HEPATOPROTECTIVE EFFECT OF N-MIRACLE (POLYHERBAL FORMULATION) AGAINST ETHANOL TOXICITY IN MALE ALBINO RATS

HEPATOPROTECTIVE EFFECT OF N-MIRACLE (POLYHERBAL FORMULATION) AGAINST ETHANOL TOXICITY IN MALE ALBINO RATS

  • Meenatchi sundaram Angappan M BIOCHEMIST/RESEARCH OFFICER, DEPARTMENT OF CLINICAL LABORATORY, NATIONAL HOMOEOPATHY RESEARCH INSTITUTE IN MENTAL HEALTH (UNDER CENTRAL COUNCIL FOR RESEARCH IN HOMOEOPATHY (CCRH), MINISTRY OF AYUSH; GOVT. OF INDIA), SACHIVOTHAMAPURAM POST, KOTTAYAM-686 532, KERALA, INDIA.

Abstract

ABSTRACT


 


Objective:  The aim of this current study is to investigate hepatoprotective efficacy of N-Miracle (a polyherbal formulation) in ethanol induced toxicity in male albino rats.


 


Methods:  Male Wistar albino rats weighing 150-200 g were used for the study. A total of 30 male albino rats were selected, divided in to five groups. Ethanol induced liver damage was done on Group III, IV, and V.  Group I and Group II served as a normal and drug (N-Miracle) control. After the treatment period, the rats were anaesthetized by light ether anesthesia in lethal chamber. Hepatic bio markers, antioxidant enzymes, hispathological examination are done to document the hepatoprotective effect of N-Miracle (Polyherbal formulation).


 


Results: The results of the present study demonstrated a significant (p<0.05) increase in the levels of AST, ALT, and ALP in ethanol induced rats as compared to other Groups.  The level of total protein and albumin were significantly (p<0.05) decreased in ethanol treated rats. The toxic impact of ethanol was found to be improved in rats treated with N-Miracle (Polyherbal formulation). The present study also exhibited the enzymatic antioxidant impact of N-Miracle (Polyherbal formulation) against ethanol induced toxicity in rats by increasing the levels of enzymatic antioxidants such as superoxide dismutase, catalase, glutathione peroxidase and decreasing the activity of Glutathione-S-transferase in the liver. The findings are also supported by histopathological examination of liver which showed signs of protection against ethanol as evident from normal hepatic regeneration and decrease in degradation of hepatocytes.


 


Conclusion:  This study could provide a possible explanation to hepatotoxicity resulting from exposure to ethanol.  The findings of the present study revealed the ameliorative effect of N-Miracle (Polyherbal formulation) against ethanol induced hepatotoxicity by improving the liver function, increasing the levels of enzymatic antioxidants and restoring morphological features of liver.


 


 

Keywords: Keywords: Ethanol, polyherbal formulation, N-Miracle, hepatotoxicity, antioxidant.

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M, M. sundaram A. “HEPATOPROTECTIVE EFFECT OF N-MIRACLE (POLYHERBAL FORMULATION) AGAINST ETHANOL TOXICITY IN MALE ALBINO RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 12, no. 12, Nov. 2020, https://innovareacademics.in/journals/index.php/ijpps/article/view/39672.
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