THE GRAPHENE CONJUGATED USNIC ACID NANO FORMULATION FOR THE TREATMENT OF TOPICAL FUNGAL INFECTION
PREPARATION OF GUN and GUC
Objective: Lichens represent symbiotic commensalism between algae and fungi and a very rich source of different secondary metabolite including usnic acid. Usnic acid posses various pharmacological activities including antibacterial and antifungal activity. Since this secondary metabolite is poorly soluble in water that’s why its bioavailability is low. Hence to enhance the bioavailability of usnic acid, nano-conjugate was formulated with the help of graphene used as a carrier in the preparation of nano-conjugate. Graphene having also antibacterial activity, so the conjugate of graphene and usnic acid is developed to accomplished the higher anti-fungal activity. Method: Nano-precipitation method by sonication was adopted to formulate the conjugate. Results: SEM study shows that the conjugate is in the nano range and possess spherical shape. FTIR study shows no interaction between the drug and the carrier. The result of in-vitro drug release study shows that the conjugate posses higher drug release rate as compare to the drug alone. Topical drug administration is more suitable for the treatment of the fungal infection, so the nano-conjugate was incorporated into the ointment by geometric mixing. The viscosity and the spreadability test were performed on the different formulations of the ointment and the suitable one was selected for the topical administration. Conclusion: Anti-fungal study had been performed on the Wistar albino rats for 6 days. Skin culture of rats was performed for the formation of the fungal colonies. The anti-fungal study revealed that the nano-form of the conjugate possess higher anti-fungal activity than the normal formulation of usnic acid and graphene.
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