POTENCY OF Γ-ORYZANOL-RICH BLACK RICE BRAN (ORYZA SATIVA L. INDICA) EXTRACT FOR TYROSINASE INHIBITION

  • AFIFAH VARDHANI Magister Program of Herbal Medicine, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, 16424 Indonesia
  • MAHDI JUFRI Laboratory of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, 16424 Indonesia
  • ERNI PURWANINGSIH Department of Pharmacy, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia

Abstract

Objective: The objectives of this study were to quantify γ-oryzanol in an ethanolic extract of Oryza sativa L. Indica (black rice) bran and to evaluate its activity as a tyrosinase inhibitor.


Methods: Black rice bran was extracted via maceration in 96% ethanol, and the γ-oryzanol concentration in the extract was measured through high-performance liquid chromatography. The applicability of the extract as a skin lightening agent was determined by evaluating its tyrosinase inhibition activity.


Results: The dry rice bran contained 118.572 mg/g of γ-oryzanol, and the extract inhibited tyrosinase activity at an IC50 of 74.8%.


Conclusion: The black rice bran extract was sufficiently potent for use in skin lightening formulations.

Keywords: Black rice bran, Gamma oryzanol, Tyrosinase inhibitor, Lightening agent

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Author Biographies

MAHDI JUFRI, Laboratory of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, 16424 Indonesia

Faculty of Pharmacy

ERNI PURWANINGSIH, Department of Pharmacy, Faculty of Medicine, Universitas Indonesia, Salemba, Jakarta, Indonesia

Faculty of Medicine

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VARDHANI, A., M. JUFRI, and E. PURWANINGSIH. “POTENCY OF Γ-ORYZANOL-RICH BLACK RICE BRAN (ORYZA SATIVA L. INDICA) EXTRACT FOR TYROSINASE INHIBITION”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 5, Mar. 2020, pp. 90-93, doi:10.22159/ijpps.2020v12i5.37197.
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