IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF ALCHORNEA CORDIFOLIA (SCHUMACH AND THONN.) MULL. ARG. AND ANTROCARYON KLAINEANUM PIERRE EXTRACTS
Objective: This study aims to evaluate the in vitro antioxidant, anti-inflammatory activities of the aqueous and hydroethanolic extracts recipe of Alchornea cordifolia and Antrocaryon klaineanum. A preliminary phytochemical screening was carried out.
Methods: The total phenols content was determined by the Folin Ciocalteu reagent method, while the antioxidant activity of both extracts was characterized by the 2-2diphenyl-1-picrilhidrazil (DPPH) and β-carotene assays. The anti-inflammatory activity of the extracts was evaluated as the inhibition of Bovine Serum Albumin (BSA) denaturation and antiproteinase activity.
Results: The aqueous extracts of Alchornea cordifolia and Antrocaryon klaineanum contained more polyphenols [270 mg Ascorbic acid equivalent (AAE)/g dry weight (dw)] than the hydroethanolic recipe extract (262.41 mg AAE/g dw) at the same concentration level. On the other hand, the aqueous and hydroethanolic recipe extract had the same radical scavenging activity with the antiradical power of 0.851 and 0.830, respectively. Similarly, the recipe extract had the same reducing activity with reducing the power of 94.2±2.03 mg EAA/g dw and 97.4±4.16 mg EAA/g dw for the aqueous and hydroethanolic recipe extract of Alchornea cordifolia and Antrocaryon klaineanum respectively. For the anti-inflammatory activity it was observed that both extracts possess the same activity as Diclofenac® with an IC50 of 50.21 μg/ml. The phytochemical screening of the extracts revealed the presence of alkaloids, flavonoids, carbohydrates, phenols and tannins, which may account for their activities.
Conclusion: The plant recipe extract studied possess antioxidant and anti-inflammatory potentials, which may be beneficiary to its consumers.
2. Nourshargh S, Hordijk PL, Sixt M. Breaching multiple barriers: leukocyte motility through venular walls and the interstitium. Nat Rev Mol Cell Biol 2010;11:366-78.
3. McDonald B, Kubes P. Leukocyte trafficking. Inflammatory diseases of blood vessels. Department of physiology and pharmacology, faculty of medicine, university of calgary, Canada; 2012. p. 28.
4. Sosa S, Balick MJ, Arvigo R, Esposito RG, Pizza C, Altinier G, Tubaro A. Screening of the topical anti-inflammatory activity of some central american plants. J Ethnopharmacol 2002;81:211-5.
5. Reddy JS, Rao GD, Lakshmi GR. A review on the anti-arthritic of some medicinal plants. J Glo Tred Pharma Sci 2014;5:2061-73.
6. Dibong SD, Mvogo Ottou PB, Vandi D, Ndjib RC, Monkam Tchamaha F, Mpondo Mpondo E. Ethnobotany of anti hemorrhoidal medicinal plants in the markets and villages of the center and the coast. Cam J Appl Biosci 2015;96:9072-93.
7. Noumi E, Yomi A. Medicinal plants used for intestinal diseases in mbalmayo region, central province. Cameroon Fit 2001;72:246–54.
8. Banzouzi JT, Prado R, Menan H, Valentin A, Roumestan C, Mallie M, et al. In vitro antiplasmodial activity of extracts of Alchornea cordifolia and identification of an active constituent: ellagic acid. J Ethnopharmacol 2002;81:399–401.
9. Osadebe PO, Okoye FBC. Anti-inflammatory effects of crude methanolic extract and fractions of Alchornea cordifolia leaves. J Ethnopharmacol 2003;89:19–4.
10. Nia R, Paper DH, Franz G, Essien EE. Anti-angiogenic, anti-inflammatory and anti-oxidant potential of an African recipe: Alchornea cordifolia seeds. Acta Hortic 2005;678:91–6.
11. Mavar Manga H, Brkic D, Marie DE, Quetin Leclercq J. In vivo anti-inflammatory activity of Alchornea cordifolia (Schumach and Thonn.) Mull. Arg. (Euphorbiaceae). J Ethnopharmacol 2004;92:209–14.
12. Ajibesin KK, Ekpo BA, Bala DN, Essien EE, Adesanya SA. Ethnobotanical survey of akwa Ibom State of Nigeria. J Ethnopharmacol 2015;3:115-20.
13. Ajibade TO, Olayemi FO. Reproductive and toxic effects of methanol extract of Alchornea cordifolia leaf in male rats. Andrologia 2015;47:1034–40.
14. Chinsembu KC. Plants as antimalarial agents in sub-saharan Africa. Acta Trop 2015;152:32–8.
15. Siwe Noundou X, Krause RW, Van Vuuren SF, Ntinteh DT, Olivier DK. Antibacterial seffects of Alchornea cordfolia (Schumach. and Thonn.) Müll. Arg. extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens. J Ethnopharmacol 2016;179:76–2.
16. Siwe Noundou X, Thommas M, Musyoka, Vuyani M, Derek T, Ndinteh, et al. Anti-HIV-1 integrase potency of methylgallate from Alchornea cordifolia using in vitro and in silico approaches. Sci Rep 2019;9:47-8.
17. Agbor GA, Talla L, Ngongang JY. The antidiarrhoeal activity of Alchornea cordifolia leaf extract. Phytother Res 2004;18:873-6.
