XANTHINE OXIDASE INHIBITORY EFFECTS OF PISTACIA LENTISCUS L. LEAVES EXTRACTS

Authors

  • Rihab Haddi university of oran
  • Abderrazak Marouf University Center of Naama,

Keywords:

Pistacia lentiscusL, Xanthine oxydase inhibitor, TLC, Phenolic compounds, Flavonoids

Abstract

Objective: The aim of this study is to evaluate the inhibition of the xanthine oxydase (XO) by the crude extract of Pistacia lentiscus L. leaves and their subsequent fractions (chloroformic, ethyl acetate, butanolic and aqueous) in order to find a natural substitute which could have a superior effect of inhibiting XO activity and can be used as an alternative to allopurinol witch is used in therapeutic and clinical management of gout.

Methods: The degree of XO inhibitory activity was determined by measuring the absorbance spectrophotometrically at 295 nm, which is associated with uric acid formation. The different extracts were subjected to chemical screening for preliminary identification of the secondary metabolites. Phenolic acids and flavonoids detected were quantitatively determined.

Results: The results have shown that the crude and ethyl acetate extracts were found to have XO inhibitory activity reaching more than 50 % at a concentration of 100 mg/mL (ethyl acetate fraction with 60.2 %, Necessary concentration to inhibe 50% of xanthine oxidase (IC50) = 2.50, the crude with 55.3 %, IC50 = 2.57), for the butanolic fraction the inhibition reaches 17.5 %, the aqueous 15.4 % and chloroformic 4.5 %.

The comparison was also made with a positive control allopurinol at a concentration of 100μg/mL. The result shows a slight difference, allopurinol has exhibited the highest XO inhibitory activity (71.8 %).

The phytochemical tests show various fluorescence, the quantitative dosage of total phenolic compounds and flavonoids shows respectively a quantities of 246.7 mg gallic acid equivalent (GA) eq /g and 70.9 mg catechin equivalent (Cat) eq /g of lyophilized powder for the crude extract.

Conclusion: These results suggest that the crude and ethyl acetate extracts of Pistacia lentiscus leaves can be used as an alternative to allopurinol due to their high inhibition activity of XO.


 

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Author Biographies

Rihab Haddi, university of oran

departement of biology

Abderrazak Marouf, University Center of Naama,

DEPARTEMENT OF BIOLOGY

References

Ramallo IA, Zacchino SA, Furlan RLE. A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers. Phytochem Anal 2006;17(1):15-9.

Battelli MG, Corte ED, Stirpe F. Xanthine oxidase type D (dehydrogenase) in the intestine and other organs of the rat. Biochem J 1972;126(3):747-9.

Unno T, Sugimoto A, Kakuda T. Xanthine oxidase inhibitors from the leaves of Lagerstroemia speciosa (L.). J Ethnopharmacol 2004;93:391-5.

Rohman A, Riyanto S, Yuniarti N, Saputra WR, Utami R, Mulatsih W. Antioxidant activity, total phenolic, and total flavonoid of extracts and fractions of red fruit (Pandanus conoideus Lam). Int Res J 2010;17:97¬106.

Berry CE, Hare JM. Xanthine oxidoreductase and cardiovascular disease: the molecular mechanisms and pathophysiological implications. J Physiol 2004;555(3):589-606.

Pacher P, Nivorozhkin A, Szabó C. Therapeutic effects of xanthine oxidase inhibitors: renaissance half a century after the discovery of allopurinol. Pharmacol Rev 2006;58(1):87-114.

Virsaladze DK, Tetradze LO, Dzhavashvili LV, Esaliia NG, Tananashvili DE. Levels of uric acid in serum in patients with metabolic syndrome. Georgian Med News 2007;146:35-7.

Kong LD, Abliz Z, Zhou CX, Li LJ, Cheng CHK, Tan RX. Glycosides and xanthine oxidase inhibitors from Conyza bonariensis. Phytochem 2001;58(4):645-51.

Umamaheswari M, Asok Kumar K, Somasundaram A, Sivashanmugam T, Subhadradevi V, Ravi TK. Xanthine oxidase inhibitory activity of some Indian medicinal plants. J Ethnopharmacol 2007;109(3):547-51.

Fields M, Lewis CG, Lure MD. Allopurinol, an inhibitor of xanthine oxidase, reduces uric acid levels and modifies the signs associated with copper deficiency in rats fed fructose. Free Radic Biol Med 1996;20(4):595.

Kong LD, Zhang Y, Pan X, Tan RX, Cheng CHK. Inhibition of xanthine oxidase by liquiritigenin and isoliquiritigenin isolated from Sinofranchetia chinensi. Cell Mol Life Sci 2000;57:500-5.

Tapsell LC, Hemphill I, Cobiac L, Patch CS, Sullivan DR, Fenech M, et al. Health benefits of herbs and spices: the past, the present, the future. Med J Aust 2006;185(4):S4–S24.

