PARAMETRIC OPTIMIZATION OF FERULIC ACID ESTERASE PRODUCTION FROM MUCOR HIEMALIS NCIM 837PARAMETRIC OPTIMIZATION OF FERULIC ACID ESTERASE PRODUCTION FROM MUCOR HIEMALIS NCIM 837

  • Surabhi Singh Birla Institute of Technology
  • Vinod Kumar Nigam Birla Institute of Technology
  • Ashish Sachan Birla institute of technology, Mesra- Ranchi

Abstract

Objective: To optimize the parametric conditions for the production of ferulic acid esterase from Mucor hiemalis NCIM837 using HPLC for quantification.

Methods: The experiments were conducted to determine the influence of pH and temperature on ferulic acid esterase production. To optimize the suitable agricultural waste, carbon source and the nitrogen source, different agricultural wastes, nitrogen sources and carbon sources were examined. Released ferulic acid was confirmed using HPLC.

Results: Among the different agricultural waste residues screened, maize bran was identified as most suitable for ferulic acid esterase production with activity 143U/mg. Soyabean meal as the nitrogen source and sucrose as the additional carbon source was observed as the most effective in Ferulic acid ester production. Overall enzyme production increased by 2.2-fold as compared with un-optimized conditions. One unit of FAE was defined as the amount of enzyme required for release of 1µmole of FA per minute.

Conclusion: Mucor hiemalis, to the best of our knowledge, is the new addition in the list of FAE producing microorganisms. No detailed studies on FAE production using Mucor hiemalis have been published so far.

 

Keywords: Ferulic acid, Ethyl ferulate, Ferulic acid esterase, Mucor hiemalis NCIM837

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Author Biography

Ashish Sachan, Birla institute of technology, Mesra- Ranchi

Assistant professor

Dept. of Bio-engineering 

BIT Mesra, Ranchi-835215

 

