MICROBIAL SYNTHESIS OF SILVER NANOPARTICLES USING STREPTOMYCES SP. PG12 AND THEIR CHARACTERIZATION, ANTIMICROBIAL ACTIVITY AND CYTOTOXICITY ASSESSMENT AGAINST HUMAN LUNG (A549) AND BREAST (MCF-7) CANCER CELL LINES
Keywords:Silver nanoparticles, Streptomyces sp, Antimicrobial activity, ROS expression, human lung and breast cancer cell lines
Objective: Synthesis of silver nanoparticles using Streptomyces sp. PG12 and their characterization, antimicrobial activity and cytotoxicity against A549 and MCF-7 cancer cell lines.
Methods: The silver nanoparticles were subjected to UV-Vis. spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM/EDS), high-resolution transmission electron microscopy (HR-TEM), zeta potential, and X-ray diffractometry (XRD) analyses. Further, the antimicrobial potential was determined by using the agar well diffusion method and cytotoxicity was determined with the help of cell viability (MTT) assay and reactive oxygen species (ROS) assay.
Results: The initial indication of silver nanoparticles synthesis was noticed by the colour change in the reaction mixture and the absorption maximum at 421 nm in UV-Vis. analysis; whereas, the FTIR analysis displayed the biological functional groups responsible for the capping and stabilization of silver nanoparticles. SEM and TEM micrographs revealed the surface morphology, spherical shape, and smallest particle size as 18.91 nm. The EDS and XRD patterns confirmed the involvement of various elements during the synthesis of silver nanoparticles and the crystalline, face-centered cubic nature, respectively. The silver nanoparticles displayed considerable antimicrobial activity against human pathogens even at low MIC and MBC concentrations and exhibited increased anticancer activity against A549 and MCF-7 cell lines, where the ability of silver nanoparticles to significantly restrict the growth of tumour cells was observed at IC50 values of 69.04µg/ml and 138.30µg/ml, respectively.
Conclusion: Streptomyces sp. PG12 synthesized silver nanoparticles show significant anticancer activity against A549 and MCF-7 cell lines.
Srikar SK, Giri DD, Pal DB, Mishra PK, Upadhyay SN. Green synthesis of silver nanoparticles: a review. Green Sustainable Chem 2016;6:34-56.
Menon S, Rajeshkumar S, Venkat KS. A review on biogenic synthesis of gold nanoparticles, characterization, and its applications. Resour Effic Technol 2017;1-12.
Ahmad S, Munir S, Ullah A, Khan B, Ali J, Bilal M, et al. Green nanotechnology: a review on green synthesis of silver nanoparticles-an eco-friendly approach. Int J Nanomed 2019;14:5087-107.
Rafique M, Sadaf IM, Rafique S, Tahir MB. A review on green synthesis of silver nanoparticles and their applications. Artif Cells Nanomed Biotechnol 2017;45:1272-91.
Tarannum N, Divya, Gautam YK. Facile green synthesis and applications of silver nanoparticles: a state-of-the-art review. RSC Adv 2019;9:34926-48.
Duran N, Nakazato G, Seabra AB. Antimicrobial activity of biogenic silver nanoparticles, and silver chloride nanoparticles: an overview and comments. Appl Microbiol Biotechnol 2016;100:6555-70.
Gahlawat G, Choudhury AR. A review on the biosynthesis of metal and metal salt nanoparticles by microbes. RSC Adv 2019;9:12944-67.
de-Souza TAJ, Souza LRR, Franchi LP. Silver nanoparticles: an integrated view of green synthesis methods, transformation in the environment, and toxicity. Ecotoxicol Environ Saf 2019;171:691-700.
Garg D, Sarkar A, Chand P, Bansal P, Gola D, Sharma S, et al. Synthesis of silver nanoparticles utilizing various biological systems: mechanisms and applications-a review. Prog Biomater 2020;9:81-95.
Ali MA, Ahmed T, Wu W, Hossain A, Hafeez R, Masum MMI, et al. Advancements in plant and microbe-based synthesis of metallic nanoparticles and their antimicrobial activity against plant pathogens. Nanomater 2020;10:1146-70.
Shirley, Dayanand A, Sreedhar B, Dastager SG. Antimicrobial activity of silver nanoparticles synthesized from novel Streptomyces species. Dig J Nanomater Bios 2010;5:447-51.
Khair Allah DH, Al-Charrakh AH, Al-Dujaili NH. Antimicrobial activity of silver nanoparticles biosynthesized by Streptomyces spp. Ann Trop Public Health 2020;22:301-14.