Triggiani V, Resta F, Guastamacchia E, Sabba C, Licchelli B, Ghiyasaldin S, et al. Role of antioxidants, essential fatty acids, carnitine, vitamins, phytochemicals and trace elements in the treatment of diabetes mellitus and its chronic complications. Endocrine, Metab Immune Disorders-Drug Targets 2006;6(1):77-93.

Abd Aziz SM, Low CN, Chai LC, Abd Razak SSN, Selamat J, Son R, et al. Screening of selected Malaysian plants against several food borne pathogen bacteria. Int Food Res J 2011;18(3):1195-201.

Costantino L, Albasini A, Rastelli G, Benvenuti S. Activity of polyphenolic crude extracts as scavengers of superoxide radicals and inhibitors of xanthine oxidase. Planta Med 1992;58:342-4.

Chang WS, Lee YJ, Lu FJ, Chiang HC. Inhibitory effects of flavonoids on xanthine oxidase. Anticancer Res 1993;13:2165-70.

Selloum L, Reichl S, Muller M, Sebihi L, Arnhold J. Effects of flavonols on the generation of superoxide anion radicals by xanthine oxidase and stimulated neutrophils. Arch Biochem Biophys 2001;395(1):49-56.

Chang WS, Chiang HC. Structure-activity relationship of coumarins in xanthine oxidase inhibition. Anticancer Res 1995;15:1969-74.

Zrira S, Elamrani A, Benjilali B. Chemical composition of the essential oil of Pistacia lentiscus L. from Morocco-A seasonal variation. Flavour Fragrance J 2003;18:475-80.

Palevitch D, Yaniv Z. The effects of aqueous extracts prepared from the leaves of Pistacia lentiscus in experimental liver disease. Medicinal plants of the holy land. modan publishing house, tel aviv, israel. in ljubuncic and al. J Ethnopharmacol 2000;11:198–204.

Iauk L, Ragusa S, Rapisarda A, Franco S, Nicolosi VM. In vitro antimicrobial activity of Pistacia lentiscus L. extracts: preliminary report. J Chemother 1996;8:207–9.

Ali-Shtayeh MS, Yaghmour RMR, Faidi YR, Salem K, Al-Nuri MA. Antimicrobial activity of 20 plants used in folkloric medicine in the Palestinian area. J Ethnopharmacol 1998;60:265-71.

Sanz MJ, Milla´n MM. The dynamics of polluted air masses and ozone cycles in the western Mediterranean: relevance of forest ecosystems. Chemosphere 1998;36:1089-94.

Kawashty SA, Mosharrafa SAM, El-Gibali M, Saleh NAM. The flavonoids of four Pistacia species in Egypt. Biochem Syst Ecol 2000;28:915-7.

Romani A, Pinelli P, Galardi C, Mulinacci N, Tattini M. Identification and quantification of galloyl derivatives, flavonoid glycosides and anthocyanins in leaves of Pistacia lentiscus L. Phytochem Anal 2002;13:79–86.

Hamlat N, Hassani A, Ouafi S. Analyse des polyphénols extraits des feuilles du Pistacia lentiscus. Etude de l’activité antibactérienne. Revue Des Régions Arides 2008;21:306–16.

Villar A , Sanz MJ, Paya M. Hypotensive effect of pistacia lentiscus L. Pharm Biol 1987;25(1):1–3.

Dob T, Dahmane D, Chelghoum C. Chemical composition of the essential oils of Pistacia lentiscus L. from Algeria. J Essent Oil Res 2006;18:335–8.

Amhamdi H, Aouinti F, Wathelet JP, Elbachiri A. Chemical composition of the essential oil of pistacia lentiscus l. from eastern morocco. Rec Nat Prod 2009;3(2):90–5.

Lamnaouer D. Composition chimiques et activités biologiques de quelques plantes médicinales du PNT in Programme de l’UICN en Afrique du Nord: Phase III: Conduite d’essais d’extraction et d’analyse des huiles essentielles et des principes actifs des plantes médicinales et aromatiques. 2002.

Ali-Shtayeh MS, Yaniv Z, Mahajna J. Ethnobotanical survey in the Palestinian area: a classification of the healing potential of medicinal plants. J Ethnopharmacol 2000;73:221-32.

Chryssavgi G, Vassiliki P, Athanasios M, Kibouris Th, Michael K. Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: Evaluation of antioxydant capacity of methanolic extracts. Food Chemistry 2008;107:1120–30.

Naseem SA, Muhammad F, Muzammil HN, Kouser BM, Aurangzeb H. Activity of polyphenolic plant extracts as scavengers of free radicals and inhibitors of xanthine oxidase. J Basic Appl Sci 2006;2:1.

Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas. Second edition. Spinger –Verlag, Berlin; 1996. p. 1-384.

Heilerova L, Buckova M, Tarapcik P, Silhar S, Labuda J. Comparison of antioxidative activity data for aqueous extracts of Lemon Blam (Melissa officinalis L.), Oregano (Origanum vulgare L.), Thyme (Thymus vulgaris L.) and Agrimony (Agrimonia eupatoria L.) obtained by conventional method and the DNA-based biosensor. Czech J Food Sci 2003;21:78-84.