18. Tona L, Kambu K, Ngimbi N, Mesia K, Penge O, Lusakibanza M, et al. Antiamoebic and spasmolytic activities of extracts from some antidiarrhoeal traditional preparations used in Kinshasa Congo. Phytomedicine 2000;7:31–8.
19. Wang BC, Sork VL, Leong M, Smith BT. Hunting of mammals reduces 165 seed removal and dispersal of the afrotropical tree Antrocaryon klaineanum (Anacardiaceae). Biotropica 2007;39:340-7.
20. Douanla PD, Kuiate Tabopda T, Tchinda AT, Ewa Cieckiewicz C, Fekam Boyom MFF, Tsabang N, et al. Antrocarines A–F, antiplasmodial ergostane steroids from the stem bark of Antrocaryon klaineanum. Phytochem Rev 2015;117:521–6.
21. Kemeuze VA, Nkongmeneck BA. Antrocaryon klaineanum pierre. In: Lemmens RHMJ, Louppe D, Oteng Amoako AA. (Eds.) Prota 7: Timbers/Bois d’œuvre 2. [CD-Rom]. PROTA, Wageningen: Netherlands; 2011. p. 85–8.
22. Tchinda AT, Ngono ARN, Tamze V, Jonville MC, Cao M, Angenot L, et al. Antiplasmodial alkaloids from the stem bark of Strychnos malacoclados. Planta Med 2012;78:377–82.
23. Ndjib R, Dibong S, Mvogo P, Nyegue MA, Eto B. Ethnobotanical study of medicinal plants used in the treatment og vaginal infections in four towns in Cameroon. Haya: Saudi J Life Sci 2017;2:366-73.
24. Salau AK, Yakubu MT, Oladiji AT. Antioxidant activity of aqueous extracts of A. leiocarpus and T. avicennioides. Cam J Biol Biochem Sci 2015;23:9-16.
25. Chew YL, Goh JK, Lim YY. In vitro from the leguminosae family in penisular Malaysia. Food Chem 2009;116:13-8.
26. Brand Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate. J Food Sci Technol 1995;28:25-30.
27. Nyegue MA. Chemical and biological properties of essential oils from some aromatic and or medicinal plants in cameroon: evaluation of their anti-radical, anti-inflammatory and antimicrobial activities. Doctoral thesis of the University of Montpellier II and the University of Yaounde I; 2006. p. 194.
28. Moure A, Cruz JM, Daniel F, Dominguez M, Sineiro J, Dominguez H, et al. Natural antioxidants from residual sources. Fd Chem 2001;72:145-71.
29. Djova SV, Nyegue MA, Afagnigni AD, Etoa FX. In vitro antioxidant activity, cytotoxicity and genotoxicity of extracts from the leaves and barks of Anthocleista schweinfurthii Gilg (Loganiaceae). Asian J Res Biol Pharm Sci 2018;6:58-9.
30. Djova SV, Nyegue MA, Messi AN, Afagnigni AD, Etoa FX. Phytochemical study of aqueous extract of Ochna schweinfurthiana F. hoffm powder bark and evaluation of their anti-inflammatory, cytotoxic, and genotoxic properties. Evid Based Complement Altern Med 2019. Doi:org/10.1155/2019/8908343;8.
31. Oyedepo OO, Famurewa AJ. Antiprotease and membrane stabilizing activities of extracts of fagara zanthoxyloides, olax subscorpioides and tetrapleura tetraptera. Int J Pharmacol 1995;33:65-9.
32. Harborne JB. Phytochimical method, a guide to modern technique of plants: Chapman and Hall, London, New York; 1998. p. 10017, USA.
33. Agbor GA, Kuate D, Oben JE. Medicinal plants can be good source of antioxidants: case study in Cameroon. Pak J Biol Sci 2007;10:537-44.
34. Nga EN, Yinyang J, Baran a Bidias E, Etame Loe G, Dibong SD. Phytochemical and pharmacological study of Alchornea cordifolia (Schum. and Thonn.) Mull. Arg. and Mangifera indica in the traditional treatment of hemorrhoidal disease. J Appl Biosci 2017;109:106-19.
35. Boniface PK, Ferreira SB, Kaiser CR. Recent trends in phytochemistry, ethnobotany and pharmacological significance of Alchornea cordifolia (Schumach. and Thonn.) Muell. Arg J Ethnopharmacol 2016;191:216-44.
36. Crozier A, Clifford MN, Ashihara H. Plant secondary metabolites: occurrence, structure and role in the human diet; 2006;26:1001-13.
37. Moffarts DE, Kirschvink N, Pincemail J, Lekeux P. Impact physiologique et pathologique du stress oxydant chez le cheval. Ann Med Vet 2005;149:1-9.
38. Azzi A, Gysin R, Kempna P, Munteanu A, Villacorta L, Visarius T, et al. Regulation of gene expression by alpha-tocopherol. Biol Chem 2004;385:585-91.
39. Sen CK, Packer L. Thiol homeostasis and supplements in physical exercise. Am J Clin Nutr 2000;72:653S-69S.
40. Kirschvink N, Fievez L, Bougnet V, Art T, Degand G, Smith N, et al. Effect of nutritional antioxidant supplementation on systemic and pulmonary antioxidant status, airway inflammation and lung function in heaves affected horses. Equine Vet J 2002;34:705-12.
41. Djimeli MN, Chegaing Fodouop SP, Njateng GSS, Fokunang C, Tala DS, Kengni F, et al. Antibacterial activities and toxicological study of the aqueous extract from leaves of Alchornea cordifolia (Euphorbiaceae). BMC Complement Altern Med 2017;17:349-59.
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