AMELIORATION OF STREPTOZOTOCIN-INDUCED HYPERGLYCEMIA AND DYSLIPIDEMIA THROUGH ALOE DEBRANA

  • Abdilkadir Suleyman Addis Ababa University
  • Natesan Gnanasekaran Addis Ababa University Ethiopia.
  • Seifu Daniel Addis Ababa University

Abstract

Objective: To study the antiglycemic and antidyslipidemic effects of an ethanolic extract of Aloe debrana leaf gel extract in streptozotocin-induced diabetic rats.

Methods: A. debrana leaves were collected from Enemore district; Gurage area Ethiopia. A. debrana gel was obtained from water-washed gel part of A. debrana leaves and homogenized. The resulting mucilaginous, thick and straw colored homogenate was lyophilized and extracted using 95% ethanol. Diabetes was induced in male Wistar rats by streptozotocin (53 mg/kg, i. p.). Diabetic rats were treated with glibenclamide (600µg/kg.) or A. debrana (300 mg/kg) for 28 consecutive days. The blood samples were collected at regular intervals to assess hypoglycemic effect of an ethanolic extract of A. debrana. At the end of the experiment, serum lipid profile were analyzed for all the experimental animals and compared with diabetic control.

Results: The ethanolic extract of A. debrana at 300 mg/kg has significant antiglycemic and antidislipidemic activities. The diabetic control animals exhibited a significant decrease in body weight compared with control animals. A. debrana inhibited streptozotocin-induced weight loss and significantly alter the lipid levels.

Conclusion: The ethanolic extract of A. debrana showed significant antiglycemic and antidislipidemic activities against streptozotocin-induced diabetes in rats.

 

Keywords: A. debrana, Antiglycemic, Antidyslipidemic

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Author Biography

Natesan Gnanasekaran, Addis Ababa University Ethiopia.

