COMBINATION OF MOMORDICA CHARANTIA AND PHYLLANTHUS AMARUS FOR HEPATOPROTECTIVE ACTIVITY IN ETHANOL AND ANTI-TUBERCULAR DRUGS INDUCED HEPATOTOXICITY IN RATS
Objective: To evaluate the synergistic protective effect of Momordica charantia and Phyllanthus amarus combination (MC+PA) of doses 200 and 400 mg/kg on the liver in different experimental models of hepatotoxicity.
Methods: The hepatoprotective activity was evaluated in ethanol and anti-tubercular drugs (isoniazid-INH, rifampicin-RIF) induced hepatotoxicity models. Hepatotoxicity in both models was induced to all groups except the normal control. Intoxicated rats were treated with silymarin and various doses of MC+PA for 8 d in ethanol-induced and 21 d in INH+RIF induced hepatotoxicity models. At the completion of study, the biochemical markers and the anti-oxidant status (SOD and MDA) were measured and also the histopathological evaluation of the liver tissue was carried out.
Results: Combination therapy remarkably reduced the elevated profile of the biochemical markers and thereby improved the anti-oxidant status, thus exhibiting the synergistic hepatoprotective effect when compared with the positive control group (p<0.001). Histopathological evaluation demonstrated that MC+PA decreased the liver damage significantly in comparison with the positive group.
Conclusion: The current work suggests that the combined extract showed synergistic effects on ethanol and anti-tubercular drugs induced hepatotoxicity models by significantly decreasing the liver damage.
2. Gulati K, Reshi MR, Rai N, Ray A. Hepatotoxicity: its mechanisms, experimental evaluation and protective strategies. Am J Pharmacol 2018;1:1-9.
3. Harshmohan. Textbook of pathology. 7thedition. Jaypee brother’s medical publishers; 2017. p. 577-623.
4. Tayal V, Kalra BS, Agarwal S, Khurana N, Gupta U. Hepatoprotective effect of tocopherol against isoniazid and rifampicin induced hepatotoxicity in albino rats. Indian J Exp Biol 2007;45:1031-6.
5. Samuel AJSJ, Kalusalingam A, Vimala AGKA, Muthumani M. Hepatoprotective activity of extracts of beta vulgaris bulb. Int J Res Ayurveda Pharm 2018;9:71-5.
6. Gupta M, Sharma S, Gautam AK, Bhadauria. Momordica charantia linn (Karela): Nature’s Silent Healer. Int J Pharm Sci Res 2011;11:32-7.
7. Meena J, Sharma RA, Rolania R. A review on phytochemical and pharmacological properties of Phyllanthus amarus schum and thonn. Int J Pharm Sci Res 2018;9:1377-86.
8. Aljohi A, Nasri SM, Ahmed N. Antiglycation and antioxidant properties of Momordica charantia. Plos One 2016;11:1-14.
9. Zahra K, Malik MA, Mughal NS, Arshad M, Sohail MI. Hepatoprotective role of extracts of Momordica charantia L. in acetaminophen-induced toxicity in rabbits. J Anim Plant Sci 2012;22:273-7.
10. OECD guidelines for the testing of chemicals, Acute oral toxicity; 2001. p. 1-26
11. Iqbal SS, Mujahid M, Kashif SM, Khahid M, Badruddeen, Arif M, et al. Protection of hepatotoxicity using Spondias pinnata by prevention of ethanol-induced oxidative stress, DNA-damage and altered biochemical markers in wistar rats. Integr Med Res 2016;5:267-75.
12. Rao CV, Rawat AKS, Singh AP, Singh A, Verma N. Hepatoprotective potential of ethanolic extract of Ziziphus oenoplia L. Mill roots against anti-tubercular drugs induced hepatotoxicity in experimental models. Asian Pac J Trop Med 2012. p. 283-8.
13. Kakker P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 1984;21:130-2.
14. Okhawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by the thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
15. Obogwu MB, Akindele AJ, Adeyemi OO. Hepatoprotective and in vivo antioxidant activities of the hydroethanolic leaf extract of Mucuna pruriens (Fabaceae) in anti-tubercular drugs and alcohol models. Chin J Nat Med 2014;12:273-83.
16. Francoz C, Glotz D, Moreau R, Durand F. The evaluation of renal function and disease in patients with cirrhosis. J Hepatol 2010;52:605-13.
This work is licensed under a Creative Commons Attribution 4.0 International License.