ESCIN MITIGATES HYPOXIA MIMICKING NCI-H23 CELLS THROUGH MODULATION OF MMPs, HIF-1α AND HIF-2α
Objective: In this study, to investigate the effect of escin on hypoxia mimicked condition induced matrix metalloproteinases (MMPs) 2 and 9 activities and invasion in NCI-H23 cells.
Methods: Escin treated NCI-H23 cells adhesion, migration and invasion were detected by the adhesion, wound healing and boyden chamber assay respectively. The activation of proteinases was detected using zymography assay. The expression of HIF-1α and HIF-2α was evaluated by immunoblot.
Results: In the present study, it was observed that escin suppressed chemically induced hypoxia condition stimulated adhesion, migration, and invasion of NCI-H23 cells. Gelatin zymography assay showed that escin inhibited CoCl2 induced MMPs-2 and 9 activation in NCI-H23 cells. Furthermore, western blot analysis revealed that escin treatment inhibited HIF-1 and 2α expression.
Conclusion: Taken together, these results indicate that escin inhibits HIFs-α mediated MMPs-2 and 9 expression, resulting in suppression of lung cancer cell invasion that is induced by chemically induced hypoxia condition. Escin is a potential therapeutic agent for clinical use in preventing the invasion of human malignant lung tumors.
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