TREATMENT OF TINEA VERSICOLOR CAUSED BY MALASSEZIA FURFUR WITH DILL SEED EXTRACT: AN EXPERIMENTAL STUDY

  • Yehia A.g. Mahmoud Prof. of Mycology biotechnology
  • Metwally A. Metwally Prof. of Mycology biotechnology
  • Hanan H. Mubarak Prof. of Mycology biotechnology
  • Nesma El Zewawy Prof. of Mycology biotechnology

Abstract

Objective: Pityriasis versicolor (PV) is a superficial fungal infection of the skin characterized by changes in skin pigment due to colonization of the stratum corneum by a dimorphic lipophilic fungus of the normal flora of the skin, known as Malassezia furfur. Currently, there is no antimycotic drug against tinea versicolor with high fungicidal activity and without relapse.

Methods: Dill seed hot water extract was chosen based upon preliminary studies which included different types of essential oils and other seed extracts where it produced the highest inhibition zone of 60 mm against M. furfur. Dill seed water extract antimicrobial activity was carried out by disc diffusion method. The minimal inhibitory concentration of dill extract was estimated and found to be 6 mg/ml which were used for the formulation of ointment to treat M. furfur infected albino rabbits as an animal model.

Results: Topical application of Dill seed extract ointment (6 mg/g) two times daily succeeded in curing severe human tinea versicolor in a time course of 2 weeks, and with complete healing, which might be used in the treatment regimen after testing its safety. Histopathological effects of Dill seed extract ointment in treating rabbit skin declared no significant toxic effects. 

Conclusion: Present study indicates that dill seed extract can be used as potential candidates for preparation of antidermatophyic drug formulations and thus may be useful in the treatment of tinea versicolor inhuman.


 

Keywords: Tinea versicolor, Malassezia sp, Anethum graveolens.

Downloads

Download data is not yet available.

Author Biography

Yehia A.g. Mahmoud, Prof. of Mycology biotechnology
Botany department, Mycology research LAb.

