BIOSYNTHESIS AND CHARACTERIZATION OF INTRACELLULAR TIO2NANOPARTICLES BY LACTOBACILLUS SP: AND ITS POTENTIAL APPLICATION IN DECOLOURIZATION OF METHYL ORANGE DYES
Objective: Nanotechnology is emerging as one of the most important and revolutionizing areas in the research field. Nanoparticles are produced by various methods like physical, chemical, mechanical and biological. Biological methods of microorganisms are often preferred because they are clean, non-toxic, safe, biocompatible and environmentally acceptable.
Methods: In the present studies isolation and characterization of Lactobacillus species (LAB) from Curd was done with MRS agar (Hi Media, India. M614). All the isolates were subjected to cell morphology, physiology and an array of biochemical characterization (BCT). The confirmed LAB was used for the TiO2 nanoparticles synthesis. The TiO2 nanoparticles were then characterized by Fourier Transform Infra-Red Spectroscopy (FTIR), Scanning Electron Microscope (SEM).
Results: The SEM images of samples revealed that the nanoparticles were spherical, irregularly shaped with no definite morphology. Biosynthesized TiO2 nanoparticles were ranged in size from 20 to 50 nm.
Conclusion: The presence of TiO2 nanoparticle was confirmed by face centered cubic structure of synthesized TiO2 nanoparticle was confirmed by FTIR pattern.
2. A Ahmad, P Mukherjee, S Senapati, D Mandal, MI Khan, R Kumar, et al. Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum. Colloids Surf B 2003;28:313â€“8.
3. KB Narayanan, N Sakthivel. Biological synthesis of metal nanoparticles by microbes. Adv Colloid Interface Sci 2010;156:1â€“13.
4. KB Narayanan, N Sakthivel. Phytosynthesis of gold nanoparticles using leaf extract of Coleus amboinicus Lour, Mater. Charact 2010;61:1232â€“8.
5. D Philip. Green synthesis of gold and silver nanoparticles using Hibiscus rosa sinensis, Phys. e low-dimensional syst. Nanostruct 2010;42:1417â€“24.
6. T Pradeep. Noble metal nanoparticles for water purification: a critical review. Thin Solid Films 2009;517:6441â€“78.
7. K Prasad, AK Jha. Biosynthesis of CdS nanoparticles: an improved green and rapid procedure. J Colloid Interface Sci 2010;342:68â€“72.
8. D Raghunandan, MD Bedre, S Basavaraja, B Sawle, SY Manjunath, a Venkataraman. Rapid biosynthesis of irregular shaped gold nanoparticles from macerated aqueous extracellular dried clove buds (Syzygium aromaticum) solution., Colloids Surf B 2010;79:235â€“40.
9. S Sadhasivam, P Shanmugam, K Yun. Biosynthesis of silver nanoparticles by Streptomyces hygroscopicus and antimicrobial activity against medically important pathogenic microorganisms. Colloids Surf B 2010;81:358â€“62.
10. R Sanghi, P Verma. A facile green extracellular biosynthesis of CdS nanoparticles by immobilized fungus. Chem Eng J 2009;155:886â€“91.
11. M Sathishkumar, K Sneha, Y-S Yun. Immobilization of silver nanoparticles synthesized using Curcuma longa tuber powder and extract on cotton cloth for bactericidal activity. Bioresour Technol 2010;101:7958â€“65.
12. S Sheikpranbabu, K Kalishwaralal, K-J Lee, R Vaidyanathan, SH Eom, S Gurunathan. The inhibition of advanced glycation end-products-induced retinal vascular permeability by silver nanoparticles. Biomaterials 2010;31:2260â€“71.
13. K Sneha, M Sathishkumar, S Kim, Y-S Yun. Counter ions and temperature incorporated tailoring of biogenic gold nanoparticles. Process Biochem 2010;45:1450â€“8.
14. KN Thakkar, SS Mhatre, RY Parikh. Biological synthesis of metallic nanoparticles. Nanomed 2010;6:257â€“62.
15. R Veerasamy, TZ Xin, S Gunasagaran, TFW Xiang, EFC Yang, N Jeyakumar, et al. Biosynthesis of silver nanoparticles using mangosteen leaf extract and evaluation of their antimicrobial activities. J Saudi Chem Soc 2011;15:113â€“20.
16. Z Xu, Y Peng, Y Wantai, C Jinchun. The bio-inspired approach to controllable biomimetic synthesis of silver nanoparticles in organic matrix of chitosan and silver-binding peptide (NPSSLFRYLPSD). Mater Sci Eng C 2008;28:237â€“42.
17. DS Balaji, S Basavaraja, R Deshpande, DB Mahesh, BK Prabhakar, a Venkataraman. Extracellular biosynthesis of functionalized silver nanoparticles by strains of Cladosporium cladosporioides fungus. Colloids Surf B 2009;68:88â€“92.
18. KC Bhainsa, SF Dâ€™Souza. Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus. Colloids Surf B 2006;47:160â€“4.
19. T Wang, L Yang, B Zhang, J Liu. Extracellular biosynthesis and transformation of selenium nanoparticles and application in H2O2 biosensor. Colloids Surf B 2010;80:94â€“102.
20. K Kalishwaralal, V Deepak, S Ram Kumar Pandian, M Kottaisamy, S BarathmaniKanth, B Kartikeyan, et al. Biosynthesis of silver and gold nanoparticles using Brevibacterium casei. Colloids Surf B 2010;77:257â€“62.
21. D Inbakandan, R Venkatesan, S Ajmal Khan. Biosynthesis of gold nanoparticles utilizing marine sponge Acanthella elongata (Dendy, 1905). Colloids Surf B 2010;81:634â€“9.
22. S Gurunathan, K Kalishwaralal, R Vaidyanathan, D Venkataraman, S RK Pandian, J Muniyandi, et al. Biosynthesis, purification and characterization of silver nanoparticles using escherichia coli. Colloids Surf B 2009;74:328â€“35.
23. MI Husseiny, MA El-Aziz, Y Badr, M a Mahmoud. Biosynthesis of gold nanoparticles using Pseudomonas aeruginosa. Spectrochim Acta A Mol Biomol Spectrosc 2007;67:1003â€“6.
24. JL Vivero-Escoto, II Slowing, VS-Y Lin. Tuning the cellular uptake and cytotoxicity properties of oligonucleotide intercalator-functionalized mesoporous silica nanoparticles with human cervical cancer cells HeLa. Biomaterials 2010;31:1325â€“33.
25. Z Hu, O Choi, C Yu, GE Ferna. Interactions of nanosilver with Escherichia coli cells in planktonic and biofilm cultures. Water Res 2010;1:1-9.
26. K Kalishwaralal, S Barathmanikanth, S Ram, K Pandian, V Deepak, S Gurunathan. Colloids and Surfaces Bâ€¯: Biointerfaces Silver nanoparticles impede the biofilm formation by Pseudomonas aeruginosa and Staphylococcus epidermidis. Colloids Surf B 2010;79 :340â€“4.