COMBINATION OF MOMORDICA CHARANTIA AND PHYLLANTHUS AMARUS FOR HEPATOPROTECTIVE ACTIVITY IN ETHANOL AND ANTI-TUBERCULAR DRUGS INDUCED HEPATOTOXICITY IN RATS

PURNIMA ASHOK; LAVANYA P.; HEMANTH K.; SANDESH K. M.; YUVRAJ SINGH SURANA

doi:10.22159/ijpps.2020v12i8.38020

Authors

PURNIMA ASHOK Department of Pharmacology, KLE College of Pharmacy, Bengaluru, Karnataka, India
LAVANYA P. Department of Pharmacology, KLE College of Pharmacy, Bengaluru, Karnataka, India
HEMANTH K. Department of Pharmacology, KLE College of Pharmacy, Bengaluru, Karnataka, India
SANDESH K. M. Department of Pharmacology, KLE College of Pharmacy, Bengaluru, Karnataka, India
YUVRAJ SINGH SURANA Department of Pharmacology, KLE College of Pharmacy, Bengaluru, Karnataka, India

DOI:

https://doi.org/10.22159/ijpps.2020v12i8.38020

Keywords:

Hepatoprotective, Hepatotoxicity, Ethanol, INH RIF, MC PA

Abstract

Objective: To evaluate the synergistic protective effect of Momordica charantia and Phyllanthus amarus combination (MC+PA) of doses 200 and 400 mg/kg on the liver in different experimental models of hepatotoxicity.

Methods: The hepatoprotective activity was evaluated in ethanol and anti-tubercular drugs (isoniazid-INH, rifampicin-RIF) induced hepatotoxicity models. Hepatotoxicity in both models was induced to all groups except the normal control. Intoxicated rats were treated with silymarin and various doses of MC+PA for 8 d in ethanol-induced and 21 d in INH+RIF induced hepatotoxicity models. At the completion of study, the biochemical markers and the anti-oxidant status (SOD and MDA) were measured and also the histopathological evaluation of the liver tissue was carried out.

Results: Combination therapy remarkably reduced the elevated profile of the biochemical markers and thereby improved the anti-oxidant status, thus exhibiting the synergistic hepatoprotective effect when compared with the positive control group (p<0.001). Histopathological evaluation demonstrated that MC+PA decreased the liver damage significantly in comparison with the positive group.

Conclusion: The current work suggests that the combined extract showed synergistic effects on ethanol and anti-tubercular drugs induced hepatotoxicity models by significantly decreasing the liver damage.

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