EXTRACTION, ISOLATION AND IDENTIFICATION OF PHYTOCONSTITUENTS PRESENT IN THE HEMIONITIS ARIFOLIA LEAVES
Objective: The investigation was carried out to prepare 95 and 50% ethanol extract of Hemionitis arifolia leaves, and for the identification and quantification of the phytoconstituents present in it.
Methods: Soxhlet extraction method was followed to prepare the extracts, followed by standard phytochemical methods to identify the phytoconstituents present in it. Paper chromatography, HPLC, LC MS/MS and LCMS studies were then done for the confirmation, quantification of phytoconstituents and detection of mass values of other unidentified phytoconstituents present in the extract.
Results: Extractive values were found to be 14.6 and 15.6% w/w respectively. In the phytochemical studies, presence of carbohydrates, glycosides, saponins, flavonoids, phenolics, tannins, phytosterols and fixed oils was identified. On doing paper chromatography with Whatman No.1 filter paper using 15% acetic acid solvent system, six different spots with yellow-orange colour were detected under UV visualization. Out of the six spots, four were identified as quercetin, kaempferol, apigenin and rutin by co-chromatography. In the RPHPLC analysis, rutin, quercetin and kaempferol were identified and quantified. In the LC MS/MS analysis, rutin, apigenin and kaempferol were identified and quantified. In the LCMS analysis, ten different m/z values were found, among which m/z 609 was identified as rutin.
Conclusion: The presence of various flavonoids and related glycosides is indicative of potential for medicinal use of this plant.
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