HEPATOPROTECTIVE EFFECT OF N-MIRACLE (POLYHERBAL FORMULATION) AGAINST ETHANOL INDUCED TOXICITY IN MALE ALBINO RATS

  • MEENATCHI SUNDARAM ANGAPPAN Department of Clinical Laboratory, National Homoeopathy Research Institute in Mental Health (Under Central Council for Research in Homoeopathy (Ccrh), Ministry of Ayush Govt of India), Sachivothamapuram Post, Kottayam 686532, Kerala, India

Abstract

Objective: The aim of this current study is to investigate the hepatoprotective efficacy of N-Miracle (a polyherbal formulation) against ethanol-induced toxicity in male albino rats.


Methods: Male Wistar albino rats weighing 150-200 g were used for the study. A total of 30 male albino rats were selected, divided into five groups. Ethanol-induced liver damage was done on Group III, IV, and V. Group I and Group II served as a normal and drug (N-Miracle) control. After the treatment period, the rats were anaesthetized by light ether anesthesia in a lethal chamber. Hepatic biomarkers, antioxidant enzymes, histopathological examination are carried out to document the hepatoprotective effect of N-Miracle (Polyherbal formulation).


Results: The results of the present study demonstrated a significant (p<0.05) increase in the levels of Aspartate Aminotransaminase (AST), Alanine Aminotransaminase (ALT) and Alkaline Phosphatase (ALP) in ethanol-induced rats as compared to normal and drug control Groups. The level of total protein and albumin were significantly (p<0.05) decreased in ethanol-treated rats. The toxic impact of ethanol was found to be restored in rats treated with N-Miracle (Polyherbal formulation). The present study also exhibited the enzymatic antioxidant efficacy of N-Miracle (Polyherbal formulation) against ethanol-induced toxicity in rats by increasing the antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase and decreasing the activity of Glutathione-S-transferase in the liver. The findings are also correlated with histopathological examination of N-Miracle treated group, which shows hepatic regeneration and decrease in degradation of hepatocytes.


Conclusion: This study could provide a possible explanation to hepatotoxicity resulting from exposure to ethanol. The findings of the present study revealed the ameliorative effect of N-Miracle (Polyherbal formulation) against ethanol-induced hepatotoxicity by improving the liver function, increasing the levels of antioxidant enzymes and restoring the morphological features of the liver.

Keywords: Ethanol, Polyherbal formulation, N-Miracle, Hepatotoxicity, Antioxidant etc

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ANGAPPAN, M. S. “HEPATOPROTECTIVE EFFECT OF N-MIRACLE (POLYHERBAL FORMULATION) AGAINST ETHANOL INDUCED TOXICITY IN MALE ALBINO RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 12, Dec. 2020, pp. 47-51, doi:10.22159/ijpps.2020v12i12.39672.
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