References

1. Jeffries TW. Biodegradation of lignin carbohydrate complexes. Biodegradation 1990;1:163-76.
2. Barberousse H, Roiseux O, Robert C, Paquot M, Deroanne C, Blecker C. Review. analytical methodologies for quantification of ferulic acid and its oligomers. J Sci Food Agric 2008;88:1494-511.
3. Garleb KA, Fahey GC, Lewis SM, Kerley MS, Montgomery L. Chemical composition and digestibility of fiber fractions of certain by-product feedstuffs fed to ruminants. J Anim Sci 1988;66:2650-62.
4. Saulnier L, Marot C, Chanliiaud E, Thibault JF. Cell wall polysachharide interaction in maize bran. Carbohydr Polym 1995;26:279-87.
5. Tenkanen M, Schuseil J, Puls J. Production, purification and characterisation of an esterase liberating phenolic acids from lignocellulosics. J Biotechnol 1991;18:69-84.
6. Fry SC. Phenolic components of the primary cell wall. Biochem J 1982;203:493-504.
7. Kikuzaki H, Hisamoto M, Hirose K, Akiyama K, Taniguchi H. Antioxidant properties of ferulic acid and its related compounds. J Agric Food Chem 2002;50:2161-8.
8. Mathew S, Abraham TE. Ferulic acid: an antioxidant found naturally in plant cell walls and ferulic acid esterases involved in its release and their applications. Crit Rev Biotechnol 2004;24:59-83.
9. Walton NJ, Mayer MJ, Narbad A. Molecules of interest: Vanillin. Phytochem 2003;63:505-15.
10. Sigoillot C, Camarero S, Vidal T. Comparison of different fungal enzymes for bleaching high quality paper pulps. J Biotechnol 2005;115:333-43.
11. Tapin S, Sigoillot JC, Asther M. Ferulic acid esterase utilization for simultaneous processing of nonwood plants into phenolic compounds and pulp fibers. J Agric Food Chem 2006;54:3697-703.
12. MacKenzie CR, Bilous D. Ferulic acid esterase activity from Schizophyllum commune. Appl Environ Microbiol 1988;54:1170-3.
13. Nieter A, Hasse-Aschoff P, Linke D, Nimtz M, Berger RG. A halotolerant type Aferulic acid esterase from Pleurotuseryngii. Fungal Biol 2014;118348-57.
14. Zhang SB, Zhai HC, Wang L, Yu GH. Expression, purification and characterization of a ferulic acid esterase A from Aspergillus flavus. Protein Expr Purif 2013;92:36-40.
15. Wang X, Geng X, Egashira Y, Sanada H. Purification and characterization of a ferulic acid esterase from the intestinal bacterium Lactobacillus acidophilus. Appl Environ Microbiol 2004;70:2367-72.
16. Garcia-Conesa MT, Crepin VF, Goldson AJ, Williamson G, Cummings NJ, Connerton IF, et al. The ferulic acid esterase system of Talaromyces stipitatus: Production of three discrete ferulic acid esterases, including a novel enzyme, TsFaeC, with broad substrate specificity. J Biotechnol 2004;108:227-41.
17. Donaghy JA, McKay AM. Production of feruloyl/p-coumaroyl esterase activity by Penicillium expansum, Penicillium brevicompactum and Aspergillus niger. J Appl Bacteriol 1995;79:657-62.
18. Koseki T, Furuse S, Iwano K, Matsuzawa H. Purification and characterization of a ferulic acid esterase from Aspergillus awamori. Biosci Biotechnol Biochem 1998;62:2032-4.
19. Koseki T, Takahashi K, Fushinobu S, Iefugi H, Iwano K, Hashizume K, et al. Mutational analysis of a ferulic acid esterase from Aspergillus awamori involved in substrate discrimination and pH dependence. Biochim Biophys Acta 2005;1722:200-8.
20. Shin HD, Chen RR. A type b ferulic acid esterase from Aspergillus nidulans with broad pH applicability. Appl Microbiol Biotechnol 2007;73:1323-30.
21. Philippe D, Paul K, Jean-Marc J, Vincent P. Product patterns of a ferulic acid esterase from Aspergillus nidulans on large feruloyl-arabino-xylo-oligosaccharides from wheat bran. Bioresour Technol 2012;119:425-8.
22. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976;72:248-54.
23. Shin HD, Chen RR. Production and characterization of a type B ferulic acid esterase from Fusariumproliferatum NRRL 26517. Enzyme Microb Technol 2006;38:478-85.
24. Hegde S, Muralikrishna G. Isolation and partial characterization of alkaline ferulic acid esterases from Aspergillus niger CFR 1105 grown on wheat bran. World J Microbiol Biotechnol 2009;25:1963-9.
25. Topakas E, Vafiadi C, Christakopoulos P. Microbial production, characterization and applications of ferulic acid esterases. Process Biochem 2007;42:497-509.
26. Shin HD, ChenRR. Production and characterization of a type B ferulic acid esterase from Fusariumproliferatum NRRL 26517. Enzyme Microb Technol 2006;38:478-85.
27. Kumar CG, Kamle A, Mongolla P, Joseph J. Parametric optimization of ferulic acid esterase production from aspergillus terreus Strain GA2 Isolated from tropical agro-ecosystems cultivating sweet sorghum. J Microbiol Biotechnol 2011;21:947-53.
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How to Cite
Singh, S., V. K. Nigam, and A. Sachan. “PARAMETRIC OPTIMIZATION OF FERULIC ACID ESTERASE PRODUCTION FROM MUCOR HIEMALIS NCIM 837PARAMETRIC OPTIMIZATION OF FERULIC ACID ESTERASE PRODUCTION FROM MUCOR HIEMALIS NCIM 837”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 2, Dec. 2014, pp. 230-3, https://innovareacademics.in/journals/index.php/ijpps/article/view/3863.
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