Siegel RL, Miller KD, Jemal ADVM. Cancer statistics-2020. CA Cancer J Clin 2020;10:1-24.
Ramalingam V, Rajaram R, Premkumar C, Santhanam P, Dhinesh P, Vinothkumar P, et al. Biosynthesis of silver nanoparticles from the deep-sea bacterium Pseudomonas aeruginosa JQ989348 for antimicrobial, antibiofilm, and cytotoxic activity. J Basic Microbiol 2013;53:1-9.
Rajeshkumar S, Malarkodi C, Vanaja M, Annadurai G. Anticancer and enhanced antimicrobial activity of biosynthesized silver nanoparticles against clinical pathogens. J Mol Struct 2016;1116:165-73.
Shanmugasundaram T, Radhakrishnan M, Gopikrishnan V, Pazhanimurugan R, Balagurunathan R. A study of the bactericidal, anti-biofouling, cytotoxic and antioxidant properties of actinobacterial synthesized silver nanoparticles. Colloids Surf B 2013;111:680-7.
Wypij M, Czarnecka J, Swiecimska M, Dahm H, Rai M, Golinska P. Synthesis, characterization and evaluation of antimicrobial and cytotoxic activities of biogenic silver nanoparticles synthesized from Streptomyces xinghaiensis OF1 strain. World J Microbiol Biotechnol 2018;34:23-36.
Abd-Elnaby H, Abo-Elala G, Abdel-Raouf U, Abd-elwahab A, Hamed M. Antibacterial and anticancer activity of marine Streptomyces parvus: optimization and application. Biotechnol Equip 2016;30:180-91.
Manimaran M, Kannabiran K. Actinomycetes-mediated biogenic synthesis of metal and metal oxide nanoparticles: progress and challenges. Lett Appl Microbiol 2017;64:401-8.
Kumari S, Tehri N, Gahlaut A, Hooda V. Actinomycetes mediated synthesis, characterization, and applications of metallic nanoparticles. Inorg Nano-Metal Chem 2020.
Sreenivasa N, Meghashyama BP, Pallavi SS, Bidhayak C, Dattatraya A, Muthuraj R, et al. Biogenic synthesis of silver nanoparticles using Paenibacillus sp. in vitro and their antibacterial, anticancer activity assessment against human colon tumour cell line. J Environ Biol 2021;42:118-27.
Nayaka S, Chakraborty B, Pallavi SS, Bhat MP, Shashiraj KN, Ghasti B. Synthesis of biogenic silver nanoparticles using Zanthoxylum rhetsa (Roxb.) DC seed coat extract as reducing agent and in vitro assessment of anticancer effect on A549 lung cancer cell line. Int J Pharm Res 2020;12:302-14.
Wypij M, Jedrzejewski T, Trzcinska Wencel J, Ostrowski M, Rai M, Golinska P. Green synthesized silver nanoparticles: antibacterial and anticancer activities, biocompatibility, and analyses of surface-attached proteins. Front Microbiol 2021;12:632505.
Railean Plugaru V, Pomastowski P, Wypij M, Szultka Mlynska M, Rafinska K, Golinska P, et al. Study of silver nanoparticles synthesized by an acidophilic strain of Actinobacteria isolated from the of Picea sitchensis forest soil. J Appl Microbiol 2016;120:1250-63.
Avilala J, Golla N. Antibacterial and antiviral properties of silver nanoparticles synthesized by marine actinomycetes. Int J Pharm Sci Res 2019;10:1223-8.
Hamed AA, Kabary H, Khedr M, Emam AN. Antibiofilm, the antimicrobial and cytotoxic activity of extracellular green-synthesized silver nanoparticles by two marine-derived actinomycete. RSC Adv 2020;10:10361-7.
Silva Vinhote NM, Caballero NED, Silva TA, Quelemes PV, de-Araujo AR, de-Moraes ACM, et al. Extracellular biogenic synthesis of silver nanoparticles by actinomycetes from amazonic biome and its antimicrobial efficiency. African J Biotechnol 2017;16:2072-82.
Bizuye A, Gedamu L, Bii C, Gatebe E, Maina N. Molecular-based identification of actinomycetes species that synthesize antibacterial silver nanoparticles. Int J Microbiol 2020;1-17.
Bhat M, Chakraborty B, Kumar RS, Almansour AI, Arumugam N, Kotresha D, et al. Biogenic synthesis, characterization and antimicrobial activity of Ixora brachypoda (DC) leaf extract mediated silver nanoparticles. J King Saud Univ Sci 2021;33:101296.
