HPLC ANALYSIS AND ANTI-INFLAMMATORY PROPERTIES STUDIES OF TRUNK BARKS OF ACACIA NILOTICA VAR ADANSONII (GUILL AND PERR) O KTZE (MIMOSACEAE)

  • BOLY A. GILCHRIST L. Laboratoire du Development Des Medicaments (LADME), Ecole Doctorale de la Sante, Universite Joseph KI-ZERBO, BP 7021, Burkina Faso
  • OUEDRAOGO NOUFOU Departement Medecine et Pharmacopee Traditionnelles Pharmacie (MEPHATRA-PH)/Institut de Recherche en Sciences de la Sante (IRSS/CNRST), 03 BP 7047 Ouaga 03, Burkina Faso
  • TRAORE ARISTIDE Departement Medecine et Pharmacopee Traditionnelles Pharmacie (MEPHATRA-PH)/Institut de Recherche en Sciences de la Sante (IRSS/CNRST), 03 BP 7047 Ouaga 03, Burkina Faso
  • TRAORE KADIATOU T. Laboratoire du Development Des Medicaments (LADME), Ecole Doctorale de la Sante, Universite Joseph KI-ZERBO, BP 7021, Burkina Faso
  • BELEMLILGA MOHAMED Departement Medecine et Pharmacopee Traditionnelles Pharmacie (MEPHATRA-PH)/Institut de Recherche en Sciences de la Sante (IRSS/CNRST), 03 BP 7047 Ouaga 03, Burkina Faso, Laboratoire du Development Des Medicaments (LADME), Ecole Doctorale de la Sante, Universite Joseph KI-ZERBO, BP 7021, Burkina Faso
  • BELEMNABA LAZARE Laboratoire du Development Des Medicaments (LADME), Ecole Doctorale de la Sante, Universite Joseph KI-ZERBO, BP 7021, Burkina Faso
  • OUEDRAOGO MOUSSA Laboratoire du Development Des Medicaments (LADME), Ecole Doctorale de la Sante, Universite Joseph KI-ZERBO, BP 7021, Burkina Faso
  • LOMPO MARIUS Departement Medecine et Pharmacopee Traditionnelles Pharmacie (MEPHATRA-PH)/Institut de Recherche en Sciences de la Sante (IRSS/CNRST), 03 BP 7047 Ouaga 03, Burkina Faso
  • OUEDRAOGO SYLVIN Departement Medecine et Pharmacopee Traditionnelles Pharmacie (MEPHATRA-PH)/Institut de Recherche en Sciences de la Sante (IRSS/CNRST), 03 BP 7047 Ouaga 03, Burkina Faso
  • GUISSOU I. PIERRE Departement Medecine et Pharmacopee Traditionnelles Pharmacie (MEPHATRA-PH)/Institut de Recherche en Sciences de la Sante (IRSS/CNRST), 03 BP 7047 Ouaga 03, Burkina Faso, Laboratoire du Development Des Medicaments (LADME), Ecole Doctorale de la Sante, Universite Joseph KI-ZERBO, BP 7021, Burkina Faso

Abstract

Objective: The objective of this study was to evaluate the in vitro and in vivo anti-inflammatory properties of the aqueous extract and fractions of the trunk bark of Acacia nilotica.


Methods: A maceration of the powder of the trunks barks of the plant was realized. Then the aqueous macerate obtained was fractionated with dichloromethane, butanol and ethyl acetate successively. The phenolic compounds of the aqueous extract, butanol and ethyl acetate fractions were identified by HPLC/DAD. Lipoxygenase and phospholipase inhibition tests with the aqueous extract and the butanol and ethyl acetate fractions were carried out. The anti-inflammatory potential of the aqueous extract was assessed in vivo by the anti-edema test with carrageenan and the analgesic test with acetic acid at different doses (200 mg/ml; 400 mg/ml; 600 mg/ml). Aspirin (200 mg/ml) and paracetamol (200 mg/ml) were used as a reference.


Results: The HPLC/DAD analysis of the extracts revealed that gallic acid is the most abundant phenol acid in the extracts. The aqueous extract inhibited lipoxygenase (IC50 = 18.32±1.18 μg/ml), phospholipase (11.44±0.32% per 100 μg/ml) and cyclooxygenase (56.48±0.29% for 100 μg/ml) as well as its tested fractions. It also reduced edema and pain in the mice by more than 50% from the 400 mg/ml dose.


Conclusion: Aqueous extract of Acacia nilotica has anti-inflammatory properties. Hence its use in traditional medicine in the treatment of inflammation.

Keywords: Acacia nilotica, HPLC, Antiinflammatory, In vivo

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L., B. A. G., O. NOUFOU, T. ARISTIDE, T. K. T., B. MOHAMED, B. LAZARE, O. MOUSSA, L. MARIUS, O. SYLVIN, and G. I. PIERRE. “HPLC ANALYSIS AND ANTI-INFLAMMATORY PROPERTIES STUDIES OF TRUNK BARKS OF ACACIA NILOTICA VAR ADANSONII (GUILL AND PERR) O KTZE (MIMOSACEAE)”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 13, no. 1, Jan. 2021, pp. 40-46, doi:10.22159/ijpps.2021v13i1.37638.
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