FREE RADICAL

  • Hemlata Singh university Of Rajasthan
  • Anupma Dixit University of Rajasthan
  • R. A. Sharma University of Rajasthan
  • Archna Sharma R. L. Saharia Govt College

Abstract

Objective: The study was aimed to comparatively evaluate Total Phenolic Content, Total Flavonoid content and DPPH free radical scavenging activity of the methanolic extracts of different plant parts (leaves, stems, flowers, fruits and roots) of Vitex negundo.

Methods: In this study, quantitative estimation of total phenolic and flavonoid contents were performed by using Folin–Ciocalteu method and aluminum chloride colorimetric method respectively. The free radical scavenging activities of the methanolic extracts of different plant parts were examined using established DPPH (2,2-Diphenyl-1-Picrylhydrazyl) assay.

Results: The highest Total Phenolic Content was found in flowers (71.21 ± 0.855 mg Gallic Acid Equivalent/ g dry weight of extract) while Total Flavonoid content was observed in leaves (27.32 ± 0.205 mg Quercetin Equivalent/g dry weight of extract). The highest free radical scavenging activity was recorded in flowers with IC50 value (82.25 ± 0.174 µg/ml) thus the most potent antioxidant part of the plant. Free radicall scavenging activity of the extracts was also found to increase in a dose dependent manner.

Conclusion: These findings suggest that methanolic extracts of different plant parts of Vitex negundo contain significant amount of phenols and flavonoids and also have significant antioxidant activity therefore it can be used in formulation of many antioxidant products and can be used as an important source of natural antioxidants.

 

Keywords: Vitex negundo, Total Phenolic content, Total Flavonoid content, DPPH Free radical scavenging activity, Antioxidant Activity

Downloads

Download data is not yet available.

References

1. Sannigrahi S, Mazumder UK, Pal D, Mishra SL. Hepatoprotective potential of methanol extract of Clerodendrum infortunatum Linn. Against CCl4 induced hepatotoxicity in rats. Indian J Exp Biol 2009;l5(20):394-9.
2. Willett WC. Balancing life-style and genomics research for disease prevention. Sci 2002;296:695-8.
3. Ito N, Fukushima S, Tsuda H. Carcinogenicity and modification of carcinogenic response by BHA, BHT and other antioxidants. Crit Rev Toxicol 1985;15:109-50.
4. Jayaprakasha GK, Jaganmohan Rao L. Phenolic constituents from lichen Parmotrema stuppeum (Nyl.) Hale and their antioxidant activity. Z Naturforsch 2000;55C:1018-22.
5. Cai YZ, Sun M. Antioxidant activity of Betalins from amaranthacea. J Agric Food Chem 2003;51:2288-94.
6. Khare CP. Indian medicinal plants: an illustrated dictionary. New York: Springer Science and Business Media; 2007. p. 710.
7. De Padua LS, Bunyapraphatsara N, Lemmens RHMJ. Medicinal and Poisonous Plants, Plant Resources of South East Asia. Backhuys Publishers Leiden; 1999.
8. Tiwari OP, Yamini BT. Antioxidant properties of different fractions of Vitex negundo. Linn Food Chem 2007;100:1170-6.
9. Nadkarni KM. Indian Materia Medica. Vol.1. Bombay Popular Prakashan; 2002. p. 1278-80.
10. Anonymous. The Wealth of India, Raw Materials. Vol.10. NewDelhi: Council of Scientific and Industrial Research; 2003. p. 158-60.
11. Singh P, Mishra G, Jha KK, Kumar V, Khosa RL. Chemical composition and antimicrobial activity of essential oil of leaves of Vitex negundo L. Int J Chem Tech Res 2010;2(3):1688-90.
12. Tandon VR. Medicinal uses and biological activities of Vitex negundo. Nat Prod Rad 2005;4(3):162-5.
13. Venkata SSN, Kantamreddi y, Nagendra L, Kasapu VVVS. Phytochemical analysis of some important Indian plant species. Int J Pharm Bio Sci 2010;1(4):351-7.
14. McDonald S, Prenzler PD, Autolovich M, Robards K. Phenolic content and antioxidant activity of olive extracts. Food Chem 2001;73:73-84.
15. Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Analy 2002;10:178-82.
16. Blois MS. Antioxidant determination by use of Stable free radicals. Nat 1958;29:1199-200.
17. Gulcin I. Antioxidant activity of L-Adrenaline: an activity structure insight. Chem Biol Interact 2009;179(2-3):71-80.
18. Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Fazelian M, Eslami B. In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Pharmacogn Mag 2009;4(18):123-7.
19. Shahidi F, Wanasundara PKJPD. Phenolic anti-oxidants. Crit Rev Food Sci Nutr 1992;32:67-103.
20. Sharma SK, Gupta VK. In vitro antioxidant studies of Ficus racemosus Linn. Pharmacogn Mag 2008;4:70-4.
21. Benavente-Garcia O, Castillo J, Marin FR, Ortuno A, Del-Rio JA. Uses and properties of citrus flavonoids. J Agric Food Chem 1997;45(12):4505-15.
22. Dehpour AA, Ebrahimzadeh MA, Nabavi SF, Nabavi SM. Antioxidant activity of methanol extract of Ferula assafoetida and its essential oil composition. Grasas Aceites 2009;60(4):405-12.
Statistics
717 Views | 1236 Downloads
How to Cite
Singh, H., A. Dixit, R. A. Sharma, and A. Sharma. “Comparative Evaluation of Total Phenolic Content, Total Flavonoid Content and DPPH Free Radical Scavenging Activity of Different Plant Parts of Vitex Negundo L.COMPARATIVE EVALUATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT AND DPPH FREE RADICAL”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 2, Dec. 2014, pp. 144-7, https://innovareacademics.in/journals/index.php/ijpps/article/view/3361.
Section
Original Article(s)