UPREGULATION OF trxB EXPRESSION BY PIPER BETEL LINN ETHANOLIC EXTRACT SUGGESTS INDUCTION OF OXIDATIVE STRESS WHICH POTENTIALLY CONTRIBUTES TO KILLING OF STAPHYLOCOCCUS AUREUS IN VITROUPREGULATION OF TRXB EXPRESSION BY PIPER BETEL LINN ETHANOLIC EXTRACT SU

  • Roslinah Mohamad Hussain Universiti Teknologi MARA
  • Farah Khalid Adam Ho Universiti Teknologi MARA
  • Maimunah Mustakim Universiti Teknologi MARA
  • Hamdan Suhaimi Universiti Teknologi MARA

Abstract

Objective: In this study, mRNA expressions of thioredoxin reductase B and catalase (encoded by trxB and katA respectively) in S. aureus were quantitated in response to P. betel ethanolic extract to determine its regulatory potential on oxidoreductases that may affect the organism’s ability to survive oxidative damage within the host.

Methods: P. betle ethanolic extract was prepared using the rotary evaporator and predetermined MIC (5mg/ml) for S. aureus (ATCC25923) was used in gene expression analyses that were performed using real-time PCR. Standardized bacterial inoculum (1 x108 cfu/ml) harvested at mid exponential phase were used in all assays. Total cytoplasmic protein concentrations were performed using the Bradford assay as per manufacturer’s instruction.

Results: trxB was expressed 3.95 fold higher in S. aureus cells treated with the extract (p<0.05) whereas expression of katA was only 0.0083 compared to untreated cells. A corresponding increase (87%) in total cytoplasmic protein was observed in cells treated with the extract.

Conclusion: Our observations suggest that P. betle ethanolic extract exerts oxidative stress on S. aureus causing upregulation of trxB. Further, it down regulates expression of katA (catalase) which may contribute to reduced ability of the organism to survive damage caused by phagocytic cells.

 

Keywords: Piper betle, S. aureus, Thioredoxin reductase B, trxB, Catalase, Kata, RT-PCR

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Author Biography

Roslinah Mohamad Hussain, Universiti Teknologi MARA
Senior Lecturer Department of Medical Laboratory Technology Reserach Interests: Molecular Microbiology; Natural Products

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How to Cite
Hussain, R. M., F. K. Adam Ho, M. Mustakim, and H. Suhaimi. “UPREGULATION OF TrxB EXPRESSION BY PIPER BETEL LINN ETHANOLIC EXTRACT SUGGESTS INDUCTION OF OXIDATIVE STRESS WHICH POTENTIALLY CONTRIBUTES TO KILLING OF STAPHYLOCOCCUS AUREUS IN VITROUPREGULATION OF TRXB EXPRESSION BY PIPER BETEL LINN ETHANOLIC EXTRACT SU”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 2, Dec. 2014, pp. 154-9, https://innovareacademics.in/journals/index.php/ijpps/article/view/3485.
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