References

1. Ayaya SO, Kamar KK. Aetiology of tinea capitis in school children. East Afr Med J 2001;10:531-5.
2. Zaitz C. Dermatoses associadas às leveduras do gênero Malassezia. An Bras Dermatol 2000;75:129-42.
3. Belec L, Testa J, Bouree P. Pityriasis versicolor in the central African Republic. J Med Vet Mycol 1991;29:323–9.
4. Abdel-Hafez A, Abdel-Aty M, Hofny RM. Prevalence of skin diseases in rural areas of Assiut Governorate, Upper Egypt. Int J Dermatol 2003;42:887-92.
5. Mubarak HM, El-Wakiel N. Pathological studies on Pityrosporum ovale and Pityrosporum orbiculare isolated from different cases of tinea versicolor. El Minia Bull 2008;19:1-25.
6. Gupta AK, Batra R, Bluhm R, Faergemann J. Pityriasis versicolor. Dermatol Clin 2003;21:413-29.
7. Mirhendi H, Makimura K, Zomorodian K, Yamada T, Sugita T, Yamaguchi H. A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species. Microbiol Methods 2005;61:281-4.
8. Gupta AK, Kohli Y, Faergemann J, Summerbell RC. Epidemiology of Malassezia yeasts associated with pityrisasis versicolor in Ontario. Canada Med Mycol 2001;39:199-06.
9. Ljubojevic S, Skerlev M, Lipozencic J, Jzbasic L. Role of Malassezia furfur in dermatology. Clin Dermatol 2002;20:179-82.
10. Makimura K, Tamura Y, Kudo M, Uchida K, Saito H, Yamaguchi H. Species identification and strain typing of Malassezia species stock strains and clinical isolates based on the DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions. J Med Microbiol 2000;49:29–35.
11. Salah SB, Makni F, Marrakchi S, Cheikhrouhou F, Bouassida S, Zahaf A, et al. Identification of Malassezia species from Tunisian patients with pityriasis versicolor and normal subjects. Mycoses 2005;48:242–5.
12. Crespo-Erchiga V, Gómez-Moyano E, CrespoKor M. Pityriasis versicolor and the yeasts of genus malassezia. J Med Mycol 2008;99:764-71.
13. Aste N, Biggio P, Pau M. Pityriasis versicolor. J Micologia Dermatol 1987;1:121 –31.
14. Al-Ismail K, Aburjai T. Antioxidant activity of water and alcohol extracts of chamomile flowers, anise seeds and dill seeds. J Sci Food Agricul 2004;84:173–8.
15. Hallal A, Benali S, Markouk M, Bekkouche K. Evaluation of the analgesic and antipyretic activities of Chenopodium ambrosioides. L Asian J Exp Biol Sci 2010;1:189-92.
16. Pridham TG, Lindenfelser LA, Shotwell OL. Antibiotics against plant disease: I. Laboratory and green house survey. Phytopathol 1956;46:568–75.
17. Nokashima T, Enoki A, Fuse G. Modification of AATCC (American Association of Textile Chemists and Colorists) test method 100 and shaken method for assay of antimicrobial activity of textiles treated with antimicrobial and deodorant reagents. Bokin Bobai 1987;15:325–37.
18. Shadomy S, Espinel-Ingroff A, Cartwright R. Laboratory studies with antifungal agents susceptibility tests and bioassays, pp. 991-999. In: Manual of Clinical Microbiology, 4th ed American Soria for Microbiology (Lenette EH, Balows A, ronmentally friendly antifouling paints. Mar. Environ. Hausler WJ, Shadomy HJ, eds). Washington, DC; 1985.
19. Faergemann JMD. Expermintal tinea versicolor in rabbits and humans with Pityrosporum orbiculare. J Invest Dermatol 1979;72:326-9.
20. Khalil MK. A new application of ofloxacin certain formulations in some surgical operations. M. Sc Thesis, Tanta University, Egypt; 2000.
21. Bancroft JD, Stevens A, Turner DR. Theory and practice of histological technique, 4th edition, Churchill, Livingstone; 1996.
22. Dahiya P, Purkayastha S. Phytochemical analysis and antibacterial efficacy of dill seed oil against multi-drug resistant clinical isolates. Asian J Pharm Clin Res 2012;5(2):62-4.
23. Pascal JD, Stanich K, Girard B, Mazza G. Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol 2002;74:101-9.
24. Venugopal P. Antidermatophytic activity of garlic in vitro. Int J Dermatol 1995;34:278-9.
25. Mahmoud YA-G. In vitro evaluation of antidermatophytic activity of Egyptian Bee propolis in combination with plant essential oils in sheep hoof plate: an experimental model. Mycobiol 2003;31(2):99-104.
26. Kalyani M, Bhuvaneshwari G, Narasimmhalu, Shameem Banu AS, Renu Mathew, Jayakumar S. Characterization and in-vitro susceptibility of malassezia species in pityriasis versicolar cases from a tertiary care centre. Res J Pharm Biol Chem Sci 2014;5(1):585-92.
27. Stavri M, Gibbons S. The antimycobacterial constituents of Dill (Anethum graveolens). Phytother Res 2005;19:938-41.
28. Jirovetz L, Buchbauer G, Stoyanova G, Georgiev EV, Damianova ST. Composition, quality control and antimicrobial activity of essential oil of long time stored dill (Anethum graveolens L) seed from Bulgaria. J Agric Food Chem 2003;51:3854–7.
29. Sohm B, Cenizo V, André V, Zahouani H, Pailler-Mattei C, Vogelgesang B. Evaluation of the efficacy of a dill extract in vitro and in vivo. Int J Cosmet Sci 2011;33(2):157-63.
Statistics
818 Views | 7065 Downloads
How to Cite
Mahmoud, Y., M. Metwally, H. Mubarak, and N. Zewawy. “TREATMENT OF TINEA VERSICOLOR CAUSED BY MALASSEZIA FURFUR WITH DILL SEED EXTRACT: AN EXPERIMENTAL STUDY”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 2, Dec. 2014, pp. 1-7, https://innovareacademics.in/journals/index.php/ijpps/article/view/3972.
Section
Editorial