ANTIOXIDANT AND HEPATOPROTECTIVE EFFECT OF QUERCUS ILEX LEAVES EXTRACT IN ETHANOL INDUCED LIVER DAMAGE IN WISTAR RATS

  • RENU MALIK Lord Shiva College of Pharmacy, Sirsa, Haryana, India
  • K. G. SINGHAL ASBASJSM College of Pharmacy, Bela, Ropar, Punjab, India

Abstract

Objective: This study was undertaken to investigate the hepatoprotective and antioxidant activity of Quercus ilex leaves extract (QILE) on ethanol-induced toxicity in Wistar rats.


Methods: Hepatotoxicity was induced by administering ethanol (40%) at a dose of 7.9 gm/kg/day; p. o. (1:1 of ethanol in olive oil) for 28 d. Silymarin 100 mg/kg/day; p. o. was used as a standard drug. The whole study was divided into a prophylactic and curative study. In the prophylactic study, the Silymarin and QILE (test drug) 100, 200, and 400 mg/kg Body Weight(BW) given orally one hour before administration of 40% ethanol administration for 28 d. In the curative study, 7 d of treatment of Silymarin and QILE 200 and 400 mg/kg BW was given orally after 28 d of ethanol administration to different groups.


Results: Hepatoprotectivity was confirmed by the highly significantly (p<0.001) restoration of elevated biochemical parameters like SGPT, SGOT, ALP, TB, and highly significantly (p<0.001) depleted Albumin and Total protein levels by 200 mg/kg BW QILE in comparison to the positive control group. QILE 200 mg/kg highly significantly (p<0.001) raised the antioxidants by draining the elevated oxidative stress markers in comparison of positive control group. At dose levels QILE 200 mg/kg, significant (p<0.05) protection from loss in body weight and in liver weight was found when the comparison was done with the positive control group. Histopathology revealed that QILE 200 mg/kg reduced the markers of cell necrosis.


Conclusion: Present study revealed that Quercus ilex leaves have antioxidant and hepatoprotective activity due to its chemical constituents.

Keywords: Hepatotoxicity, Ethanol, Silymarin, Quercus ilex, Antioxidant

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MALIK, R., and K. G. SINGHAL. “ANTIOXIDANT AND HEPATOPROTECTIVE EFFECT OF QUERCUS ILEX LEAVES EXTRACT IN ETHANOL INDUCED LIVER DAMAGE IN WISTAR RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 4, Mar. 2020, pp. 26-31, doi:10.22159/ijpps.2020v12i4.36689.
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