Associate Professor

Dept. of Medical Biochemistry

School of Medicine
College of Health Sciences

References

1. World Health Organization. Definition, diagnosis and classification of diabetes mellitus and its complications. Geneva, Switzerland; 2006.
2. Hu FB. Globalization of diabetes the role of diet, lifestyle, and genes. Diabetes Care 2011;34:1249–57.
3. International diabetes federation. The IDF Diabetes Atlas, 6th edition. Brussels; 2013.
4. Parasuraman S, Kumar E, Kumar A, Emerson S. Free radical scavenging property and diuretic effect of triglize, a polyherbal formulation in experimental models. J Pharmacol Pharmacother 2010;1:38–41.
5. Lester T, Tsega E. The pattern of adult medical admissions in addis ababa, Ethiopia. East Afr Med J 1976;53:620-34.
6. Seyoum B, Abdulkadir J, Gebregziabiher F, et al. Analysis of diabetic patients admitted to Tikur-Anbessa Hospital over eight years. Ethiop J Health Dev 1999;13(1):9-13.
7. Ducorps M, Ndong W, Jupkwo B, Belmejdoud G, Poirier JM, Watkins P, et al. Delivery of diabetes care in ethiopia: an experience from gondar. Ethiop Med J 2003;41(1):9-17.
8. Cohen P, Stern E, Rusecki Y, Zeudler A. High prevalence of diabetes in young adult Ethiopian immigrants to Israel. Diabetes Care 1988;37(6):824-8.
9. Naheed G. Knowledge, Attitudes and practices of type 2 diabetics mellitus. J Ayub Med Coll Abbottabad 2010;22(3):128-31.
10. Abebe D. Traditional medicine in Ethiopia. The attempt being made to promote it for effective and better utilization. SINET: Ethiop J Sci 1986;9:61-9.
11. Gebremariam A. Neonatal meningitis in Addis Ababa: a 10-year review. Ann Trop Paediatr 1998;18(4):279-83.
12. Debella A, Abebe D, Urga K. Traditional medicine in Ethiopia: perspectives and developmental efforts. J Ethiop Med Pract 2001;1:114–7.
13. Addis G, Abebe D, Urga K. A survey of traditional medicine in Shirika District, Arsi Zone, Ethiopia. Ethiop Pharm J 2001;19:30–47.
14. Belayneh A, Asfaw Z, Demissew S, Bussa FN. Medicinal plants potential and use by pastoral and agro-pastoral communities in erer valley of babile woreda, eastern ethiopia. J Ethnobiol Ethnomed 2012;8:42.
15. Yineger H, Yewhalaw D: Traditional medicinal plant knowledge and use by local healers in Sekoru District, Jimma Zone, Southwestern Ethiopia. J Ethnobiol Ethnomed 2007;3:24.
16. Yineger H, Kelbessa E, Bekele T, Lulekal E. Ethnoveterinary medicinal plants at bale mountains national park, Ethiopia. J Ethnopharmacol 2007;112:55-70.
17. Romila Y, Mazumder PB, Choudhury MD. A review on antidiabetic plants used by the people of manipur charactirized by hypoglycemic activity. Assam University J Sci Technol 2010;6:167-75.
18. Grace OM, Simmonds MSJ, Smith, GF, Van Wyk AE. Documented utility and biocultural value of aloe l. (Asphodelaceae): a review. Econ Bot 2009;63(2):167–78.
19. Wabuyele E. A systematic assessment of the relationship between Aloe macrocarpa and Aloe lateritia (Aloaceae). Msc thesis, Addis Ababa University, Ethiopia; 2000.
20. Sebsebe Demissew, Friis I, Tesfaye A, Wilkin P, Weber O, Bachman S, et al. Four new species of Aloe (Aloaceae) from Ethiopia, with notes on the ethics of describing new taxa from foreign countries. Kew Bull 2011;66:111–21.
21. Sebsebe Demissew, Nordal I, Stabbetorp OE. Flowers of ethiopia: aloes and other lilies shama books; Addis Ababa, Ethiopia; 2003. p. 54-5.
22. Hamman JH. Composition and Applications of Aloe vera Leaf Gel. Mol 2008;13:1599-616.
23. Ni Y, Yates KM, Tizard IR. Aloe polysaccharides. In: Aloes The Genus Aloe Reynolds T, Ed. CRC Press: Boca Raton; 2004. p. 75-87.
24. Talmadge J, Chavez J, JacobsL, Munger C, Chinnah T, Chow JT, et al. Fractionation of Aloe vera L. inner gel, purification and molecular profiling of activity. Int Immunopharmacol 2004;4:1757-73.
25. Reynolds T, Dweck AC. Aloe vera leaf gel: a review update. J Ethnopharmacol 1999;68:3-37.
26. Akbarzadeh A, Norouzia D, Mehrabi MR, Jamshidi Sh, Farhangi A, Allah A, et al. Induction of diabetes by Streptozotocin in rats. Indian J Clin Biochem 2007;22:60-4.
27. Eun-Hyung Yoo, Soo-Youn Lee. Glucose biosensors: an overview of use in clinical practice. Sensors 2010;10:4558-76.
28. Baynes JW, Thorpe SR. Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes 1999;48:1–9.
29. Graham ML, Janecek JL, Kittredge JA, Hering BJ, Schuurman HJ. The streptozotocin-induced diabetic nude mouse model: differences between animals from different sources. Comp Med 2011;61:356–60.
30. Noor A, Gunasekaran S, Soosai Manickam A, Vijayalakshmi MA. Antidiabetic activity of Aloe vera and histology of organs in streptozotocin induced diabetic rats. Curr Sci 2008;94:8-25.
31. Rehman S, Jafri SA, Hassan S, Ahmed I, Naim M. Study on antidiabetic effect of Aloe vera extract on alloxan induced diabetic rats. Libyan Agric Res Center J Int 2011;2:29-32.
32. Prasad SK, Kulshreshtha A. Qureshi TN. Antidiabetic activity of some herbal plants in streptozotocin induced diabetic albino rats. Pakistan J Nutr 2009;8:551-7.
33. Rajasekaran S, Ravi K, Sivagnanam K, Subramanian S. Beneficial effect of Aloe vera leaf gel extraction on lipid profile status in rats with sterptozocin diabetes. Clin Exp Pharmacol Physiol 2006;33:232-7.
34. Murray RK, Granner DK, Mayes PA, Victor WD Rodwell. Harper’s Illustrated Biochemistry, McGraw-Hill, 26th edition; 2003.
35. Lanjhiyana S, Kartik R, Chandra P, Kumar S, Lanjhiyana Karuppaih M. Antihyperglycemic potential of Aloe gel in experimental animal model. Ann Biol Res 2011;2:17-31.
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How to Cite
Suleyman, A., N. Gnanasekaran, and S. Daniel. “AMELIORATION OF STREPTOZOTOCIN-INDUCED HYPERGLYCEMIA AND DYSLIPIDEMIA THROUGH ALOE DEBRANA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 7, no. 2, Dec. 2014, pp. 290-3, https://innovareacademics.in/journals/index.php/ijpps/article/view/4080.
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