PRELIMINARY PHYTOCHEMICAL SCREENING, TOTAL PHENOL AND FLAVONOID CONTENT OF MIMOSA RUBICAULIS AND REINWARDITA INDICA
Objective: Phytochemicals as phenol and flavonoid have a powerful biological activity. So, this study aimed to carry out phytochemical screening, total phenol and flavonoid content in two plant species i.e. M. rubicaulis and R. indica.
Methods: The extraction of different parts of two plant species was done by maceration using ethanol. Phytochemical screening was done to confirm the presence of phytochemicals. Total phenol content was done by Folin ciocalteu method and total flavonoid content was done by Aluminium chloride colorimetric method.
Results: Phytochemical analysis revealed the presence of flavonoid, phenol, terpenoids in both plant species. The highest concentration of phenol content was observed in the root and stem of an extract of M. rubicaulis i.e. 281.83±1.98 mg GAE/g dry extract weight and 225.37±0.60 mg GAE/g dry extract weight. The highest concentration of flavonoid contents was observed in the leaves of R. indica i.e. 462.21±4.67 mg QE/g dry extract weight followed by stem and root of M. rubicaulis i.e. 381.06±5.23 mg QE/g dry extract weight and 337.43±1.39 mg QE/g dry extract weight.
Conclusion: Phytochemical analysis concluded the presence of biologically important phytoconstituents like flavonoid and phenol in both plant species. Further studies, should be carried out to isolate specific chemical constituents and should be used in different studies to explore their biological effects.
2. Newman DJ, Cragg GM, Snader KM. Natural products as a source of new drugs over the period. J Nat Prod 2003;66:1022-37.
3. Yadav RNS, Agarwala M. Phytochemical analysis of some medicinal plants. J Phytol 2011;3:10-4.
4. Rawat P, Bachheti RK, Kumar N, Rai N. Phytochemical analysis and cvaluation of in vitro immunomodulatory activity of Rhododendron arboreum leaves. Asian J Pharm Clin Res 2018;11:123-8.
5. Figueroa LA, Navarro LB, Vera MP, Petricevich VL. Antioxidant activity, total phenolic and flavonoid contents, and cytotoxicity evaluation of Bougainvillea xbuttiana. Int J Pharm Pharm Sci 2014;6:497-2.
6. Antolovich M, Prenzler PD, Patsalides E, Mc Donald S, Robards K. Methods for testing antioxidant activity. Analyst 2001;127:183–98.
7. Dixon RA, Dey PM, Lamb CJ. Phytoalexins: enzymology and molecular biology. Adv Enzymol Relat Areas Mol Biol 1983;55:69.
8. Nijveldt RJ, Van Nood EL, Van Hoorn DE, Boelens PG, Van Norren K, Van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr 2001;74:418-25.
9. Khan AM, Qureshi RA, Faizan Ullah F, Shinwari ZK, Khan J. Flavonoids distribution in selected medicinal plants of margalla hills and surroundings. Pak J Bot 2012;44:1241-5.
10. Khan AM, Qureshi RA, Ullah F, Gilani SA, Nosheen A, Sahreen S, et al. Phytochemical analysis of selected medicinal plants of margalla hills and surroundings. J Med Plants Res 2011;5:6017-23.
11. Luis A, Domingues F, Gil C, Duarte AP. Antioxidant activity of extracts of portuguese shrubs: Pterospartum tridentatum, Cytisus scoparius and Erica spp. J Med Plants Res 2009;3:886-93.
12. Misra MC. Drug treatment of hemorrhoids. Drugs 2005;65:1481-91.
13. Shakeri A, Hazeri N, Vlizadeh J, Ghasemi A, Tavallaei FZ. Phytochemical screening, antimicrobial and antioxidant activities of Anabasis aphylla L. extracts. Kragujevac J Sci 2012;34:71-8.
14. Manandhar NP. Plants and people of Nepal. Portland: Timber Press; 2002. p. 117, 320, 421.
15. Kodangala C, Saha S, Kodangala P. Phytochemical studies of aerial parts of the plant Leucas lavandulaefolia. Pharm Chem 2010;2:434-7.
16. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5:1142-5.
17. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
18. Dutta J. Phytochemical analysis and TLC fingerprinting of methanolic extract of three medicinal plants. Int Res J Pharm 2013;4:123-6.
19. Kala C, Ali SS, Chaudhary S. Comparative pharmacognostical evaluation of Costus speciosus (Wild ginger) and Zingiber officinale (ginger) rhizome. Int J Curr Pharm Res 2016;8:19-23.
20. Alawiyah AL, Achmadi SS, Syahbirin G. Total phenolic content and flavone bioactivity of peanut hulls as antioxidant and antiproliferation toward Henrietta Lacks cancer cells. Asian J Pharm Clin Res 2017;10:144-9.
21. Rawat A, Rana PS, Molpa D, Saklani P. Phytochemical composition and antioxidant activity of Reinwardtia indica from a selected location of Uttrakhand. Int J Prog Eng Manag Sci Hum 2015;1:22-9.
22. Jebitta SR, Allwin SJ. Antioxidant activity, total phenol, flavonoid and anthocyanin contents of jamun (Syzygium cumini) pulp powder. Asian J Pharm Clin Res 2016;9:361-3.
23. Atanassova M, Georgieva S. Comparative polyphenol composition and antioxidant capacity of the Bulgarian plants (Dry Herbs). Electron J Environ Agric Food Chem 2010;9:1514-23.
24. Aryal S, Baniya MK, Danekhu K, Puspa Kunwar, Gurung R, Koirala N. Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants 2019;8:96.
25. Bahorun T, Luximon Ramma A, Crozier A, I Aruoma O. Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of mauritian vegetables. J Sci Food Agric 2004;84:1553-61.
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