Kim D, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. J Agric Food Chem 2003;81:321-6.

Hamlat N, Hassani A, Ouafi S. Analyse des polyphénols extraits des feuilles du Pistacia lentiscus. Etude de l’activité antibactérienne. Revue Des Régions Arides 2008;21:306-16.

Romani A, Pinelli P, Galardi C, Mulinacci N, Tattini M. Identification and quantification of galloyl derivatives, flavonoid glycosides and anthocyanins in leaves of Pistacia lentiscus L. Phytochem Anal 2002;13:79–86.

Benhammou N, Atik Bekkara F, Kadifkova Panovska T. Antioxidant and antimicrobial activities of the Pistacia lentiscus and Pistacia atlantica extracts. Afr J Pharm Pharmacol 2008;2(2):22–8.

Villar A , Sanz MJ, Paya M. Hypotensive effect of pistacia lentiscus l. Pharm Biol 1987;25(1):1–3.

Ljubuncic P, Cogan U, Portnaya I, Azaizeh H, Said O, Bomzon A. Antioxidant activity and cytotoxicity of eight plants used in traditional Arab medicine in Israel. J Ethnopharmacol 2005;99:43-7.

Benhammou N, Atik Bekkara F, Kadifkova Panovska T. Antiradical capacity of the phenolic compounds of Pistacia lentiscus L and pistacia atlantica Desf. Adv Food Sci 2007;29(3):155–60.

Zhu JX, Wang Y, Kong LD, Yang C, Zhang X: Effects of Biota orientalis extract and its flavonoid constituents, quercetin and rutin on serum uric acid levels in oxonate-induced mice and xanthine dehydrogenase and xanthine oxidase activities in mouse liver. J Ethnopharmacol 2004;93:133-140.

Ferraz Filha ZS, Vitolo IF, Fietto LG, Lombardi JA, Saúde-Guimarães DA: Xanthine oxidase inhibitory activity of Lychnophora species from Brazil (Arnica). J Ethnopharmacol 2006;107:79-82.

Umamaheswari M, Asok-Kumar K, Somasundaram A, Sivashanmugam T, Subhadradevi V, Ravi TK: Xanthine oxidase inhibitory activity of some Indian medical plants. J Ethnopharmacol 2007;109:547-551.

Chiang HC, Lo YJ, Lu FJ: Xanthine oxidase inhibitions from leaves of Alsophila spinulosa (Hook) Tryon. J Enzyme Inhib 1994;8:61-71.

Schmeda-Hirschmann G, Zuniga J, Dutra-Behrens M, Habermehl G: Xanthine oxidase inhibitory activity of flavonoids and tannins from Hexachlamys edulis (Myrtaceae). Phytotherapy Research 1996;10:260-262.

Ponce AM, Blanco SE, Molina AS, Garcia-Domenech R, Galvez J: Study of the action of flavonoids on xanthine oxidase by molecular topology. J Chem Inf Comput Sci 2000;40:1039-45.

Lin CM, Chen CT, Chen YC. Liang and J-K: Molecular modeling of flavonoids that inhibits xanthine oxidase. Biochem Biophys Res Commun 2002;294:167-72.

Van Hoorn DEC, Nijveldt RJ, Van Leeuwen PAM, Hofman Z, M’Rabet L, De Bont DBA, et al. Accurate prediction of xanthine oxidase inhibition based on the structure of flavonoids. Eur J Pharmacol 2002;451:111-8.

Da Silva SL, Da Silva A, Honório KM, Marangoni S, Toyama MH, Da Silva ABF. The influence of electronic, steric and hydrophobic properties of flavonoid compounds in the inhibition of the xanthine oxidase. J Mol Struct (Theochem) 2004;684:1-7.

Ghosh A, Das BK, Roy A, Mandal B, Chandra G. Antibacterial activity of some medicinal plant extracts. J Nat Med 2007;62:259-62.

Sanders S, Eisenthal RS, Harrison R. NADH oxidase activity of human xanthine oxidoreductase– regeneration of superoxyde anion. Eur J Biochem 1997;245:541-8.

Cos P, Ying L, Calomme M, Hu JP, Cimanga K, Van-Poel B, et al. Structure-activity relationship and classification of flavonoids as inhibitors of xanthine oxidase and superoxide scavengers. J Nat Prod 1998;61:71-6.

Azmi SMN, Jamal P, Amid A. Xanthine oxidase inhibitory activity from potential Malaysian medicinal plant as remedies for gout. Int Food Res J 2012;19(1):159-65.

Published

01-02-2015

How to Cite

Haddi, R., and A. Marouf. “XANTHINE OXIDASE INHIBITORY EFFECTS OF PISTACIA LENTISCUS L. LEAVES EXTRACTS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 2, Feb. 2015, pp. 34-39, https://journals.innovareacademics.in/index.php/ijpps/article/view/3881.

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Review Article(s)