HEPATOPROTECTIVE EFFECT OF N-MIRACLE (POLYHERBAL FORMULATION) AGAINST ETHANOL INDUCED TOXICITY IN MALE ALBINO RATS

Authors

  • MEENATCHI SUNDARAM ANGAPPAN Department of Clinical Laboratory, National Homoeopathy Research Institute in Mental Health (Under Central Council for Research in Homoeopathy (Ccrh), Ministry of Ayush Govt of India), Sachivothamapuram Post, Kottayam 686532, Kerala, India

DOI:

https://doi.org/10.22159/ijpps.2020v12i12.39672

Keywords:

Ethanol, Polyherbal formulation, N-Miracle, Hepatotoxicity, Antioxidant etc

Abstract

Objective: The aim of this current study is to investigate the hepatoprotective efficacy of N-Miracle (a polyherbal formulation) against ethanol-induced toxicity in male albino rats.

Methods: Male Wistar albino rats weighing 150-200 g were used for the study. A total of 30 male albino rats were selected, divided into five groups. Ethanol-induced liver damage was done on Group III, IV, and V. Group I and Group II served as a normal and drug (N-Miracle) control. After the treatment period, the rats were anaesthetized by light ether anesthesia in a lethal chamber. Hepatic biomarkers, antioxidant enzymes, histopathological examination are carried out to document the hepatoprotective effect of N-Miracle (Polyherbal formulation).

Results: The results of the present study demonstrated a significant (p<0.05) increase in the levels of Aspartate Aminotransaminase (AST), Alanine Aminotransaminase (ALT) and Alkaline Phosphatase (ALP) in ethanol-induced rats as compared to normal and drug control Groups. The level of total protein and albumin were significantly (p<0.05) decreased in ethanol-treated rats. The toxic impact of ethanol was found to be restored in rats treated with N-Miracle (Polyherbal formulation). The present study also exhibited the enzymatic antioxidant efficacy of N-Miracle (Polyherbal formulation) against ethanol-induced toxicity in rats by increasing the antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase and decreasing the activity of Glutathione-S-transferase in the liver. The findings are also correlated with histopathological examination of N-Miracle treated group, which shows hepatic regeneration and decrease in degradation of hepatocytes.

Conclusion: This study could provide a possible explanation to hepatotoxicity resulting from exposure to ethanol. The findings of the present study revealed the ameliorative effect of N-Miracle (Polyherbal formulation) against ethanol-induced hepatotoxicity by improving the liver function, increasing the levels of antioxidant enzymes and restoring the morphological features of the liver.

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References

Panda Amaresh, Rath Seemanchala, Pradhan Debashis, Mahanty Arpan, Gupta Bijan Kumar, Bala Nripendra Nath. Hepatoprotective activity of the whole part of the plant Cuscuta reflexa roxb. (Convolvulaceae) in chloroform, ethanol and paracetamol-induced hepatotoxic rat models. Int J Pharm Clin Res 2014;6:127-32.

Arulmozhi V, Krishnaveni M, Mirunalini S. Protective effect of Solanum nigrum fruit extract on the functional status of liver and kidney against ethanol-induced toxicity. J Biochem Tech 2012;3:339-43.

Wu D, Cederbaum AI. Alcohol oxidative stress and free radical damage. Alcohol Res Health 2003;27:277-84.

Tarasankar M, Ayaz A, Nilanjan P. Evaluation of hepatotherapeutic effects of Mikania scandens (L.) willd on alcohol-induced hepatotoxicity in rats. Int J Pharm Pharm Sci 2012;3:490-4.

Said O, Khalil K, Fulder S. Ethnobotanical survey of medicinal herbs of the middle Eastern region. J Ethnopharmacol 2002;83:251-65.

Duraipandiyan V, Ayyanar M, Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Comp Alt Med 2006;6:35-41.

Dhanaraj SK, Parasuraman S, Thing GS. Polyherbal formulation: concept of ayurveda. Pharmacogn Rev 2014;8:73–80.

Ochei J, Kolhatkar A. Medical laboratory science, theory and practice, New Delhi tata mcgraw hill publishing company limited; 2000. p. 276-7.

Reinhold JG, Reiner M. Manual determination of serum total protein, albumin, and globulin fractions by biuret method. Standard Method Clin Chem Academic Press New York; 1953. p. 88.

Reitman S, Frankel S. Glutamic–pyruvate transaminase assay by colorimetric method. Am J Clin Path 1957;28:56.

Kind PRN, King EJ. Estimation of plasma phosphatase by determination of hydrolyzed phenol with antipyrene. J Clin Pathol 1954;7:322.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Ind J Biochem Biophys 1984;21:130-32.

Sinha KA. Colorimetric assay of catalase. Anal Biochem 1972;47:389-94.

Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG. Science 1973;179:588-90.

Habig WH, Pabst MJ, Jacoby WB. Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130-9.

Gupta SP. Textbook of statistical methods. New Delhi: Sultan Chand and Sons Educational Publishers; 2014. p. 881-901.

Jadon A, Bhadauria M, Shukla S. Protective effect of Terminalia belerica Roxb. and gallic acid against CCl4 induced damage in albino rats. J Ethnopharmacol 2007;109:214-8.

Sathiavelu J, Senapathy GJ, Devaraj R. Hepatoprotective effect of chrysin on prooxidant-antioxidant status during ethanol-induced toxicity in female albino rats. J Pharm Pharmacol 2009;61:809-17.

Margaret JJ, Jayshree N. Phytochemical screening and in vitro hepatoprotective activity on the berries of Vitex agnus castus. Int J Pharm Industrial Res 2014;4:28-32.

Lizby A, Mathews D, Dhanyaraj P, Prathibhakumari V, G Prasad. Hepatoprotective and antioxidant potential of Sphaeranthus indicus [linn] on liver damage in wistar rats. Int J Pharm Pharm Sci 2012;4:222-5.

Hussain K, Scott BR, Reddy SK, Somani S. Chronic ethanol and nicotine interaction on rat tissue antioxidant defense system. Alcohol 2001;25:89-97.

Molina P, Mclain C, Villa D. Molecular pathology and clinical aspects of alcohol-induced tissue injury. Alcoholism: Clin Exp Res 2002;26:120-8.

Ji X, Zhang P, Armstrong RN, Gilliland GL. The three dimensional structure of a glutathione-s-transferase from the Mu gene class. Structural analysis of the binary complex of isoenzymes 3-3 and glutathione at 2.2–A resolution. Biochemistry 1992;31:10169-84.

Ighodaro OM, Omole JO. Ethanol-induced hepatotoxicity in male wistar rats: Effects of aqueous leaf extract of Ocimum gratissimum. J Med Med Sci 2012;3:499-505.

Published

01-12-2020

How to Cite

ANGAPPAN, M. S. “HEPATOPROTECTIVE EFFECT OF N-MIRACLE (POLYHERBAL FORMULATION) AGAINST ETHANOL INDUCED TOXICITY IN MALE ALBINO RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 12, no. 12, Dec. 2020, pp. 47-51, doi:10.22159/ijpps.2020v12i12.39672.

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