Comparative evaluation of Total Phenolic Content, Total Flavonoid Content and DPPH free radical scavenging activity of Different Plant Parts of Vitex negundo L.COMPARATIVE EVALUATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT AND DPPH FREE RADICAL

  • Hemlata Singh university Of Rajasthan
  • Anupma Dixit University of Rajasthan
  • R. A. Sharma University of Rajasthan
  • Archna Sharma R. L. Saharia Govt College


Objective: The study was aimed to comparatively evaluate Total Phenolic Content, Total Flavonoid content and DPPH free radical scavenging activity of the methanolic extracts of different plant parts (leaves, stems, flowers, fruits and roots) of Vitex negundo.

Methods: In this study, quantitative estimation of total phenolic and flavonoid contents were performed by using Folin–Ciocalteu method and aluminum chloride colorimetric method respectively. The free radical scavenging activities of the methanolic extracts of different plant parts were examined using established DPPH (2,2-Diphenyl-1-Picrylhydrazyl) assay.

Results: The highest Total Phenolic Content was found in flowers (71.21 ± 0.855 mg Gallic Acid Equivalent/ g dry weight of extract) while Total Flavonoid content was observed in leaves (27.32 ± 0.205 mg Quercetin Equivalent/g dry weight of extract). The highest free radical scavenging activity was recorded in flowers with IC50 value (82.25 ± 0.174 µg/ml) thus the most potent antioxidant part of the plant. Free radicall scavenging activity of the extracts was also found to increase in a dose dependent manner.

Conclusion: These findings suggest that methanolic extracts of different plant parts of Vitex negundo contain significant amount of phenols and flavonoids and also have significant antioxidant activity therefore it can be used in formulation of many antioxidant products and can be used as an important source of natural antioxidants.


Keywords: Vitex negundo, Total Phenolic content, Total Flavonoid content, DPPH Free radical scavenging activity, Antioxidant Activity


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Singh, H., A. Dixit, R. A. Sharma, and A. Sharma. “Comparative Evaluation of Total Phenolic Content, Total Flavonoid Content and DPPH Free Radical Scavenging Activity of Different Plant Parts of Vitex Negundo L.COMPARATIVE EVALUATION OF TOTAL PHENOLIC CONTENT, TOTAL FLAVONOID CONTENT AND DPPH FREE RADICAL”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 2, Dec. 2014, pp. 144-7,
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