Evaluation of Ethosomal epigallocatechin-3-gallate gel formulations as an antioxidant and thermogenic agent

  • Walaa Sarhan Faculty of Medicine,
Zagazig University
  • Omnia Sarhan Department of pharmaceutics, faculty of pharmacy, Badr University in Cairo, Egypt


Background: Epigallocatechin-3-gallate (EGCG) is the major constituent in green tea and is determined as a natural component that could reduce the risk of obesity.

Objective: The aim of this study was to investigate in-vitro and in-vivo behavior of the prepared ethosomal formulations of green tea extract; which in turn would result in enhancing their therapeutic efficacy as antioxidants and thermogenic agents in obese mice.


Methods: Fat Wistar rats were acclimated for at least 7 days in environmentally controlled cages, following which they were divided into five groups: one received only distilled water, the second received an oral dose of green tea extract using an oral-feeding needle, for the third group green tea extract loaded gel base was applied on previously shaven dorsal side of rats and in the other 2 groups selected green tea extract loaded Ethosomal gel base (T10 and T12) were applied on previously shaven dorsal side of rats at a dose equivalent to 100 mg/kg body weight, respectively.

Results: Formulation of green tea extract as ethosomal preparations exhibited a controlled release rate due to the reservoir action of ethosomes. The levels of TBARs & MDA were lower in groups supplemented with green tea extracts compared to control group and in groups (3-5) received it transdermal (P < 0.0001 in all 3 groups and 95% CI -3.2959 to -1.5041 in-group 3) was lower than group received it orally with P = 0.0081 and 95% CI -1.3653 to -0.2347. The messenger RNA levels of UCPs 2 and 3 in BAT were increased in those 3 groups.

Conclusions: The results support efficiency of ethosomal gel in penetrating the lipid rich biological membrane. The in-vivo study confirms the antioxidant and thermogenic behavior of transdermal applied green tea extract.

Keywords: Bioavailability, lecithin, Green tea extract, transdermal, ethosome, uncoupling proteins (UCPs) and Real Time Polymerase Chain Reaction (RT-PCR)


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Sarhan, W., and O. Sarhan. “Evaluation of Ethosomal Epigallocatechin-3-Gallate Gel Formulations As an Antioxidant and Thermogenic Agent”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 11, Sept. 2020, https://innovareacademics.in/journals/index.php/ijpps/article/view/39276.
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