CYTOTOXICITY STUDIES OF AN ETHNOBOANICALLY SELECTED SOLANUM PUBESCENS WILLD. AGAINST SELECTED HUMAN CANCER CELL LINES

  • Mani Ramakrishnan Dr. Mani Ramakrishnan Adjunct Faculty - EVS Presidency University Bangalore, Karnataka, India
  • Sudhama V N
  • Sudhama V N

Abstract

Objective: The present study was aimed to determine the cytotoxicity concentration (CTC50) of different extracts made from the leaf and stem bark of an ethno botanically selected S. pubescens against Hep G2 (Human liver carcinoma), CaCo2 (Human colon carcinoma) and T-47 D (Human breast cancer) cell lines.


Methods: Ethnobotanical survey was done through interviewing traditional medicinal practitioners then a potential herbal plant was selected after a thorough literature survey and its identity was confirmed. The soxhlet extraction method was adopted using five different solvents from leaf and stem bark powders of the study plant and the CTC50 of all the extracts were determined by MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidez] assay method.


Results: Among the different extracts, CTC50 values were significant for stem bark extracts made from methanol (24.51±0.08 µg/ml) against Hep G2, while leaf chloroform extract was promising (57.15 ±1.75 µg/ml) against CaCo2 and n-hexane extract of leaf exhibited significant value (20.27±1.52 µg/ml) against T-47 D cancer cell lines.


Conclusion: Major findings of the present study clearly provide evidence that the leaf and stem bark of S. pubescens possesses the potential anticancer bioactive compound solasodine.

Keywords: S. pubescens, cytotoxic activity, MTT assay, Hep G2, CaCo2, T-47 D

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Author Biography

Mani Ramakrishnan, Dr. Mani Ramakrishnan Adjunct Faculty - EVS Presidency University Bangalore, Karnataka, India
School of Engineering

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Ramakrishnan, M., S. V N, and S. V N. “CYTOTOXICITY STUDIES OF AN ETHNOBOANICALLY SELECTED SOLANUM PUBESCENS WILLD. AGAINST SELECTED HUMAN CANCER CELL LINES ”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 1, Nov. 2019, https://innovareacademics.in/journals/index.php/ijpps/article/view/35594.
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