Huq MA. Green synthesis of silver nanoparticles using Pseudoduganella eburnea MAHUQ-39 and their antimicrobial mechanisms investigation against drug-resistant human pathogens. Int J Mol Sci 2020;21:1510-23.
Fouda A, Hassan SE, Abdo AM, El-Gamal MS. Antimicrobial, antioxidant and larvicidal activities of spherical silver nanoparticles synthesized by endophytic Streptomyces spp. Biol Trace Elem Res 2020;195:707-24.
Sadhasivam S, Shanmugam P, Yun KS. Biosynthesis of silver nanoparticles by Streptomyces hygroscopicus and antimicrobial activity against medically important pathogenic microorganisms. Colloids Surf B 2010;81:358-62.
Dangi P, Op J. Green synthesis, characterization, and in vitro antimicrobial efficacy of silver nanoparticles synthesized from Tectona grandis wood flour. Asian J Pharm Clin Res 2019;12:257-62.
Dayma PB, Mangrola AV, Suriyaraj SP, Dudhagara P, Patel RK. Synthesis of bio-silver nanoparticles using desert isolated Streptomyces intermedius and its antimicrobial activity. J Pharm Chem Biol Sci 2019;7:94-101.
Rodriguez Louis OE, Hernandez Delgadillo R, Sanchez Najera RI, Martinez Castanon GA, Nino Martinez N, Navarro MCS, et al. Green synthesis of silver nanoparticles and their bactericidal and antimycotic activities against oral microbes. J Nanomater 2016:1-10.
Samundeeswari A, Dhas SP, Nirmala J, John SP, Mukherjee A, Chandrasekaran N. Biosynthesis of silver nanoparticles using actinobacterium Streptomyces albogriseolus and its antibacterial activity. Biotechnol Appl Biochem 2012;59:503-7.
Devagi P, Suresh TC, Sandhiya RV, Sairandhry M, Bharathi S, Velmurugan P, et al. Actinobacterial-mediated fabrication of silver nanoparticles and their broad-spectrum antibacterial activity against clinical pathogens. J Nanosci Nanotechnol 2020;20:2902-10.
Sanjivkumar M, Vaishnavi R, Neelakannan M, Kannan D, Silambarasan T, Immanuel G. Investigation on characterization and biomedical properties of silver nanoparticles synthesized by an actinobacterium Streptomyces olivaceus (MSU3). Biocatal Agric Biotechnol 2019;17:151-9.
Bharathi S, Kumaran S, Suresh G, Ramesh B, Sundari MSN. Phytosynthesis of silver nanoparticles using Hygrophila auriculata leaf extract and assessment of their antibacterial and antioxidant properties. Int J Appl Pharm 2018;10:119-25.
Al-Dhabi NA, Ghilan AKM, Esmail GA, Arasu MV, Duraipandiyan V, Ponmurugan K. Environmental friendly synthesis of silver nanomaterials from the promising Streptomyces parvus strain Al-Dhabi-91 recovered from the Saudi Arabian marine regions for antimicrobial and antioxidant properties. J Photochem Photobiol B 2019;197:111529-37.
Subbaiya R, Saravanan M, Priya AR, Shankar KR, Selvam M, Ovais M, et al. Biomimetic synthesis of silver nanoparticles from Streptomyces atrovirens and their potential anticancer activity against human breast cancer cells. IET Nanobiotechnol 2017;11:965-72.
Bhaktiari Sardari A, Mashreghi M, Eshghi H, Behnam Rasouli F, Lashani E, Shahnavaz B. Comparative evaluation of silver nanoparticles biosynthesis by two cold-tolerant Streptomyces strains and their biological activities. Biotechnol Lett 2020;42:1985-99.
Salem SS, El-Belely EF, Niedbała G, Alnoman MM, Hassan SE, Eid AM, et al. Bactericidal and in vitro cytotoxic efficacy of silver nanoparticles (Ag-NPs) fabricated by endophytic actinomycetes and their use as a coating for the textile fabrics. Nanomater 2020;10:2082-102.
Zhu B, Li Y, Lin Z, Zhao M, Xu T, Wang C, et al. Silver nanoparticles induce HePG-2 cells apoptosis through ROS-mediated signalling pathways. Nanoscale Res Lett 2016;11:198-205.
Antony A, Shrivastav N, Dubey S, Acharya A. Apoptosis inducing effect of silver nanoparticles synthesized using Magnolia champaca leaf extract on MCF-7 cell line. Int J Curr Pharm Res 2021;13:14-8.
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