POTENTIAL ROLE OF ANNONA SQUAMOSA LINN EXTRACT AND N-ACETYL CYSTEINE IN MANAGEMENT OF RIFAMPICIN-INDUCED HEPATOXICITY IN RATS

  • BOSY AZMY ABD EL MOTELP Assistant Professor of Physiology at Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Asmaa Fahmy Street Heliopolis, Cairo, Egypt
  • HADER FAWZY DARWISH Demonstrator at Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Asmaa Fahmy Street Heliopolis, Cairo, Egypt
  • SAMIHA MOHAMED ABD ELDAYEM Professor of Physiology at Zoology Department, Faculty of Women for Arts, Science and Education Ain Shams University, Asmaa Fahmy Street Heliopolis, Cairo, Egypt
  • FATMA MOKHTAR FOUDA Assistant Professor of Physiology at Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Asmaa Fahmy Street Heliopolis, Cairo, Egypt

Abstract

Objective: Rifampicin (RIF) could be a recognized therapeutic and preventive agent against tuberculosis. Still, high rates of many side effects and symptoms related to hepatotoxicity have identified during treatment. So, the current study was aimed to evaluate the antioxidant and hepatoprotective activity of methanolic extract of Annona Squamosa Linn (MEAS) and N-Acetyl Cysteine (NAC) against RIF induced hepatic injury in male rats.


Methods: The hepatoprotective effects of MEAS (500 mg/kg b.wt.) and NAC (100 mg/kg b.wt.) or co-treatment were assessed in a model of hepatotoxicity by RIF (300 mg/kg b. wt.) in male rats daily for 21 d. Moreover, bilirubin, total protein, albumin, ALT, AST, ALP, GGT, MDA, and GSH were estimated. In addition, the levels of IL-6, IL-10, 8(OH)dG, and Bcl2 were evaluated.


Results: The oral administration of MEAS and NAC or their combination resulted in significant reductions in the levels of bilirubin, albumin, hepato-specific markers namely ALT, AST, ALP, GGT, and MDA as compared to the RIF group. Furthermore, MEAS and NAC or the combination of MEAS and NAC treatment significantly up-regulated the levels of total protein, glutathione reductase with concomitant decrease in inflammatory marker level IL-6 and apoptotic marker level 8(OH)dG as well as increment the level of anti-inflammatory marker IL-10 and anti-apoptotic marker Bcl2 as compared to the RIF group. Histological examination of the liver tissue indicated that co-treatment with MEAS and NAC completely abolished the inflammation and degeneration in hepatocytes and restore the liver tissue to its normal structure.


Conclusion: The present findings demonstrated that a co-treatment of MEAS and NAC seems to be more productive and curative than alone MEAS or NAC treatment and strongly compensated the liver damage induced by RIF.

Keywords: Rifampicin, hepatotoxicity, Annona Squamosa, N-acetyl Cysteine, Inflammation, Apoptosis

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Author Biographies

BOSY AZMY ABD EL MOTELP, Assistant Professor of Physiology at Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Asmaa Fahmy Street Heliopolis, Cairo, Egypt

Department of Zoology, Faculty of Women for Arts, Science and Education, Ain Shams University.

HADER FAWZY DARWISH, Demonstrator at Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Asmaa Fahmy Street Heliopolis, Cairo, Egypt

Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University

SAMIHA MOHAMED ABD ELDAYEM, Professor of Physiology at Zoology Department, Faculty of Women for Arts, Science and Education Ain Shams University, Asmaa Fahmy Street Heliopolis, Cairo, Egypt

Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University

FATMA MOKHTAR FOUDA, Assistant Professor of Physiology at Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Asmaa Fahmy Street Heliopolis, Cairo, Egypt

Zoology Department, Faculty of Women for Arts, Science and Education, Ain Shams University, Asmaa Fahmy Street Heliopolis, Cairo, Egypt

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MOTELP, B. A. A. E., H. F. DARWISH, S. M. A. ELDAYEM, and F. M. FOUDA. “POTENTIAL ROLE OF ANNONA SQUAMOSA LINN EXTRACT AND N-ACETYL CYSTEINE IN MANAGEMENT OF RIFAMPICIN-INDUCED HEPATOXICITY IN RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 8, June 2020, pp. 117-23, doi:10.22159/ijpps.2020v12i8.38424.
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