STANDARDIZATION OF FRIABLE CALLUS DEVELOPMENT IN CATHARANTHUS ROSEUS (LINN.) G. DON

Authors

  • Sairam Veerabathini VELLORE INSTITUTE OF TECHNOLOGY UNIVERSITY
  • Sarang S VELLORE INSTITUTE OF TECHNOLOGY UNIVERSITY
  • Shalini S VELLORE INSTITUTE OF TECHNOLOGY UNIVERSITY
  • Deepa Sankar P VELLORE INSTITUTE OF TECHNOLOGY UNIVERSITY

Keywords:

Callus induction, Friable callus, Catharanthus roseus

Abstract

Objective: The objective of the study was to develop an effective hormonal combination for the maximum growth of callus and development of friable calli using the same medium with reduced concentration of agar.

Methods: The percentage responses of five varied growth hormonal combinations and concentrations, supplemented with Murashige and Skoog (MS)medium were recorded. The effect of casein hydrolysate on callus induction was also studied. The nature of friable calli obtained from best responsive media fortified with 0.7% and 0.6% agar was observed.

Results: The present study revealed that, three media viz., MS + 1.0 mg/L BAP + 1.0 mg/L NAA, MS + 1.5 mg/L 2,4-D + 1.0 mg/L Kin and MS + 1.5 mg/L 2,4-D + 0.5 mg/L BAP, as the best responsive media in the descending order. The effect of casein hydrolysate supplemented along with the above three media revealed MS + 1.0 mg/LBAP + 1.0 mg/L NAA + 1.0 gm/L casein hydrolysate as the best responsive media. Also, the above media supplemented with 0.6% agar was found to be the effective in terms of nature and amount of friable callus obtained.

Conclusion: The results indicated MS + 1.0 mg/L BAP + 1.0 mg/L NAA + 1.0 gm/L casein hydrolysate + 0.6% agar (85% response) as the best media for the growth and development of both callus and friable callus.

 

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Author Biography

Sairam Veerabathini, VELLORE INSTITUTE OF TECHNOLOGY UNIVERSITY

Plant Biotechnology Division

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Published

01-03-2015

How to Cite

Veerabathini, S., S. S, S. S, and D. S. P. “STANDARDIZATION OF FRIABLE CALLUS DEVELOPMENT IN CATHARANTHUS ROSEUS (LINN.) G. DON”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 7, no. 3, Mar. 2015, pp. 111-3, https://innovareacademics.in/journals/index.php/ijpps/article/view/4237.

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