Modulation of Cell-Cycle gene expression by Chitosan coated Silver nanopartices on Human Epidermoid Carcinoma cells.MODULATION OF CELL-CYCLE GENE EXPRESSION BY CHITOSAN COATED SILVER NANOPARTICES ON HUMAN EPIDERMOID CARCINOMA CELLS

  • Muppalaneni Pavani SASTRA University
  • Sundaramurthy Dhakshinamoorthy SASTRA University
  • Kuppuswamy Ashok Ayyappa Assistant Professor( Research) Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University


Objective: To detect the variability in the expression of cell cycle genes such as Cyclin D1,CDK4 p21,p27 and p57 associated with cytotoxicity of Human epidermoid carcinoma cells using qPCR.

Methods: The synthesis silver nanoparticles and chitosan coated silver nanoparticles was done by chemical reduction method using polyvinyalpyrolidone (PVP) as surfactant and glucose as reducing agent. The cell proliferation assay was performed using MTS assay. Gene expression analysis was performed using Eppendorf Realplex 2 PCR systems.

Results: The lowest concentration of 1mg of Chitosan-AgNPs with 25μg/ml concentration showed 35% reduction in the A431 cell line growth. Significantly increased expression of p21, p27, p57, CCND1 and CD4 gene was observed among 1mg of Chitosan-AgNPs with 25μg/ml concentrations indicating potential apoptotic activity at the lowest concentration of Chitosan-AgNPs on A431 cells.

Conclusions: The effect of AgNPs inducing apoptosis/growth arrest at low concentrations (1mg-25μg/ml) has been more effective while coating the nanoparticle with chitosan. The potential apoptotic properties of Chitosan-AgNPs are evident by the significant up regulation of the cell cycle genes when treated with low concentration of Chitosan-AgNPs.


Keywords: Silver nanoparticles, Cell Cycle genes, Apoptosis, Chitosan, Gene expression


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Author Biography

Kuppuswamy Ashok Ayyappa, Assistant Professor( Research) Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University

Gained 5 years of research experience in Genetics of type 2 diabetes and dyslipidemia.  My research training as part of the PhD studies consisted of both clinical /population based biochemical studies and Molecular genetic studies involving screening of Novel genetic variants associated with low HDL and High Triglyceride levels among diabetic and non-diabetic subjects.  I have screened over 25 SNPs spanning 5 candidate genes implicated in dyslipidemia and type 2 diabetes.  Determination of haplotypes that could be used as predictive tool in the inheritance of risk was also assessed in my research pertaining to low HDL risk and High Triglyceride risk.

My Current research work focuses on functional validation of some of the novel loci identified in the Genome Wide association studies (GWAS) linked to cardiovascular disease (CVD) using animal models, and regulation of these loci by specific miRNA’s.



Assistant Professor (Research),

Department of Biotechnology


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How to Cite
Pavani, M., S. Dhakshinamoorthy, and K. A. Ayyappa. “Modulation of Cell-Cycle Gene Expression by Chitosan Coated Silver Nanopartices on Human Epidermoid Carcinoma cells.MODULATION OF CELL-CYCLE GENE EXPRESSION BY CHITOSAN COATED SILVER NANOPARTICES ON HUMAN EPIDERMOID CARCINOMA CELLS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 2, Dec. 2014, pp. 355-9,
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